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Active Motif
AM-Tag Recombinant Mouse Monoclonal Antibody, AbFlex®
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AM-Tag Antibody (91111) in WB
AbFlex™ AM-Tag antibody (rAb) tested by Western Blot. Active Motif’s pAM_1C_JunD Vector (Catalog No. 53044) was transfected, mock transfected or transfected with pAM_1C Empty Vector (Catalog No. 53023) into HeLa or HCT116 cells using 10 µg DNA and 30 µl FuGENE transfection reagent, 48 hours post-transfection nuclear lysates were prepared. 20 µg lysate was loaded per well. Western blot was performed using a 1:500 dilution of AM-Tag antibody.
产品信息
91111
产品规格
种属反应
Tag
宿主/亚型
Mouse
/ IgG2a
Expression System
HEK293 cells
分类
Recombinant Monoclonal
类型
Antibody
抗原
This antibody was raised against Active Motif's unique AM-Tag sequence.
偶联物
形式
Liquid
浓度
1 mg/mL
纯化类型
Protein A
保存液
140mM HEPES/32mM sodium acetate, pH 7.5, with 30% glycerol, 70mM NaCl
内含物
0.035% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
RRID
靶标信息
The AM-Tag antibody is specifically designed for use with Active Motif's unique AM-Tag sequence in combination with the Tag-ChIP-IT® Kit (Catalog No. 53022). Simply clone your protein of interest into Active Motif's pAM_1C Empty Vector (Catalog No. 53023), or add the AM-Tag sequence to the C-terminus of your protein expression vector. Following transfection, cell lysates are prepared and screened by Western blot for expression of the AM-Tag fusion protein using the AM-Tag antibody. Optimization may be required to identify the optimal conditions for transfection and protein expression.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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