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FIGURE: 1 / 1
AGK Antibody (PA5-97316) in WB
Western blot analysis of AGK in SK-MEL-28 cells. Samples were incubated with AGK Polyclonal antibody (Product # PA5-97316) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Product Details
PA5-97316
产品规格
Species Reactivity
Human,
Rat
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 233-422 of human AGK.
Conjugate
Unconjugated
形式
Liquid
浓度
0.42 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2809118
Product Specific Information
Immunogen sequence: KAAHFFSTLK EWPQTHQASI SYTGPTERPP NEPEETPVQR PSLYRRILRR LASYWAQPQD ALSQEVSPEV WKDVQLSTIE LSITTRNNQL DPTSKEDFLN ICIEPDTISK GDFITIGSRK VRNPKLHVEG TECLQASQCT LLIPEGAGGS FSIDSEEYEA MPVEVKLLPR KLQFFCDPRK REQMLTSPTQ; Positive Samples: 293T, SH-SY5Y, HepG2, HeLa, 22Rv1, BT-474, K-562, Rat brain; Cellular Location: Mitochondrion membrane
Target Information
Lipid kinase that can phosphorylate both monoacylglycerol and diacylglycerol to form lysophosphatidic acid (LPA) and phosphatidic acid (PA), respectively. Does not phosphorylate sphingosine. Overexpression increases the formation and secretion of LPA, resulting in transactivation of EGFR and activation of the downstream MAPK signaling pathway, leading to increased cell growth.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: Acylglycerol kinase, mitochondrial; FLJ10842; hAGK; HsMuLK; multi-substrate lipid kinase; Multiple substrate lipid kinase
Gene Aliases: AGK; CATC5; CTRCT38; MTDPS10; MULK; RGD1562046
UniProt ID: (Human) Q53H12
Entrez Gene ID: (Human) 55750, (Rat) 502749
Molecular Function:
kinase
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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