Invitrogen

AIF Monoclonal Antibody (7F7AB10)

高级验证

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

查看其他22个AIF抗体

说明书

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说明书

Protocols

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AIF Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of AIF was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with AIF Monoclonal Antibody (7F7AB10) (Product # 45-6200) at 5 µg in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing mitochondria localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.

AIF Antibody in Western Blot (WB) — Western blot was performed using Anti-AIF Monoclonal Antibody (7F7AB10)(Product # 45-6200) and a 60kDa band corresponding to AIF was observed across cell lines tested. Whole cell extracts (30 µg lysate) of K-562 (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), HeLa (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:500 Dilution) and detected by chemiluminescence with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A28177, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

AIF Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of AIF in HL-60 cells using an AIF Monoclonal antibody (Product # 45-6200) at 1 µg/mL, as shown in blue. Isotype control antibody is shown in red.

AIF Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with AIF siRNA and decrease in signal intensity was observed in Western Blot application using Anti-AIF Monoclonal Antibody (7F7AB10) (Product # 45-6200). {KD}

产品信息

45-6200

应用	建议稀释比	已发表文章
免疫印迹 (WB)	2-5 µg/mL	-
免疫组化 (IHC)	10 µg/mL	-
免疫细胞化学 (ICC/IF)	5-10 µg/mL	-
流式细胞分析 (Flow)	1 µg/mL	-

产品规格
种属反应	Human
宿主/亚型	Mouse / IgG1, kappa
分类	Monoclonal
类型	Antibody
克隆号	7F7AB10
抗原	Human heart mitochondria
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	IgG fraction
保存液	HEPES buffered saline
内含物	0.02% sodium azide
保存条件	4° C, do not freeze
RRID	AB_2533822

产品详细信息

When performing IHC use heat mediated antigen retrieval before commencing with IHC staining protocol.

When perfoming ICC or IF recommended sample treatment are paraformaldehyde fixed at 4% for 20 minutes and Triton X-100 permeabilized at 0.1% for 15 minutes.

靶标信息

Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: AGR1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; ERP18; ERP19; hAG-1; MGC111425; pcd8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; UNQ713/PRO1376

基因别名: AIF; AIFM1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8

UniProt ID: (Human) O95831

Entrez Gene ID: (Human) 9131

分子生物学功能: oxidoreductase

功能

response to ischemia DNA catabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process response to toxic substance chromosome condensation positive regulation of apoptotic process positive regulation of neuron apoptotic process cell redox homeostasis neuron apoptotic process oxidation-reduction process intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide cellular response to estradiol stimulus cellular response to nitric oxide cellular response to oxygen-glucose deprivation response to L-glutamate regulation of apoptotic DNA fragmentation cellular response to aldosterone

参与通路

DNA binding protein binding electron carrier activity NAD(P)H oxidase activity oxidoreductase activity, acting on NAD(P)H protein dimerization activity FAD binding

AIF Monoclonal Antibody (7F7AB10)

产品信息

45-6200

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

种属反应

宿主/亚型

分类

类型

克隆号

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

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