Invitrogen

ARFRP1 Polyclonal Antibody

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ARFRP1 Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF) — Figure 7. SYS1 is required for the association of ARFRP1, ARL1, GCC88, and GARP with the TGN. (A) Due to the lack of good antibodies to SYS1, KO of the SYS1 gene was demonstrated by Sanger sequencing (top) and genomic PCR (bottom). KO resulted in a 34- and 36-bp deletion. The 34-bp deletion is predicted to result in a frameshift causing premature termination at amino acid 28. The 36-bp deletion is not predicted to result in a frameshift, but to cause a deletion of more than half of the first transmembrane domain. (B) WT and SYS1-KO cells were immunostained for endogenous ARL1 or ARFRP1, counterstained with DAPI (blue), and imaged by confocal microscopy. (C) SYS1-KO cells were co-transfected with plasmids encoding VPS54-13Myc and either GFP alone or SYS1-GFP as indicated in the figure, immunostained for the Myc epitope, counterstained with DAPI (blue), and imaged by confocal microscopy. Insets are magnified views of the boxed areas. Inset scale bars: 5 mum. (D) SYS1-KO cells were transfected with plasmids encoding GFP or SYS1-GFP as indicated in the figure, immunostained for endogenous GCC88, counterstained with DAPI (blue), and imaged by confocal microscopy. Scale bars in B, C, and D: 10 mum. (E) Quantification of cells having VPS54-13Myc staining at the TGN as described in Fig. 2 C . Values are the mean +- SEM from three independent experiments. More than 100 cells per sample were counted in each experiment. (F) Quantification of cells having GCC88 staining at the TGN as des

ARFRP1 Antibody in Immunocytochemistry (ICC/IF) — Figure 6. ARFRP1 functions upstream of ARL1 in the recruitment of GCC88 to the TGN. (A) ARL1-KO cells were transfected with plasmids encoding GFP-tagged ARL1 (WT, Q71L, or T31N) or GFP-tagged ARFRP1 (WT, Q79L, or T31N), immunostained for endogenous GCC88, counterstained with DAPI (blue), and imaged by confocal microscopy. (B) The percentage of cells having GCC88 staining at the TGN from experiments such as that in panel A was quantified as described in Fig. 2 C . Values are the mean +- SEM from three independent experiments. More than 100 cells per sample were counted in each experiment. ***, P < 0.001, in comparison to untransfected ARL1 KO cells using Dunnett's test. (C) ARFRP1-KO cells were transfected and analyzed as described in A. (D) The percentage of cells having GCC88 staining at the TGN from experiments such as that in C was quantified as described in Fig. 2 C . Values are the mean +- SEM from three independent experiments. More than 100 cells per sample were counted in each experiment. ***, P < 0.001, in comparison to untransfected ARFRP1 KO cells using Dunnett's test. (E) WT, ARL1-KO, or ARFRP1-KO cells were immunostained for endogenous ARL1 or ARFRP1, counterstained with DAPI (blue), and imaged by confocal microscopy. Scale bars: 10 mum. Insets are magnified views of the boxed areas. Inset scale bars: 5 mum.

ARFRP1 Antibody in Western Blot (WB) — Figure 3. ARFRP1 functions upstream of ARL5 for the recruitment of GARP to the TGN. (A) GFP-tagged ARL5B (WT, Q70L, or T30N) or ARFRP1 (WT, Q79L, or T31N) were co-expressed with VPS54-13Myc in ARL5-KO cells. Cells were stained with DAPI (blue) and examined for GFP distribution by confocal microscopy. Scale bars: 10 mum. Insets are magnified views of the boxed areas. Inset scale bars: 5 mum. (B) The percentage of cells with VPS54-13Myc staining at the TGN was quantified as described in Fig. 2 C . Values are the mean +- SEM from three independent experiments. More than 100 cells per sample were counted in each experiment. ***, P < 0.001, in comparison to ARL5 KO cells only expressing VPS54-13Myc (control) using Dunnett's test. (C) GFP-tagged ARL5B (WT, Q70L, or T30N) or ARFRP1 (WT, Q79L, or T31N) were co-expressed with VPS54-13Myc in ARFRP1-KO cells. Cells were analyzed as in A. (D) Quantification of cells having VPS54-13Myc staining at the TGN as described in Fig. 2 C . Values are the mean +- SEM from three independent experiments. More than 100 cells per sample were counted in each experiment. ***, P < 0.001, in comparison to ARFRP1 KO cells only expressing VPS54-13Myc (control) using Dunnett's test. (E) Subcellular fractionation of WT, ARFRP1-KO, and ARFRP1-KO-rescue HeLa cells. Whole cells and cytosolic and membrane (Memb) fractions obtained as described in Materials and methods were analyzed by SDS-PAGE and immunoblotting for the proteins indicated on the right. The positi

产品信息

PA5-50606

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	查看1篇已发表文章 1篇已发表文章
免疫组化（石蜡） (IHC (P))	1:25-1:100	-
免疫细胞化学 (ICC/IF)	-	查看1篇已发表文章 1篇已发表文章

产品规格
种属反应	Human, Mouse, Rat
已发表种属	Human
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic peptide corresponding to residues near the C terminal of human ADP-ribosylation factor related protein 1 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	3.3 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.4, with 40% glycerol
内含物	0.05% sodium azide
保存条件	-20°C
RRID	AB_2636059

产品详细信息

The antibody detects endogenous levels of total ARFRP1 protein.

靶标信息

The protein encoded by this gene is a membrane-associated GTP-ase which localizes to the plasma membrane and is related to the ADP-ribosylation factor (ARF) and ARF-like (ARL) proteins. This gene plays a role in membrane trafficking between the trans-Golgi network and endosomes. Alternatively spliced transcript variants encoding different isoforms have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network.

The Journal of cell biology

Ishida M,Bonifacino JS

Published figure using ARFRP1 polyclonal antibody (Product # PA5-50606) in Immunocytochemistry

Mon Nov 04 00:00:00 EST 2019

ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network.

The Journal of cell biology

Ishida M,Bonifacino JS

Published figure using ARFRP1 polyclonal antibody (Product # PA5-50606) in Immunocytochemistry

Mon Nov 04 00:00:00 EST 2019

ARFRP1 functions upstream of ARL1 and ARL5 to coordinate recruitment of distinct tethering factors to the trans-Golgi network.

The Journal of cell biology

Ishida M,Bonifacino JS

Published figure using ARFRP1 polyclonal antibody (Product # PA5-50606) in Immunocytochemistry

Mon Nov 04 00:00:00 EST 2019

生物信息学

蛋白别名: ADP-ribosylation factor related protein 1; ADP-ribosylation factor-related protein 1; ARF-related protein 1; ARP; helicase-like protein NHL; SCG10 like-protein

基因别名: 1500006I01Rik; AI480700; ARFRP1; ARL18; ARP; ARP1

UniProt ID: (Human) Q13795, (Mouse) Q8BXL7, (Rat) Q63055

Entrez Gene ID: (Human) 10139, (Mouse) 76688, (Rat) 117051

功能

small GTPase mediated signal transduction gastrulation protein localization to Golgi apparatus retrograde transport, endosome to Golgi Golgi to plasma membrane protein transport signal transduction

参与通路

GTPase activity protein binding GTP binding nucleotide binding

ARFRP1 Polyclonal Antibody

产品信息

PA5-50606

免疫印迹 (WB)

免疫组化（石蜡） (IHC (P))

免疫细胞化学 (ICC/IF)

种属反应

已发表种属

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

生物信息学

性能保障

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