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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • AIF Antibodies

          Invitrogen

          AIF Monoclonal Antibody (7F7AB10)

          Advanced Verification

          This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

          View all (24) AIF antibodies
          Datasheet
          Protocols
          Tech Support

          Cite AIF Monoclonal Antibody (7F7AB10)

          Datasheet
          Protocols
          Tech Support
          • Antibody Testing Data (8)
          • Advanced Verification (1)
          AIF Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 9

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          AIF Antibody (45-6200) in WB

          Western blot was performed using Anti-AIF Monoclonal Antibody (7F7AB10)(Product # 45-6200) and a 60kDa band corresponding to AIF was observed across cell lines tested. Whole cell extracts (30 µg lysate) of K-562 (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), HeLa (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Product Details

          45-6200

          Applications
          Tested Dilution
          Publications

          免疫印迹 (WB)

          2-5 µg/mL
          -

          免疫组化 (IHC)

          10 µg/mL
          -

          免疫细胞化学 (ICC/IF)

          5-10 µg/mL
          -

          流式细胞分析 (Flow)

          1 µg/mL
          -
          产品规格

          Species Reactivity

          Human

          Host/Isotype

          Mouse / IgG1, kappa

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          7F7AB10

          Immunogen

          Human heart mitochondria
          View immunogen

          Conjugate

          Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          IgG fraction

          保存液

          HEPES buffered saline

          内含物

          0.02% sodium azide

          保存条件

          4° C, do not freeze

          RRID

          AB_2533822

          Product Specific Information

          When performing IHC use heat mediated antigen retrieval before commencing with IHC staining protocol.

          When perfoming ICC or IF recommended sample treatment are paraformaldehyde fixed at 4% for 20 minutes and Triton X-100 permeabilized at 0.1% for 15 minutes.

          Target Information

          Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          References (0)

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          Let us know so we can reference it here.
          Cite this product

          Bioinformatics

          Protein Aliases: AGR1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; ERP18; ERP19; hAG-1; MGC111425; pcd8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; UNQ713/PRO1376

          View more View less

          Gene Aliases: AIF; AIFM1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8

          View more View less

          UniProt ID: (Human) O95831

          View more View less

          Entrez Gene ID: (Human) 9131

          View more View less

          Molecular Function: oxidoreductase

          Function(s)
          response to ischemia DNA catabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process response to toxic substance chromosome condensation positive regulation of apoptotic process positive regulation of neuron apoptotic process cell redox homeostasis neuron apoptotic process oxidation-reduction process intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide cellular response to estradiol stimulus cellular response to nitric oxide cellular response to oxygen-glucose deprivation response to L-glutamate regulation of apoptotic DNA fragmentation cellular response to aldosterone
          Process(es)
          DNA binding protein binding electron carrier activity NAD(P)H oxidase activity oxidoreductase activity, acting on NAD(P)H protein dimerization activity FAD binding
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          We're here to help

          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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