Invitrogen

APE1 Polyclonal Antibody

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1 篇参考文献
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说明书
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说明书
实验方案
问题与答案
技术支持

产品信息

PA5-29157

应用
建议稀释比
已发表文章

免疫印迹 (WB)

1:500-1:20,000
-

免疫组化（石蜡） (IHC (P))

1:100-1:1,000
-

免疫细胞化学 (ICC/IF)

1:100-1:1,000
查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

1:100-1:500
-

染色质免疫沉淀 实验 (ChIP)

1.5 µg/10^6 cells
-
产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant protein encompassing a sequence within the center region of human APE1. The exact sequence is proprietary.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.34 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_2546633

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, mouse brain, PC-12.

Predicted reactivity: Mouse (94%), Rat (93%), Dog (95%), Pig (94%), Rhesus Monkey (98%), Chimpanzee (99%), Bovine (93%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Mammalian apurinic/apyrimidinic endonuclease (APE/ref-1) is a multifunctional, bipartite enzyme that plays an important role in numerous, cellular functions. APE is responsible for repairing abasic sites in DNA and in regulating the redox state of other proteins that play roles in oxidative signaling, transcription factor regulation (Fos, Jun, NF-kB, Myb, HIF-1 alpha, CREB, Pax), cell cycle control (p53), and apoptosis. The most common form of DNA damage is the creation of abasic sites which are brought about through spontaneous loss or oxidative DNA damage, through chemically initiated hydrolysis (chemotherapy), ionizing radiation, UV irradiation, oxidizing agents, and removal of modified bases by DNA glycosylases. APE is differentially expressed during development and in different tissues. This protein has diverse subcellular localization patterns which support the possibility of its interaction with numerous, other cellular proteins in addition to DNA repair within the nucleus. Regulation of APE by phosphorylation is mediated, at least in part, by casein kinase II. Increases in APE message and protein levels are observed upon the reintroduction of oxygen to hypoxic cells, and in some malignant tissue relative to normal tissue. Decreases in APE expression have been associated with the induction of cellular apoptosis.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: AP endonuclease 1; AP endonuclease class I; AP lyase; APEN; APEX nuclease; APEX nuclease (multifunctional DNA repair enzyme) 1; APEX nuclease 1; Apurinic-apyrimidinic endonuclease 1; apurinic/a; Apurinic/apy; apurinic/apyrimidinic (abasic) endonuclease; apurinic/apyrimidinic endonuclease 1; deoxyribonuclease (apurinic or apyrimidinic); DNA repair nuclease/redox regulator APEX1; DNA-(apurinic or apyrimidinic site) endonuclease; DNA-(apurinic or apyrimidinic site) lyase; protein REF-1; redox factor 1; Redox factor-1; REF-1

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基因别名: APE; APE1; APEN; APEX; APEX1; APX; HAP1; REF-1; REF1

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UniProt ID: (Human) P27695, (Mouse) P28352, (Rat) P43138

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Entrez Gene ID: (Human) 328, (Mouse) 11792, (Rat) 79116

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功能
DNA binding damaged DNA binding double-stranded telomeric DNA binding transcription coactivator activity transcription corepressor activity DNA-(apurinic or apyrimidinic site) lyase activity endonuclease activity endodeoxyribonuclease activity RNA-DNA hybrid ribonuclease activity phosphodiesterase I activity uracil DNA N-glycosylase activity protein binding phosphoric diester hydrolase activity double-stranded DNA exodeoxyribonuclease activity double-stranded DNA 3'-5' exodeoxyribonuclease activity 3'-5' exonuclease activity oxidoreductase activity site-specific endodeoxyribonuclease activity, specific for altered base chromatin DNA binding protein complex binding poly(A) RNA binding metal ion binding NF-kappaB binding RNA binding nuclease activity endoribonuclease activity exonuclease activity hydrolase activity lyase activity phosphoric ester hydrolase activity T/G mismatch-specific endonuclease activity retroviral 3' processing activity
参与通路
DNA repair base-excision repair DNA recombination transcription, DNA-templated regulation of transcription, DNA-templated cellular response to DNA damage stimulus aging negative regulation of smooth muscle cell migration regulation of mRNA stability cell redox homeostasis positive regulation of DNA repair oxidation-reduction process cellular response to hydrogen peroxide DNA demethylation nucleic acid phosphodiester bond hydrolysis RNA phosphodiester bond hydrolysis, endonucleolytic positive regulation of G1/S transition of mitotic cell cycle regulation of nucleic acid-templated transcription telomere maintenance base-excision repair, base-free sugar-phosphate removal response to drug cellular response to cAMP cellular response to peptide hormone stimulus negative regulation of nucleic acid-templated transcription DNA catabolic process, endonucleolytic DNA catabolic process, exonucleolytic response to organonitrogen compound cellular response to organonitrogen compound
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