Invitrogen

APP (Amyloid Precursor Protein) Monoclonal Antibody (22C11), eBioscience™

APP (Amyloid Precursor Protein) Antibody in Immunocytochemistry (ICC/IF)

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APP (Amyloid Precursor Protein) Antibody (14-9749-82) in ICC/IF

Immunocytochemistry of MeOH-fixed SK-N-SH cells using 2.5 µg/mL of Mouse IgG1 K Isotype Control Purified (left) or 2.5 µg/mL of Anti-APP (Amyloid Precursor Protein) Purified (right) followed by F(ab')2 Anti-Mouse IgG eFluor® 570. Nuclei are stained with DAPI.

APP (Amyloid Precursor Protein) Antibody in Western Blot (WB)

APP (Amyloid Precursor Protein) Antibody in Immunohistochemistry (IHC)

APP (Amyloid Precursor Protein) Antibody

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产品信息

14-9749-82

应用

建议稀释比

已发表文章

免疫印迹 (WB)

2 µg/mL

查看8篇已发表文章 8篇已发表文章

免疫组化 (IHC)

查看3篇已发表文章 3篇已发表文章

免疫组化（冰冻） (IHC (F))

Assay-dependent

免疫细胞化学 (ICC/IF)

2.5 µg/mL

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

22C11

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2572978

产品详细信息

Description: The monoclonal antibody 22C11 recognizes human, mouse, and rat APP (Amyloid Precursor Protein). APP is expressed in high abundance in the central nervous system and has three major isoforms resulting from alternative splicing. APP plays a role in synaptic formation and repair, anterograde neuronal transport, iron export, and hormonal regulation. Secreted APP (sAPP) may have neuroprotective effects against neurotoxic insult, oxidative stress, and excitotoxicity. APP belongs to a family that contains at least two homologs, amyloid precursor-like proteins 1 and 2 (APLP1 and APLP2). Similarities between APP and APLP, especially APLP2, suggest that APLP could share and compensate for the function of APP. Proteolytic cleavage of APP results in the generation of beta amyloid, which is the primary component of senile plaques. Senile plaques are one of the major histopathologic features of Alzheimer's disease. Abnormal regulation and processing of APP also plays a role in Down's syndrome, early onset familial Alzheimer's disease, cerebral hemorrhage, and arthritis.

This 22C11 antibody reacts with pre-A4 and recognizes all three isoforms of APP (immature, sAPP, and mature). This 22C11 antibody is known to cross react with APLP2.

Applications Reported: This 22C11 antibody has been reported for use in microscopy, and immunocytochemistry.

Applications Tested: This 22C11 antibody has been tested by immunocytochemistry of methanol-fixed cells and can be used at less than or equal to 2.5 µg/mL. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Purity: Greater than 90%, as determined by SDS-PAGE.

Aggregation: Less than 10%, as determined by HPLC.

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

Amyloid Precursor Protein (APP) or Amyloid beta precursor protein functions as a cell surface kinesin I membrane receptor, mediating the axonal transport of beta-secretase and presenilin 1. APP is important for neurite growth, neuronal adhesion and axonogenesis. APP is a 100-140 kDa transmembrane glycoprotein that exists as several isoforms resulting from alternative splicing. Proteolytic cleavage of APP by beta- and gamma-secretases results in the generation of beta amyloid, which is the primary component of senile plaques. Senile plaques are one of the major histopathologic features of Alzheimer's disease. Abnormal regulation and processing of APP also plays a role in Down's syndrome, early onset familial Alzheimer's disease, and cerebral hemorrhage.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Melanoma-Secreted Amyloid Beta Suppresses Neuroinflammation and Promotes Brain Metastasis.

Cancer discovery

Kleffman K,Levinson G,Rose IVL,Blumenberg LM,Shadaloey SAA,Dhabaria A,Wong E,Galán-Echevarría F,Karz A,Argibay D,Von Itter R,Floristán A,Baptiste G,Eskow NM,Tranos JA,Chen J,Vega Y Saenz de Miera EC,Call M,Rogers R,Jour G,Wadghiri YZ,Osman I,Li YM,Mathews P,DeMattos RB,Ueberheide B,Ruggles KV,Liddelow SA,Schneider RJ,Hernando E

14-9749-82 was used in Western Blotting to reveal a novel mechanistic connection between brain metastasis and Alzheimer's disease, two previously unrelated pathologies; establish Aβ as a promising therapeutic target for brain metastasis; and demonstrate suppression of neuroinflammation as a critical feature of metastatic adaptation to the brain parenchyma.

Mon May 02 00:00:00 UTC 2022

The Potential Neuroprotective Effects of Extracts from Oat Seedlings against Alzheimer's Disease.

Nutrients

Lee WS,Lee HJ,Yang JY,Shin HL,Choi SW,Kim JK,Seo WD,Kim EH

Published figure using APP (Amyloid Precursor Protein) monoclonal antibody (Product # 14-9749-82) in Western Blot

Sun Oct 02 00:00:00 UTC 2022

The Potential Neuroprotective Effects of Extracts from Oat Seedlings against Alzheimer's Disease.

Nutrients

Lee WS,Lee HJ,Yang JY,Shin HL,Choi SW,Kim JK,Seo WD,Kim EH

Published figure using APP (Amyloid Precursor Protein) monoclonal antibody (Product # 14-9749-82) in Western Blot

Sun Oct 02 00:00:00 UTC 2022

Beta-Site Amyloid Precursor Protein-Cleaving Enzyme Inhibition Partly Restores Sevoflurane-Induced Deficits on Synaptic Plasticity and Spine Loss.

International journal of molecular sciences

Wang X,Shi Q,Pradhan AK,Ziegon L,Schlegel M,Rammes G

14-9749-82 was used in Western Blotting and Immunohistochemistry to suggest that sevo partly elevates BACE activity and interferes with synaptic remodeling, whereas iso mildly modulates synaptic changes in the CA1 region of the hippocampus.

Tue Jun 14 00:00:00 UTC 2022

Beta-Site Amyloid Precursor Protein-Cleaving Enzyme Inhibition Partly Restores Sevoflurane-Induced Deficits on Synaptic Plasticity and Spine Loss.

International journal of molecular sciences

Wang X,Shi Q,Pradhan AK,Ziegon L,Schlegel M,Rammes G

Tue Jun 14 00:00:00 UTC 2022

APP accumulates with presynaptic proteins around amyloid plaques: A role for presynaptic mechanisms in Alzheimer's disease?

Alzheimer's & dementia : the journal of the Alzheimer's Association

Jordà-Siquier T,Petrel M,Kouskoff V,Smailovic U,Cordelières F,Frykman S,Müller U,Mulle C,Barthet G

14-9749-82 was used in Immunohistochemistry-immunofluorescence to report that the prominent somatic distribution of APP observed in control patients remarkably vanishes in human AD patients to the benefit of dense accumulations of extra-somatic APP, which surround dense-core amyloid plaques enriched in APP-Nter.

Tue Nov 01 00:00:00 UTC 2022

Temporal and brain region-specific elevations of soluble Amyloid-β_40-42 in the Ts65Dn mouse model of Down syndrome and Alzheimer's disease.

Aging cell

Tallino S,Winslow W,Bartholomew SK,Velazquez R

14-9749-82 was used in Western Blotting to show that trisomic mice accumulate soluble Aβ40-42 in the basal forebrain, frontal cortex, hippocampus, and cerebellum in an age-specific manner, with elevation in the frontal cortex and hippocampus as early as 4 months of age.

Fri Apr 01 00:00:00 UTC 2022

GSAP regulates lipid homeostasis and mitochondrial function associated with Alzheimer's disease.

The Journal of experimental medicine

Xu P,Chang JC,Zhou X,Wang W,Bamkole M,Wong E,Bettayeb K,Jiang LL,Huang T,Luo W,Xu H,Nairn AC,Flajolet M,Ip NY,Li YM,Greengard P

Published figure using APP (Amyloid Precursor Protein) monoclonal antibody (Product # 14-9749-82) in Western Blot

Mon Aug 02 00:00:00 UTC 2021

GSAP regulates lipid homeostasis and mitochondrial function associated with Alzheimer's disease.

The Journal of experimental medicine

Xu P,Chang JC,Zhou X,Wang W,Bamkole M,Wong E,Bettayeb K,Jiang LL,Huang T,Luo W,Xu H,Nairn AC,Flajolet M,Ip NY,Li YM,Greengard P

Published figure using APP (Amyloid Precursor Protein) monoclonal antibody (Product # 14-9749-82) in Western Blot

Mon Aug 02 00:00:00 UTC 2021

microRNA-425 loss mediates amyloid plaque microenvironment heterogeneity and promotes neurodegenerative pathologies.

Aging cell

Hu YB,Zhang YF,Ren RJ,Dammer EB,Xie XY,Chen SW,Huang Q,Huang WY,Zhang R,Chen HZ,Wang H,Wang G

14-9749-82 was used in Immunocytochemistry, Immunohistochemistry to show that AD-associated pathophysiology is spatially colocalized to amyloid plaques in regions with abnormal microRNA-425 levels.

Fri Oct 01 00:00:00 UTC 2021

microRNA-425 loss mediates amyloid plaque microenvironment heterogeneity and promotes neurodegenerative pathologies.

Aging cell

Hu YB,Zhang YF,Ren RJ,Dammer EB,Xie XY,Chen SW,Huang Q,Huang WY,Zhang R,Chen HZ,Wang H,Wang G

14-9749-82 was used in Immunocytochemistry, Immunohistochemistry to show that AD-associated pathophysiology is spatially colocalized to amyloid plaques in regions with abnormal microRNA-425 levels.

Fri Oct 01 00:00:00 UTC 2021

microRNA-425 loss mediates amyloid plaque microenvironment heterogeneity and promotes neurodegenerative pathologies.

Aging cell

Hu YB,Zhang YF,Ren RJ,Dammer EB,Xie XY,Chen SW,Huang Q,Huang WY,Zhang R,Chen HZ,Wang H,Wang G

14-9749-82 was used in Immunocytochemistry, Immunohistochemistry to show that AD-associated pathophysiology is spatially colocalized to amyloid plaques in regions with abnormal microRNA-425 levels.

Fri Oct 01 00:00:00 UTC 2021

APOE4 exacerbates synapse loss and neurodegeneration in Alzheimer's disease patient iPSC-derived cerebral organoids.

Nature communications

Zhao J,Fu Y,Yamazaki Y,Ren Y,Davis MD,Liu CC,Lu W,Wang X,Chen K,Cherukuri Y,Jia L,Martens YA,Job L,Shue F,Nguyen TT,Younkin SG,Graff-Radford NR,Wszolek ZK,Brafman DA,Asmann YW,Ertekin-Taner N,Kanekiyo T,Bu G

14-9749 was used in Western Blotting to suggest APOE4-related degenerative pathways contributing to AD pathogenesis, using human iPSC-organoids recapitulates APOE4-related phenotypes.

Mon Nov 02 00:00:00 UTC 2020

APOE4 exacerbates synapse loss and neurodegeneration in Alzheimer's disease patient iPSC-derived cerebral organoids.

Nature communications

14-9749 was used in Western Blotting to suggest APOE4-related degenerative pathways contributing to AD pathogenesis, using human iPSC-organoids recapitulates APOE4-related phenotypes.

Mon Nov 02 00:00:00 UTC 2020

Depletion of the AD Risk Gene SORL1 Selectively Impairs Neuronal Endosomal Traffic Independent of Amyloidogenic APP Processing.

Cell reports

Knupp A,Mishra S,Martinez R,Braggin JE,Szabo M,Kinoshita C,Hailey DW,Small SA,Jayadev S,Young JE

14-9749 was used in Immunocytochemistry-immunoflourescence to validate defects in neuronal endosomal trafficking following the depletion of the SORL1.

Tue Jun 02 00:00:00 UTC 2020

Acidifying Endolysosomes Prevented Low-Density Lipoprotein-Induced Amyloidogenesis.

Journal of Alzheimer's disease : JAD

Hui L,Soliman ML,Geiger NH,Miller NM,Afghah Z,Lakpa KL,Chen X,Geiger JD

14-9749-82 was used in Western Blot to study the mechanisms by which increased levels of circulating cholesterol increases the risk of Alzheimer's disease.

Mon Mar 02 00:00:00 UTC 2020

生物信息学

蛋白别名: ABPP; AG; Alzheimer disease amyloid A4 protein homolog; Alzheimer disease amyloid protein; amyloid A4; Amyloid b precursor protein; amyloid beta (A4) precursor protein; amyloid beta A4 protein; Amyloid precursor protein; Amyloid β precursor protein; Amyloid-beta (A4) precursor protein; Amyloid-beta A4 protein; Amyloid-beta precursor protein; Amyloidogenic glycoprotein; APP; APPI; appican; beta-amyloid peptide; beta-amyloid peptide(1-40); beta-amyloid peptide(1-42); beta-amyloid precursor protein; Cerebral vascular amyloid peptide; CVAP; Pan-Abeta; peptidase nexin-II; PN-II; PreA4; protease nexin II; Protease nexin-II; testicular tissue protein Li 2

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基因别名: A4; AAA; ABETA; ABPP; AD1; Adap; Ag; APP; APPI; betaApp; CTFgamma; CVAP; E030013M08Rik; PN-II; PN2

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UniProt ID: (Human) P05067, (Rat) P08592, (Mouse) P12023

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Entrez Gene ID: (Human) 351, (Rat) 54226, (Mouse) 11820

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功能