Invitrogen

ATG9B Polyclonal Antibody

ATG9B Antibody in Immunocytochemistry (ICC/IF)

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ATG9B Antibody (PA5-20998) in ICC/IF

Immunocytochemistry of ATG9B in HeLa cells with ATG9B Polyclonal Antibody (Product # PA5-20998) at 10 µg/mL.

ATG9B Antibody in Western Blot, Immunocytochemistry (WB, ICC/IF)

Product Image Gallery

ATG9B Antibody — Figure 6 Fisetin induces autophagy and lysosomal genes. ( a,b ) Mouse cortical cells (T4) and rat primary cortical neurons were treated with 5 and 10 muM fisetin for 24 h, respectively. Quantitative real time PCR (qRT-PCR) was performed using primer sets of genes of interest following the procedure described in Methods. Bar graphs represent the relative mRNA level of genes in T4 cells ( a ) and neurons ( b ) compared to those in cells not treated with fisetin. ( c,d ) T4 cells and neurons were treated with fisetin (FST) for 24 h and 36 h, respectively. The levels of TFEB, Nrf2, ATG9b, NDP52, p62/SQSTM1 and HO-1 in T4 cells ( c ) and neurons ( d ) were analyzed by immunoblotting using each corresponding antibody, respectively. Data shown are mean +- SE of three independent experiments and were analyzed using Student's t test. (* p < 0.05; ** p < 0.01; *** p < 0.001). {TM}

产品信息

PA5-20998

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1-2 µg/mL

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免疫细胞化学 (ICC/IF)

10-20 µg/mL

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A 17 amino acid synthetic peptide near the carboxy terminus of human ATG9B. The immunogen is located within amino acids 850-900 of ATG9B.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

0.02% sodium azide

保存条件

Maintain refrigerated at 2-8°C for up to 3 months. For long term storage store at -20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_11153916

产品详细信息

A suggested positive control is Hela cell lysate.

PA5-20998 can be used with blocking peptide PEP-1112.

靶标信息

Autophagy, the process of bulk degradation of cellular proteins through an autophagosomic-lysosomal pathway is important for normal growth control and may be defective in tumor cells. It is involved in the preservation of cellular nutrients under starvation conditions as well as the normal turnover of cytosolic components. This process is negatively regulated by TOR (Target of rapamycin) through phosphorylation of autophagy protein APG1. ATG9B plays a role in autophagy and it's highly expressed in placenta and pituitary gland.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Human papillomavirus 16E6/E7 activates autophagy via Atg9B and LAMP1 in cervical cancer cells.

Cancer medicine

Tingting C,Shizhou Y,Songfa Z,Junfen X,Weiguo L,Xiaodong C,Xing X

PA5-20998 was used in Western Blotting to find that HPV 16E6/E7 (16E6/E7) downregulation inhibits autophagy, and consequently suppresses cell proliferation and promotes early apoptosis.

Thu Aug 01 00:00:00 UTC 2019

Human papillomavirus 16E6/E7 activates autophagy via Atg9B and LAMP1 in cervical cancer cells.

Cancer medicine

Tingting C,Shizhou Y,Songfa Z,Junfen X,Weiguo L,Xiaodong C,Xing X

PA5-20998 was used in Western Blotting to find that HPV 16E6/E7 (16E6/E7) downregulation inhibits autophagy, and consequently suppresses cell proliferation and promotes early apoptosis.

Thu Aug 01 00:00:00 UTC 2019

Human papillomavirus 16E6/E7 activates autophagy via Atg9B and LAMP1 in cervical cancer cells.

Cancer medicine

Tingting C,Shizhou Y,Songfa Z,Junfen X,Weiguo L,Xiaodong C,Xing X

PA5-20998 was used in Western Blotting to find that HPV 16E6/E7 (16E6/E7) downregulation inhibits autophagy, and consequently suppresses cell proliferation and promotes early apoptosis.

Thu Aug 01 00:00:00 UTC 2019

Fisetin stimulates autophagic degradation of phosphorylated tau via the activation of TFEB and Nrf2 transcription factors.

Scientific reports

Kim S,Choi KJ,Cho SJ,Yun SM,Jeon JP,Koh YH,Song J,Johnson GV,Jo C

PA5-20998 was used in western blot to decipher activation of TFEB and Nrf2 transcription factors and stimulation by Fisetin for autophagic degradation of phosphorylated tau

Tue Apr 26 00:00:00 UTC 2016

生物信息学

蛋白别名: APG9-like 2; ATG9; ATG9 autophagy related 9 homolog B; autophagy 9-like 2 protein; Autophagy-related protein 9B; endothelial nitric oxide synthase antisense; nitric oxide synthase 3 antisense; Nitric oxide synthase 3-overlapping antisense gene protein; Protein sONE

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基因别名: Apg912; APG9L2; Apgdc2; ATG9B; eONE; Gm574; NOS3AS; RGD1560887; SONE

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UniProt ID: (Human) Q674R7, (Mouse) Q6EBV9

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Entrez Gene ID: (Human) 285973, (Rat) 499973, (Mouse) 213948

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功能

molecular_function

参与通路

autophagosome assembly mitophagy autophagy protein localization to pre-autophagosomal structure piecemeal microautophagy of nucleus late nucleophagy