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          • Primary Antibodies ›
          • AKT Pan Antibodies

          Invitrogen

          AKT Pan Polyclonal Antibody

          2 篇参考文献
          查看其他33个AKT Pan抗体

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          Cite AKT Pan Polyclonal Antibody

          Additional Information:
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          • 抗体检测数据 (7)
          AKT Pan Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          AKT Pan Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 7

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          AKT Pan Antibody (PA5-77855) in ICC/IF

          Immunocytochemistry-Immunofluorescence analysis of AKT Pan was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: AKT Pan Polyclonal Antibody (Product # PA5 77855) diluted at 1:500. Blue: Hoechst 33342 staining. Scale bar = 10 µm. {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          AKT Pan Antibody in Immunocytochemistry (ICC/IF)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Western Blot (WB)
          AKT Pan Antibody in Immunoprecipitation (IP)

          Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

          产品信息

          PA5-77855

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:500-1:3,000
          查看2篇已发表文章 2篇已发表文章

          免疫组化(石蜡) (IHC (P))

          1:100-1:500
          -

          免疫组化(冰冻) (IHC (F))

          1:100-1:500
          -

          免疫细胞化学 (ICC/IF)

          1:100-1:1,000
          -

          免疫沉淀 (IP)

          1:100-1:500
          -
          产品规格

          种属反应

          Fish, Human, Mouse, Pig, Rat

          已发表种属

          Human, Rat

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          Recombinant protein encompassing a sequence within the center region of human Akt1/2/3. The exact sequence is proprietary.

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          0.79 mg/mL

          规格

          79 µg

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS, pH 7, with 20% glycerol

          内含物

          0.025% ProClin 300

          保存条件

          Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2736315

          产品详细信息

          Positive Control: 293T, JC, BCL-1, PC-12, mouse melanoma

          Predicted Reactivity: Zebrafish (93%), Xenopus laevis (99%), Cat (98%), Pig (99%), Chicken (100%), Sheep (99%), Rhesus Monkey (100%), Bovine (99%)

          Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

          靶标信息

          AKT also known as protein kinase B (PKB) or RAS-alpha, is an ubiquitous serine/threonine kinase that plays an important role in diverse biological responses such as regulation of metabolism, cell survival and growth by phosphorylating multiple proteins. This protein kinase is activated by insulin, PI3K, IGF1 and various other growth and survival factors. Akt promotes cell survival by inhibiting apoptosis through phosphorylation and inactivation of several targets, including forkhead transcription factors, and caspase-9. The AKT pathway is a major target for cancer drug discovery.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: AKT1 kinase; AKT1m; Akt1m protein; Akt2; AKT2 kinase; AKT3 kinase; DKFZp434N0250; kinase Akt1; MGC99656; murine thymoma viral (v-akt) homolog-2; murine thymoma viral (v-akt) oncogene homolog 1; murine thymoma viral (v-akt) oncogene homolog 2; pan akt; PKB alpha; PKB beta; PKB gamma; PKBgamma/Akt3; PKBgamma1; protein kinase Akt-2; protein kinase Akt-3; protein kinase B; protein kinase B alpha; protein kinase B beta; protein kinase B gamma; protein kinase B, beta; protein kinase B, gamma; protein kinase B-alpha; proto-oncogene c-AKT; proto-oncogene product; putative v-akt murine thymoma viral oncoprotein 2; rac protein kinase alpha; RAC protein kinase alpha RAC-PK alpha; rac protein kinase beta; RAC protein kinase beta RAC-PK beta; RAC-gamma serine/threonine protein kinase; RAC-PK-alpha; RAC-PK-beta; RAC-PK-gamma; related to A and C kinases; serine-threonine protein kinase; similar to rat serine-threonine protein kinase; thymoma viral proto-oncogene 1; thymoma viral proto-oncogene 2; thymoma viral proto-oncogene 3; tumor rejection antigen peptide pRLib; unnamed protein product; v-akt murine thymoma viral oncogene 3; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene homolog 2; v-akt murine thymoma viral oncogene homolog 3 (protein kinase B, gamma); v-akt murine thymoma viral oncogene-like 1; v-akt murine thymoma viral oncogene-like 3; v-akt murine thymoma viral oncogene-like protein 1

          查看更多 收起

          基因别名: 2410016A19Rik; AKT; D930002M15Rik; HIHGHH; LTR-akt; MPPH; MPPH2; Nmf350; PKB; PKB-ALPHA; PKB-GAMMA; PKB/Akt; PKBalpha; PKBB; PKBBETA; PKBG; PKBgamma; PRKBA; PRKBB; PRKBG; RAC; RAC-ALPHA; RAC-BETA; RAC-gamma; RAC-PK-gamma; STK-2

          查看更多 收起

          UniProt ID: (Human) P31749, (Human) P31751, (Human) Q9Y243, (Rat) P47196, (Mouse) P31750, (Mouse) Q60823, (Rat) P47197, (Mouse) Q9WUA6, (Rat) Q63484

          查看更多 收起

          Entrez Gene ID: (Human) 207, (Pig) 100126861, (Human) 208, (Pig) 100127478, (Human) 10000, (Pig) 100513811, (Rat) 24185, (Mouse) 11651, (Mouse) 11652, (Rat) 25233, (Mouse) 23797, (Rat) 29414

          查看更多 收起

          功能
          protein kinase activity protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding calmodulin binding ATP binding phosphatidylinositol-3,4,5-trisphosphate binding kinase activity enzyme binding kinase binding protein kinase binding nitric-oxide synthase regulator activity protein serine/threonine kinase inhibitor activity identical protein binding protein homodimerization activity phosphatidylinositol-3,4-bisphosphate binding 14-3-3 protein binding potassium channel activator activity protein serine kinase activity TORC2 complex binding molecular function activator activity protein kinase C binding GTPase activating protein binding protein phosphatase 2A binding protein serine/threonine kinase binding non-receptor serine/threonine protein kinase protein modifying enzyme
          参与通路
          osteoblast differentiation positive regulation of endothelial cell proliferation cell migration involved in sprouting angiogenesis complement receptor mediated signaling pathway sphingosine-1-phosphate signaling pathway regulation of glycogen biosynthetic process protein phosphorylation nitric oxide biosynthetic process activation-induced cell death of T cells response to oxidative stress signal transduction epidermal growth factor receptor signaling pathway G-protein coupled receptor signaling pathway cell proliferation insulin receptor signaling pathway response to heat response to hormone negative regulation of autophagy positive regulation of endothelial cell migration positive regulation of gene expression negative regulation of gene expression negative regulation of plasma membrane long-chain fatty acid transport fibroblast migration positive regulation of glucose metabolic process regulation of neuron projection development negative regulation of macroautophagy peptidyl-serine phosphorylation peptidyl-threonine phosphorylation cytokine-mediated signaling pathway cell differentiation positive regulation of cell growth regulation of cell migration positive regulation of cell migration T cell costimulation negative regulation of protein ubiquitination TOR signaling negative regulation of fatty acid beta-oxidation positive regulation of endodeoxyribonuclease activity positive regulation of proteasomal ubiquitin-dependent protein catabolic process cellular response to insulin stimulus positive regulation of peptidyl-serine phosphorylation cellular response to stress response to fluid shear stress intracellular signal transduction interleukin-18-mediated signaling pathway positive regulation of protein import into nucleus regulation of apoptotic process negative regulation of apoptotic process anoikis regulation of mRNA stability protein kinase B signaling positive regulation of blood vessel endothelial cell migration positive regulation of nitric oxide biosynthetic process positive regulation of fat cell differentiation positive regulation of glycogen biosynthetic process negative regulation of Notch signaling pathway negative regulation of proteolysis positive regulation of transcription from RNA polymerase II promoter nitric oxide metabolic process positive regulation of glucose import positive regulation of lipid biosynthetic process insulin-like growth factor receptor signaling pathway behavioral response to pain positive regulation of smooth muscle cell proliferation positive regulation of protein metabolic process positive regulation of protein kinase B signaling excitatory postsynaptic potential response to growth hormone mammary gland epithelial cell differentiation response to UV-A protein localization to mitochondrion response to growth factor cellular response to epidermal growth factor stimulus maintenance of protein location in mitochondrion cellular response to rapamycin negative regulation of release of cytochrome c from mitochondria vascular endothelial cell response to laminar fluid shear stress regulation of postsynapse organization regulation of tRNA methylation cellular response to oxidised low-density lipoprotein particle stimulus negative regulation of protein localization to lysosome negative regulation of cGAS/STING signaling pathway beta-arrestin-dependent dopamine receptor signaling pathway positive regulation of G1/S transition of mitotic cell cycle positive regulation of protein localization to nucleus regulation of signal transduction by p53 class mediator negative regulation of cilium assembly negative regulation of oxidative stress-induced intrinsic apoptotic signaling pathway negative regulation of leukocyte cell-cell adhesion positive regulation of protein localization to plasma membrane negative regulation of protein maturation negative regulation of hydrogen peroxide-induced neuron intrinsic apoptotic signaling pathway negative regulation of PERK-mediated unfolded protein response positive regulation of TORC1 signaling positive regulation of TORC2 signaling positive regulation of protein localization to endoplasmic reticulum positive regulation of anaphase-promoting complex-dependent catabolic process cellular response to nerve growth factor stimulus response to insulin-like growth factor stimulus positive regulation of protein localization to cell surface regulation of type B pancreatic cell development negative regulation of lymphocyte migration negative regulation of extrinsic apoptotic signaling pathway in absence of ligand negative regulation of intrinsic apoptotic signaling pathway positive regulation of fatty acid beta-oxidation protein modification process fat cell differentiation protein stabilization regulation of cell cycle positive regulation of protein targeting to membrane positive regulation of cap-dependent translational initiation positive regulation of cell motility regulation of mitochondrion organization positive regulation of angiogenesis positive regulation of cell migration involved in sprouting angiogenesis positive regulation of vascular endothelial cell proliferation positive regulation of artery morphogenesis negative regulation of cellular senescence maternal placenta development response to ischemia glycogen metabolic process glycogen biosynthetic process glucose metabolic process translation inflammatory response cellular response to DNA damage stimulus smoothened signaling pathway germ cell development apoptotic mitochondrial changes gene expression positive regulation of fibroblast migration positive regulation of sodium ion transport positive regulation of mitochondrial membrane potential response to muscle activity protein ubiquitination spinal cord development lateral motor column neuron differentiation oogenesis stage cell projection organization protein catabolic process regulation of myelination lipopolysaccharide-mediated signaling pathway response to nutrient levels response to food peripheral nervous system myelin maintenance regulation of protein localization negative regulation of superoxide anion generation cellular response to heat cellular response to vascular endothelial growth factor stimulus cellular response to platelet-derived growth factor stimulus cellular response to decreased oxygen levels glucose homeostasis positive regulation of apoptotic process negative regulation of cell size positive regulation of vasoconstriction negative regulation of JNK cascade positive regulation of organ growth protein autophosphorylation striated muscle cell differentiation glycogen cell differentiation involved in embryonic placenta development labyrinthine layer blood vessel development cellular response to mechanical stimulus cellular response to tumor necrosis factor cellular response to growth factor stimulus cellular response to prostaglandin E stimulus cellular response to hypoxia cellular hyperosmotic salinity response cellular response to sorbitol cellular response to granulocyte macrophage colony-stimulating factor stimulus execution phase of apoptosis negative regulation of calcium import into the mitochondrion cellular response to peptide regulation of aerobic respiration cytoplasmic translation gluconeogenesis translational initiation ubiquitin-dependent protein catabolic process protein import into nucleus NLS-bearing protein import into nucleus ER to Golgi vesicle-mediated transport Golgi to plasma membrane transport cytoskeleton organization cellular response to starvation phosphorylation cell migration negative regulation of cell migration anaphase-promoting complex-dependent catabolic process SCF-dependent proteasomal ubiquitin-dependent protein catabolic process cellular response to nutrient levels negative regulation of toll-like receptor signaling pathway positive regulation of toll-like receptor signaling pathway toll-like receptor 4 signaling pathway cellular response to amino acid starvation protein K29-linked ubiquitination SREBP-SCAP complex retention in endoplasmic reticulum tRNA stabilization calcium ion transmembrane import into mitochondrion PERK-mediated unfolded protein response TORC1 signaling Golgi to plasma membrane protein transport proteasome-mediated ubiquitin-dependent protein catabolic process positive regulation of cholesterol biosynthetic process positive regulation of cilium assembly negative regulation of translational initiation positive regulation of translational initiation protein maturation regulation of vesicle-mediated transport protein localization to cilium protein localization to endoplasmic reticulum protein K11-linked ubiquitination cellular response to calcium ion tricarboxylic acid metabolic process protein K6-linked ubiquitination tRNA (guanine-N7)-methylation cGAS/STING signaling pathway negative regulation of TORC1 signaling positive regulation of protein phosphorylation positive regulation of signal transduction response to insulin negative regulation of RNA splicing positive regulation of positive chemotaxis cellular response to high light intensity protein localization to plasma membrane activation of GTPase activity retinal rod cell apoptotic process response to hypoxia cellular response to hormone stimulus positive regulation of adipose tissue development positive regulation of TOR signaling positive regulation of cell size brain morphogenesis homeostasis of number of cells within a tissue
          It has to be done as per old AB suggested Products section.

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          Host server : magellan-search-blue-76c4fffd86-kv5sr:80/100.66.77.199:80.
          git-commit: bdc067a1e9e92d056899a2dda64af631988aa8a1
          git-url: https://github.com/thermofisher/magellan-search
          git-branch: release/2.54.0-Offline