Invitrogen

ATP Synthase beta Monoclonal Antibody (3D5AB1)

Name: ATP Synthase beta Monoclonal Antibody (3D5AB1)
Brand: Invitrogen
SKU: A-21351

高级验证

48个已发表图片

96 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

ATP Synthase beta Antibody in Immunocytochemistry (ICC/IF)

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ATP Synthase beta Antibody (A-21351) in ICC/IF

Immunofluorescent analysis of ATP Synthase Subunit beta was performed using 70% confluent log phase Hep G2 cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with ATP Synthase Subunit beta (3D5AB1) Mouse Monoclonal Antibody (Product # A-21351) at 2 µg/mL in 0... View More

ATP Synthase beta Antibody in Western Blot (WB)

ATP Synthase beta Antibody in Immunohistochemistry (IHC)

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Immunogen image

Immunogen Image ATP Synthase beta Monoclonal Antibody (3D5AB1)

产品信息

A-21351

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.5 µg/mL

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

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免疫细胞化学 (ICC/IF)

1-2 µg/mL

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流式细胞分析 (Flow)

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酶联免疫吸附实验 (ELISA)

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免疫沉淀 (IP)

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亲和纯化 (AP)

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其他PubMed (Misc)

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产品规格

种属反应

Bovine, C. elegans, Human, Mouse, Non-human primate, Rat

已发表种属

C. elegans, Fruit fly, Human, Mouse, Pig, Rat

宿主/亚型

Mouse / IgG1, kappa

分类

Monoclonal

类型

Antibody

克隆号

3D5AB1

抗原

Human Heart Mitochondria.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

purified

保存液

HEPES buffered saline

内含物

0.02% sodium azide

保存条件

4°C

运输条件

Ambient (domestic); Wet ice (international)

AB_221512

产品详细信息

The antibody was produced in vitro using hybridomas grown in serum-free medium, and then purified by biochemical fractionation. Near homogeneity was judged by SDS-PAGE.

The epitope recognized by the anti-ATP synthase beta 3D5AB1 is in the region containing the active site of the beta-subunit and is centered approximately at amino acid residue 83. The complete amino acid sequence of the epitope is not known. See Chi SL, Wahl ML, Kenan DJ et al. (2007) Angiostatin-like activity of a monoclonal antibody to the catalytic subunit of F1F0 ATP synthase. Cancer Res 67:4716-4724.

靶标信息

ATP synthase is extremely conserved through evolution and can be found in plants, fungi, bacteria, and animals. The ATP synthase enzyme is a transmembrane protein responsible for driving the reversible reaction from ADP+ phosphate to ATP. This reaction is accomplished by a flux of protons across the membrane as a result of electron transfer. The ATP synthase protein has two main sections; the F1 ATP-ase (soluble) and the F0 ATP-ase (membrane embedded). The F1 section consists of the alpha, beta, gamma, delta, and epsilon subunits. While the F0 consists of a, b, and c subunits.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: ATP synthase F1 subunit beta; ATP synthase subunit beta, mitochondrial; ATP synthase, H+ transporting mitochondrial F1 complex, alpha subunit; ATP synthase, H+ transporting mitochondrial F1 complex, beta subunit; ATP synthase, H+ transporting, mitochondrial F1 complex, beta polypeptide; epididymis secretory protein Li 271; F-1-ATPase beta-subunit precursor; F1-ATPase beta-subunit; MGC5231; mitochondrial ATP synthase beta subunit; mitochondrial ATP synthase, H+ transporting F1 complex beta subunit; mitochondrial ATP synthetase, beta subunit

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基因别名: ATP5B; ATP5F1B; ATPMB; ATPSB; HEL-S-271