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Invitrogen
AGO1 Recombinant Superclonal Antibody
高级验证
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
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AGO1 Antibody (711755) in WB
Western blot analysis was performed on whole cell extracts (30 µg lysate) of MCF-7 (Lane 1), NTERA-2 cl.D1 (Lane 2), HEL 92.1.7 (Lane 3), K-562 (Lane 4), MOLT4 (Lane 5), A-431 (Lane 6), THP-1 (Lane 7), MDA-MB-231 (Lane 8) and T-47D (Lane 9). The blots were probed with Anti-AGO1 Recombinant Rabbit Polyclonal Antibody (Product # 711755, 1-2 µg/mL). A 97 kDa band corresponding to AGO1 was observed across most of the cell lines tested. The blots were detected by chemiluminescence using Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary ... View More
产品信息
711755
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse
已发表种属
Bovine
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
抗原
Peptides corresponding to human AGO1 [1) aa17-aa35, 2) aa382-aa401]
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2692263
产品详细信息
This antibody is predicted to react with Monkey, Pig, Mouse
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
AGO1 is a member of the Argonaute family of proteins which play a role in RNA interference. The protein is highly basic, and contains a PAZ domain and a PIWI domain. It may interact with dicer1 and play a role in short-interfering-RNA-mediated gene silencing. AGO1 binds to miRNAs or siRNAs and represses the translation of mRNAs.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
生物信息学
蛋白别名: argonaute 1; Argonaute RISC catalytic component 1; Argonaute1; eIF-2C 1; eIF2C 1; Eukaryotic translation initiation factor 2C 1; eukaryotic translation initiation factor 2C, 1; Golgi Endoplasmic Reticulum protein 95 kDa; mAgo1; Piwi/Argonaute family protein meIF2C1; Protein argonaute-1; Putative RNA-binding protein Q99
基因别名: AGO1; EIF2C; EIF2C1; GERP95; hAgo1; Q99
UniProt ID: (Human) Q9UL18, (Mouse) Q8CJG1
Entrez Gene ID: (Human) 26523, (Mouse) 236511
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II core binding
core promoter binding
RNA binding
double-stranded RNA binding
single-stranded RNA binding
endoribonuclease activity
protein binding
miRNA binding
poly(A) RNA binding
nucleic acid binding
transcription regulatory region DNA binding
pre-miRNA binding
translation initiation factor
nuclear-transcribed mRNA catabolic process
transcription, DNA-templated
Wnt signaling pathway, calcium modulating pathway
RNA secondary structure unwinding
miRNA metabolic process
gene silencing by RNA
pre-miRNA processing
posttranscriptional gene silencing by RNA
production of miRNAs involved in gene silencing by miRNA
miRNA mediated inhibition of translation
mRNA cleavage involved in gene silencing by miRNA
miRNA loading onto RISC involved in gene silencing by miRNA
positive regulation of transcription from RNA polymerase II promoter
phosphatidylinositol-mediated signaling
mRNA cleavage involved in gene silencing by siRNA
regulation of transcription, DNA-templated
regulation of translation
positive regulation of gene expression
