Invitrogen

AIF Monoclonal Antibody (7F7AB10)

Advanced Verification

This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.

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Datasheet

Protocols

Tech Support

Datasheet

Protocols

Tech Support

Antibody Testing Data (8)
Advanced Verification (1)

FIGURE: 1 / 9

AIF Antibody (45-6200) in WB

Western blot was performed using Anti-AIF Monoclonal Antibody (7F7AB10)(Product # 45-6200) and a 60kDa band corresponding to AIF was observed across cell lines tested. Whole cell extracts (30 µg lysate) of K-562 (Lane 1), Hep G2 (Lane 2), MCF7 (Lane 3), HeLa (Lane 4) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1... View More

Product Image Gallery

AIF Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescence analysis of AIF was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with AIF Monoclonal Antibody (7F7AB10) (Product # 45-6200) at 5 µg in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Mouse IgG (H+L) Superclonal™ Recombinant Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175), (1:2000 dilution), for 45 minutes at room temperature (Panel a: Green). Nuclei (Panel b:Blue) were stained with ProLong™ Diamond Antifade Mountant with DAPI (Product # P36962). F-actin (Panel c: Red) was stained with Rhodamine Phalloidin (Product # R415, 1:300 dilution). Panel d represents the merged image showing mitochondria localization. Panel e represents control cells with no primary antibody to assess background. The images were captured at 60x magnification.

AIF Antibody in Flow Cytometry (Flow) — Flow cytometric analysis of AIF in HL-60 cells using an AIF Monoclonal antibody (Product # 45-6200) at 1 µg/mL, as shown in blue. Isotype control antibody is shown in red.

AIF Antibody — Antibody specificity was demonstrated by siRNA mediated knockdown of target protein. HeLa cells were transfected with AIF siRNA and decrease in signal intensity was observed in Western Blot application using Anti-AIF Monoclonal Antibody (7F7AB10) (Product # 45-6200). {KD}

Product Details

45-6200

Applications

Tested Dilution

Publications

免疫印迹 (WB)

2-5 µg/mL

免疫组化 (IHC)

10 µg/mL

免疫细胞化学 (ICC/IF)

5-10 µg/mL

流式细胞分析 (Flow)

1 µg/mL

产品规格

Species Reactivity

Human

Host/Isotype

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

7F7AB10

Immunogen

Human heart mitochondria

View immunogen

Conjugate

Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

IgG fraction

保存液

HEPES buffered saline

内含物

0.02% sodium azide

保存条件

4° C, do not freeze

RRID

AB_2533822

Product Specific Information

When performing IHC use heat mediated antigen retrieval before commencing with IHC staining protocol.

When perfoming ICC or IF recommended sample treatment are paraformaldehyde fixed at 4% for 20 minutes and Triton X-100 permeabilized at 0.1% for 15 minutes.

Target Information

Apoptosis Inducing Factor (AIF) causes chromatin condensation and DNA fragmentation. AIF was recently identified, and cloned. Apoptosis is characterized by several morphological nuclear changes including chromatin condensation and nuclear fragmentation. These changes are triggered by the activation of members of caspase family, caspase activated DNase, and several novel proteins. Like the critical molecules, cytochrome c and caspase-9, in apoptosis, AIF localizes in mitochondria. AIF translocates to the nucleus when apoptosis is induced and induces mitochondria to release the apoptogenic proteins cytochrome c and caspase-9. AIF induces chromatin condensation and large scale DNA fragmentation, which are the hallmarks of apoptosis, of the isolated nucleus and the nucleus in live cells by microinjection and apoptosis stimuli. AIF is highly conserved between human and mouse and widely expressed. Mutations in the AIF gene cause combined oxidative phosphorylation deficiency 6, which results in a severe mitochondrial encephalomyopathy. Alternative splicing results in multiple transcript variants of AIF and a related pseudogene has been identified on chromosome 10.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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Bioinformatics

Protein Aliases: AGR1; Apoptosis-inducing factor 1, mitochondrial; apoptosis-inducing factor, mitochondrion-associated, 1; ERP18; ERP19; hAG-1; MGC111425; pcd8; PDIA16; programmed cell death 8 (apoptosis-inducing factor); Programmed cell death protein 8; RP3-438D16.2; striatal apoptosis-inducing factor; testicular secretory protein Li 4; TLP19; UNQ713/PRO1376

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Gene Aliases: AIF; AIFM1; CMT2D; CMTX4; COWCK; COXPD6; DFNX5; NADMR; NAMSD; PDCD8

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UniProt ID: (Human) O95831

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Entrez Gene ID: (Human) 9131

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Molecular Function: oxidoreductase

Function(s)

response to ischemia DNA catabolic process apoptotic process activation of cysteine-type endopeptidase activity involved in apoptotic process response to toxic substance chromosome condensation positive regulation of apoptotic process positive regulation of neuron apoptotic process cell redox homeostasis neuron apoptotic process oxidation-reduction process intrinsic apoptotic signaling pathway in response to endoplasmic reticulum stress cellular response to hydrogen peroxide cellular response to estradiol stimulus cellular response to nitric oxide cellular response to oxygen-glucose deprivation response to L-glutamate regulation of apoptotic DNA fragmentation cellular response to aldosterone

Process(es)

DNA binding protein binding electron carrier activity NAD(P)H oxidase activity oxidoreductase activity, acting on NAD(P)H protein dimerization activity FAD binding

Performance Guarantee

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AIF Monoclonal Antibody (7F7AB10)

AIF Antibody (45-6200) in WB

Product Details

45-6200

免疫印迹 (WB)

免疫组化 (IHC)

免疫细胞化学 (ICC/IF)

流式细胞分析 (Flow)

Species Reactivity

Host/Isotype

分类

类型

克隆号

Immunogen

Conjugate

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

Product Specific Information

Target Information

References (0)

Bioinformatics

Performance Guarantee

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