Invitrogen

APOE Recombinant Rabbit Monoclonal Antibody (16H22L18)

APOE Antibody in Immunocytochemistry (ICC/IF)

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APOE Antibody (701241) in ICC/IF

Immunofluorescence analysis of APOE was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 45 minutes at room temperature. The cells were labeled with APOE Recombinant Rabbit Monoclonal Antibody (16H22L18) (Product # 701241) at 1:100 dilution in 0.1% BSA, incubated at 4 degree celsius overnight and then labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Recombinant Secondary Antibody, ... View More

APOE Antibody in Immunohistochemistry (Frozen), Proximity Ligation Assay (PLA) (IHC (F), PLA)

APOE Antibody in Immunohistochemistry, Immunohistochemistry (Frozen) (IHC, IHC (F))

APOE Antibody in Immunohistochemistry, Proximity Ligation Assay (PLA) (IHC, PLA)

APOE Antibody in Western Blot, Immunoprecipitation (WB, IP)

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APOE Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Relative expression of APOE was observed in Hep G2, CaCo-2 as compared to A-431, Daudi using Anti-APOE Recombinant Rabbit Monoclonal Antibody (16H22L18) (Product # 701241) in Western Blot. {RE}

产品信息

701241

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2-4 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫组化（冰冻） (IHC (F))

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免疫细胞化学 (ICC/IF)

1-3 µg/mL

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免疫沉淀 (IP)

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in situ PLA (PLA)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

16H22L18

抗原

Peptide corresponding to amino acids 240-251 of human ApoE

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

AB_2532438

产品详细信息

This antibody is predicted to react with non-human primate and mouse based on sequence homology.

Intact IgG appears on a non-reducing gel as ~150 kDa band and upon reduction generating a ~25 kDa light chain band and a ~50 kDa heavy chain.

Recombinant rabbit monoclonal antibodies are produced using in vitro expression systems. The expression systems are developed by cloning in the specific antibody DNA sequences from immunoreactive rabbits. Then, individual clones are screened to select the best candidates for production. The advantages of using recombinant rabbit monoclonal antibodies include: better specificity and sensitivity, lot-to-lot consistency, animal origin-free formulations, and broader immunoreactivity to diverse targets due to larger rabbit immune repertoire.

靶标信息

Apo E (Apolipoprotein E) plays an important role in the metabolism of lipids in the plasma, and is also is a constituent of various plasma lipoprotein-lipid particles. The protein is synthesized in liver, brain, spleen, and the kidney, and concentrations are high in interstitial fluids, where it plays a role in distributing cholesterol from cells where cholesterol is in excess to cells which require cholesterol. Apo E also plays an important role in the formation of very low density lipoprotein and chylomicrons. The Apo E gene is located on chromosome 19 at position 19q13. 2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

A semi-automated pipeline for quantifying drusen-like deposits in human induced pluripotent stem cell-derived retinal pigment epithelium cells.

SLAS technology

Hall J,Daniszewski M,Cheung S,Shobhana K,Kumar H,Liang HH,Beetham H,Cho E,Abbott C,Hewitt AW,Simpson KJ,Guymer RH,Paull D,Pébay A,Lidgerwood GE

701241 was used in Immunocytochemistry to develop an imaging and analysis pipeline that quantifies the number of drusen-like deposits, and accurately and reproducibly provides the location and composition of these deposits.

Sat Jun 01 00:00:00 UTC 2024

Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation.

F1000Research

Ayoubi R,Southern K,Laflamme C

Published figure using APOE recombinant monoclonal antibody (Product # 701241) in Western Blot

Wed Jan 03 00:00:00 UTC 2024

Identification of high-performing antibodies for Apolipoprotein E for use in Western Blot and immunoprecipitation.

F1000Research

Ayoubi R,Southern K,Laflamme C

Published figure using APOE recombinant monoclonal antibody (Product # 701241) in Western Blot

Wed Jan 03 00:00:00 UTC 2024

Enhanced liver X receptor signalling reduces brain injury and promotes tissue regeneration following experimental intracerebral haemorrhage: roles of microglia/macrophages.

Stroke and vascular neurology

Zhang R,Dong Y,Liu Y,Moezzi D,Ghorbani S,Mirzaei R,Lozinski BM,Dunn JF,Yong VW,Xue M

701241 was used in Immunohistochemistry (Frozen) to examine the benefits of enhanced Liver X receptor signaling in experimental intracerebral hemorrhage.

Fri Dec 29 00:00:00 UTC 2023

C/EBPβ/AEP signaling couples atherosclerosis to the pathogenesis of Alzheimer's disease.

Molecular psychiatry

Liao J,Chen G,Liu X,Wei ZZ,Yu SP,Chen Q,Ye K

701241 was used in Immunohistochemistry to demonstrate that C/EBPβ/AEP signaling couples ATH to AD via mediating vascular pathology.

Fri Jul 01 00:00:00 UTC 2022

Monomeric C-reactive protein via endothelial CD31 for neurovascular inflammation in an ApoE genotype-dependent pattern: A risk factor for Alzheimer's disease?

Aging cell

Zhang Z,Na H,Gan Q,Tao Q,Alekseyev Y,Hu J,Yan Z,Yang JB,Tian H,Zhu S,Li Q,Rajab IM,Blusztajn JK,Wolozin B,Emili A,Zhang X,Stein T,Potempa LA,Qiu WQ