Invitrogen

ALY Polyclonal Antibody

图: 1 / 7

ALY Antibody (PA5-96489) in WB

Western blot analysis of ALY in extracts of various cell lines. Samples were incubated with ALY Polyclonal antibody (Product # PA5-96489) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Product Image Gallery

ALY Antibody in Immunocytochemistry (ICC/IF) — Confocal immunofluorescence (Immunocytochemistry) analysis of ALY in U2OS cells. Samples were incubated with ALY Polyclonal antibody (Product # PA5-96489) using a dilution of 1:100. Blue: DAPI for nuclear staining.

ALY Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of ALY in paraffin-embedded mouse liver. Samples were incubated with ALY Polyclonal antibody (Product # PA5-96489) using a dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.

产品信息

PA5-96489

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫组化（石蜡） (IHC (P))

1:50-1:200

免疫细胞化学 (ICC/IF)

1:50-1:200

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthetic peptide corresponding to amino acids 1-100 of human ALY.

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

1.48 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.01% thimerosal

保存条件

-20° C, Avoid Freeze/Thaw Cycles

RRID

AB_2808291

产品详细信息

Positive Samples: HeLa, A-549, B cells, SKOV3, HL-60, Mouse testis, Mouse spleen, Mouse thymus, Rat brain; Cellular Location: Cytoplasm, Nucleus, Nucleus speckle

靶标信息

The protein encoded by this gene is a heat stable, nuclear protein and functions as a molecular chaperone. It is thought to regulate dimerization, DNA binding, and transcriptional activity of basic region-leucine zipper (bZIP) proteins.

⚠WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause birth defects or other reproductive harm. For more information go to www.P65Warnings.ca.gov.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Ally of AML-1 and LEF-1; Aly/REF export factor; bZIP enhancing factor; bZIP-enhancing factor BEF; REF1-I; RNA and export factor binding protein 1; RNA and export factor-binding protein 1; Tcra enhancer-binding factor interacting protein 1; THO complex 4; THO complex subunit 4; Tho4; Transcriptional coactivator Aly/REF

基因别名: ALY; ALY/REF; ALYREF; BEF; REF; REF1; Refbp1; THOC4

UniProt ID: (Human) O43672, (Mouse) O08583

Entrez Gene ID: (Human) 10189, (Rat) 690585, (Mouse) 21681

分子生物学功能: RNA metabolism protein