Invitrogen

ATP5G1/2/3 Polyclonal Antibody

ATP5G1/2/3 Antibody in Immunocytochemistry (ICC/IF)

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ATP5G1/2/3 Antibody (PA5-114397) in ICC/IF

Immunocytochemistry/Immunofluorescence analysis of ATP5G1/ATP5G2/ATP5G3 in A549 cells using ATP5G1/2/3 Polyclonal Antibody (Product # PA5-114397). Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum and incubated with the primary antibody at 5 µg/mL. DyLight 488 conjugated goat anti-rabbit IgG was used as secondary antibod... View More

ATP5G1/2/3 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

ATP5G1/2/3 Antibody in Western Blot (WB)

ATP5G1/2/3 Antibody in Flow Cytometry (Flow)

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产品信息

PA5-114397

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.5 µg/mL

免疫组化（石蜡） (IHC (P))

0.5-1 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

流式细胞分析 (Flow)

1-3 µg/1x10^6 cells

酶联免疫吸附实验 (ELISA)

0.1-0.5 µg/mL

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived human ATP5F1/ATP5MC1 recombinant protein (Position: D62-L113).

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

500 µg/mL

纯化类型

Affinity chromatography

保存液

PBS with 4mg trehalose

内含物

0.05mg sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2884853

产品详细信息

Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

Positive Control - WB: human HEPG2 whole cell, human A549 whole cell, human PC-3 whole cell, human HL-60 whole cell, human HEK293 whole cell, human U937 whole cell, human CACO-2 whole cell, rat intestine tissue, human Ovarian cancer tissue, human tonsil tissue, human glioma tissue, human lung cancer tissue, human placenta tissue, mouse intestine tissue. IHC: human intestinal tissue. Flow: HL-60 cell, PC-3 cell.|Store at -20°C for one year from date of receipt. After reconstitution, at 4°C for one month. It can also be aliquotted and stored frozen at -20°C for six months. Avoid repeated freeze-thaw cycles.

靶标信息

The ATP5MC1 gene is one of three human paralogs that encode membrane subunit c of the mitochondrial ATP synthase. It is mapped to 17q21.32. This gene encodes a subunit of mitochondrial ATP synthase. Mitochondrial ATP synthase catalyzes ATP synthesis, utilizing an electrochemical gradient of protons across the inner membrane during oxidative phosphorylation. ATP synthase is composed of two linked multi-subunit complexes: the soluble catalytic core, F1, and the membrane-spanning component, Fo, comprising the proton channel. The catalytic portion of mitochondrial ATP synthase consists of 5 different subunits (alpha, beta, gamma, delta, and epsilon) assembled with a stoichiometry of 3 alpha, 3 beta, and a single representative of the other 3. The proton channel seems to have nine subunits (a, b, c, d, e, f, g, F6 and 8). This gene is one of three genes that encode subunit c of the proton channel. Each of the three genes have distinct mitochondrial import sequences but encode the identical mature protein. Alternatively spliced transcript variants encoding the same protein have been identified.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: ATP synthase c subunit; ATP synthase F(0) complex subunit C1, mitochondrial; ATP synthase F(0) complex subunit C2, mitochondrial; ATP synthase F(0) complex subunit C3, mitochondrial; ATP synthase lipid-binding protein; ATP synthase lipid-binding protein, mitochondrial; ATP synthase membrane subunit c locus 1; ATP synthase membrane subunit c locus 2; ATP synthase membrane subunit c locus 3; ATP synthase proteolipid P1; ATP synthase proteolipid P2; ATP synthase proteolipid P3; ATP synthase proton-transporting mitochondrial F(0) complex subunit C1; ATP synthase proton-transporting mitochondrial F(0) complex subunit C2; ATP synthase proton-transporting mitochondrial F(0) complex subunit C3; ATP synthase subunit 9; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 1; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 2; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit c (subunit 9), isoform 3; ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C1 (subunit 9); ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C2 (subunit 9); ATP synthase, H+ transporting, mitochondrial F0 complex, subunit C3 (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C1 (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C2 (subunit 9); ATP synthase, H+ transporting, mitochondrial Fo complex, subunit C3 (subunit 9); ATP synthase, mitochondrial, C subunit-3; ATPase protein 9; ATPase subunit 9; ATPase subunit C; mitochondrial ATP synthase, subunit 9, isoform 1; mitochondrial ATP synthase, subunit C (subunit 9), isoform 2; mitochondrial ATP synthase, subunit C, isoform 1

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基因别名: 1810041M08Rik; 6030447M23; ATP5A; ATP5G; ATP5G1; ATP5G2; ATP5G3; ATP5MC1; ATP5MC2; ATP5MC3; P3; PSEC0033