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Invitrogen
ATG13 Polyclonal Antibody
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ATG13 Antibody (PA5-77806) in ICC/IF
Immunofluorescent analysis of ATG13 in mouse myoblast cell line (C2C12). Sample was fixed with 4% formaldehyde (15 min at RT), incubated with ATG13 polyclonal antibody (Product # PA5-77806) using a dilution of 1:100 (1 hour at RT), and followed by Goat Anti-Mouse 488 and DAPI secondary antibody at a dilution of 1:100 (60 min at RT) and 1:5000 (5 min at RT). Images are shown as follows: (A) DAPI (blue) nuclear stain B) Phalloidin Texas Red F-Actin stain C) ATG13 Antibody D) Merged image. Magnification: 60x.
产品信息
PA5-77806
产品规格
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Synthetic peptide from the mid-protein range of human ATG13 (C-DTETVSNSSEGR)
偶联物
形式
Liquid
浓度
1 mg/mL
保存条件
-20°C
运输条件
Wet ice
RRID
产品详细信息
A 1:1,000 dilution of PA5-77806 was sufficient for detection of ATG13 on HEK293 lysates using Goat anti-rabbit IgG:HRP as the secondary antibody.|Detects approximately 56-63kDa.
靶标信息
Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein ATG1. ATG13 forms a complex with ULK1 and ULK2, the mammalian homologs of ATG1, and with FIP200. This complex is a target of TOR phosphorylation under normal conditions; inhibition of TOR by rapamycin or leucine deprivation leads to dephosphorylation of ATG13, ULK1 and ULK2, which then leads to autophagy. Knockdown of ATG13 inhibits autophagosome formation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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