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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • ATP1A1 Antibodies

          Invitrogen

          ATP1A1 Monoclonal Antibody (M8-P1-A3)

          高级验证
          8个已发表图片
          47 篇参考文献
          查看其他27个ATP1A1抗体

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          说明书
          实验方案
          问题与答案
          技术支持
          说明书
          实验方案
          问题与答案
          技术支持

          Cite ATP1A1 Monoclonal Antibody (M8-P1-A3)

          • 抗体检测数据 (11)
          • 已发表图片 (8)
          • 高级验证 (2)
          ATP1A1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.
          ATP1A1 Antibody in Immunocytochemistry (ICC/IF)
          Group 53 Created with Sketch.

          图: 1 / 21

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          ATP1A1 Antibody (MA3-929) in ICC/IF

          Immunofluorescent analysis of sodiµm/potassium ATPase alpha-1 (green) in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 15 minutes at room temperature and blocked with 0.3% BSA for 15 minutes at room temperature. Cells were probed with a sodiµm/potassium ATPase alpha-1 monoclonal antibody (Product # MA3-929) at a dilution of 1:100 for at least 1 hour at room temperature, washed with PBS, and incubated with a DyLight 488-conjugated goat anti-mouse IgG secondary antibody (Product # 35502) at a dilution ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
          已发表图片由___提供 benchsci-logo
          PMID: {{$ctrl.currentElement.benchSciPubmedId}}
          查看产品

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          ATP1A1 Antibody in Immunocytochemistry (ICC/IF)
          ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATP1A1 Antibody in Immunohistochemistry (Paraffin) (IHC (P))
          ATP1A1 Antibody in Western Blot (WB)
          ATP1A1 Antibody in Western Blot (WB)
          ATP1A1 Antibody in Western Blot (WB)
          ATP1A1 Antibody in Immunocytochemistry (ICC/IF)
          ATP1A1 Antibody in Western Blot (WB)
          ATP1A1 Antibody in Western Blot (WB)
          ATP1A1 Antibody in Western Blot (WB)
          ATP1A1 Antibody in Western Blot (WB)
          ATP1A1 Antibody in Western Blot (WB)
          ATP1A1 Antibody in Western Blot (WB)
          ATP1A1 Antibody in Western Blot (WB)
          ATP1A1 Antibody
          ATP1A1 Antibody

          产品信息

          MA3-929

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          1:200-1:2,000
          查看35篇已发表文章 35篇已发表文章

          免疫组化 (IHC)

          -
          查看4篇已发表文章 4篇已发表文章

          免疫组化(石蜡) (IHC (P))

          1:100-1:2,000
          -

          免疫组化(冰冻) (IHC (F))

          1:100
          -

          免疫细胞化学 (ICC/IF)

          1/50 - 1/200
          查看7篇已发表文章 7篇已发表文章

          流式细胞分析 (Flow)

          1/20 - 1/100
          -

          中和实验 (Neu)

          -
          查看1篇已发表文章 1篇已发表文章
          产品规格

          种属反应

          Dog, Human, Mouse, Sheep, Pig, Rat

          已发表种属

          Bovine, Dog, Human, Invertebrate, Mouse, Non-human primate, Pig, Rat, Sheep, Yeast

          宿主/亚型

          Mouse / IgG1

          分类

          Monoclonal

          类型

          Antibody

          克隆号

          M8-P1-A3

          抗原

          Lamb kidney alpha 1 sodium/potassium ATPase.
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Liquid

          浓度

          1 mg/mL

          纯化类型

          Protein A

          保存液

          PBS with 1mg/mL BSA

          内含物

          0.05% sodium azide

          保存条件

          -20°C, Avoid Freeze/Thaw Cycles

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2061127

          产品详细信息

          MA3-929 detects sodium/potassium ATPase alpha 1 in sheep, canine, rat, mouse, human and porcine tissues. This antibody does not detect the alpha 1 isoform in Xenopus and chicken. MA3-929 does not detect the alpha 2 or alpha 3 isoform in rat.

          MA3-929 has been successfully used in Western blot, immunofluorescence, immunocytochemistry, flow cytometry, and immunohistochemistry procedures. By Western blot, this antibody detects an ~110 kDa protein representing alpha 1 sodium/potassium ATPase from canine kidney extract. Immunohistochemical staining of sodium/potassium ATPase alpha 1 with MA3-929 yields a pattern consistent with plasma membrane localization.

          The MA3-929 antigen is sheep kidney alpha 1 sodium/potassium ATPase. This antibody recognizes an epitope between amino acid residues 496-506 of lamb kidney sodium/potassium ATPase.

          靶标信息

          The protein encoded by this gene belongs to the family of P-type cation transport ATPases, and to the subfamily of Na+/K+ -ATPases. Na+/K+ -ATPase is an integral membrane protein responsible for establishing and maintaining the electrochemical gradients of Na and K ions across the plasma membrane. These gradients are essential for osmoregulation, for sodium-coupled transport of a variety of organic and inorganic molecules, and for electrical excitability of nerve and muscle. This enzyme is composed of two subunits, a large catalytic subunit (alpha) and a smaller glycoprotein subunit (beta). The catalytic subunit of Na+/K+ -ATPase is encoded by multiple genes. This gene encodes an alpha 1 subunit. Multiple transcript variants encoding different isoforms have been found for this gene.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          生物信息学

          蛋白别名: (Na+, K+)-ATPase alpha-subunit; ATP1B; ATPase, Na+/K+ transporting, alpha 1 polypeptide; ATPase, Na+K+ transporting, alpha 1; MGC1798; Na(+)/K(+) ATPase alpha-1 subunit; Na+ ,K+ -ATPase alpha subunit; Na+ /K+ ATPase alpha 1 subunit; Na+, K+-ATPase beta-subunit precursor; Na+/K+ ATPase 1; Na+/K+ ATPase Alpha; Na+/K+ transporting alpha 1 polypeptide; Na, K-ATPase, alpha-A catalytic polypeptide; Na,K-ATPase alpha-1 subunit; Na,K-ATPase catalytic subunit alpha-A protein; RP4-655J12.1; Sodium Potassium ATPase; sodium pump 1; Sodium pump subunit alpha-1; sodium-potassium ATPase catalytic subunit alpha-1; sodium-potassium-ATPase, alpha 1 polypeptide; sodium/potassium-transporting ATPase alpha-1 chain; Sodium/potassium-transporting ATPase subunit alpha-1; unnamed protein product

          查看更多 收起

          基因别名: ATP1A1; Atpa-1; BC010319; Nkaa1b

          查看更多 收起

          UniProt ID: (Sheep) P04074, (Human) P05023, (Pig) P05024, (Rat) P06685, (Mouse) Q8VDN2

          查看更多 收起

          Entrez Gene ID: (Dog) 403992, (Sheep) 443381, (Human) 476, (Pig) 397481, (Rat) 24211, (Mouse) 11928

          查看更多 收起

          功能
          sodium:potassium-exchanging ATPase activity ATP binding metal ion binding protein binding phosphatase activity protein kinase binding protein domain specific binding ankyrin binding potassium ion binding sodium ion binding ADP binding phosphatidylinositol 3-kinase binding chaperone binding steroid hormone binding ATPase binding nucleotide binding hydrolase activity primary active transporter
          参与通路
          regulation of sodium ion transport sodium ion transport establishment or maintenance of transmembrane electrochemical gradient potassium ion transmembrane transport regulation of the force of heart contraction cellular sodium ion homeostasis regulation of blood pressure potassium ion import ATP hydrolysis coupled proton transport dephosphorylation cellular potassium ion homeostasis negative regulation of glucocorticoid biosynthetic process ion transmembrane transport sodium ion export from cell response to drug negative regulation of heart contraction positive regulation of heart contraction positive regulation of striated muscle contraction relaxation of cardiac muscle cardiac muscle contraction membrane hyperpolarization cellular response to mechanical stimulus cellular response to steroid hormone stimulus regulation of cardiac muscle cell contraction membrane repolarization membrane repolarization during cardiac muscle cell action potential cell communication by electrical coupling involved in cardiac conduction response to glycoside regulation of cardiac conduction potassium ion import across plasma membrane transmembrane transport potassium ion transport energy coupled proton transmembrane transport, against electrochemical gradient sodium ion transmembrane transport transport ion transport
          It has to be done as per old AB suggested Products section.
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          性能保障

          只要您发现Invitrogen抗体在实验中的表现与网站或说明书的所述内容不符,您即可享受免费退换货服务。*

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