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ATG4B Antibody (710915) in ICC/IF
Immunofluorescent analysis of ATG4B was done on 70% confluent log phase Rapamycin treated HeLa cells (treated with 100nM of Rapamycin A for 4 hours). The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with ATG14 Recombinant Rabbit Polyclonal Antibody (Product # 710915) at 2 µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat anti-Rabbit IgG Secon... View More
产品信息
710915
产品规格
种属反应
Human,
Mouse
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
1HCLC
抗原
full-length protein corresponding to Human ATG4b
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2532779
产品详细信息
This antibody is predicted to react with monkey, mouse, rat, guinea pig and bovine based on sequence homology.
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
Cysteine protease ATG4b is an enzyme which is coded by ATG4b gene. Autophagy is a critical cellular homeostatic process that controls the turnover of damaged organelles and protein. ATG4b is a deubiquitin-like protease that plays dual roles in the core machinery of autophagy. ATG4b, cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. ATG4b is mainly expressed in the skeletal muscle, brain, heart, liver and pancreas.
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生物信息学
蛋白别名: APG4 (ATG4) autophagy-related homolog B; APG4 autophagy 4 homolog B; ATG4 autophagy related 4 homolog B; AUT-like 1 cysteine; AUT-like 1 cysteine endopeptidase; AUT-like 1, cysteine endopeptidase; autophagin 1; Autophagin-1; autophagy related 4B, cysteine peptidase; autophagy-related 4B; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; Cysteine protease ATG4B; cysteine protease involved in autophagy APG4-B; hAPG4B; HsAPG4B; MmAPG4B
基因别名: 2510009N07Rik; APG4B; ATG4B; Atg4bl; AUTL1; AW048066; KIAA0943
UniProt ID: (Human) Q9Y4P1, (Mouse) Q8BGE6
Entrez Gene ID: (Human) 23192, (Mouse) 66615
autophagosome assembly
mitophagy
C-terminal protein lipidation
proteolysis
protein targeting to membrane
autophagy
macroautophagy
protein processing
nucleophagy
positive regulation of protein catabolic process
protein delipidation
transport
cellular response to nitrogen starvation
positive regulation of autophagy
protein transport
