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Agrisera
AGB1/Guanine nucleotide-binding protein beta 1 Polyclonal Antibody
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AGB1/Guanine nucleotide-binding protein beta 1 Antibody (AS16-3937) in WB
10 µL of protein samples from Arabidopsis thaliana were separated on SDS-PAGE and blotted 1 h to PVDF using tank transfer. Blots were blocked with Tris-buffered saline containing 0.05% Tween-20 (TBS-T) and 5% skimmed milk powder for 1 h at room temperature (RT) with agitation. The blot was incubated in the primary antibodies indicated at a dilution of 1:5,000 overnight at 4°C with agitation. The antibody so... View More
产品信息
AS16-3937
产品规格
种属反应
Plant
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from Arabidopsis thaliana AGB1 sequence.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please remember to spin the tubes briefly prior to opening them to avoid any losses that might occur from material adhering to the cap or sides of the tube.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: Arabidopsis thaliana
靶标信息
AGB1 encodes the sole canonical heterotrimeric G-protein beta subunit in Arabidopsis thaliana and is predominantly associated with the cell periphery, consistent with localization to the plasma membrane where heterotrimeric G-protein signaling is initiated and organized. The AGB1 protein is a soluble, non-enzymatic scaffold-like subunit predicted to adopt the characteristic beta-propeller fold built from seven WD40 repeats, a structural platform that mediates stable protein-protein interactions. Functionally, AGB1 forms an obligate dimer with G-protein gamma subunits (Gbetagamma) and, together with G alpha or extra-large G alpha partners, participates in signal transduction downstream of diverse surface inputs; genetic and biochemical evidence links AGB1-dependent signaling to regulation of guard cell calcium responses and stomatal movements (including coupling to phospholipase C/inositol phosphate signaling), as well as broader roles in development and stress/immune pathways such as defense against pathogens and modulation of growth under biotic or abiotic stress conditions.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ARABIDOPSIS G-PROTEIN BETA SUBUNIT; AT4G34460; ATAGB1; ELK4; ERECTA-LIKE 4; GTP binding protein beta 1; T4L20.40; T4L20_40
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