Invitrogen

ATR Recombinant Superclonal Antibody (23HCLC)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

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说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (1)
高级验证 (1)

ATR Antibody in Immunocytochemistry (ICC/IF)

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ATR Antibody (710746) in ICC/IF

For immunofluorescence analysis, Hydroxy Urea (3 mM, 20h) treated HeLa cells were fixed and permeabilized for detection of ATR using ATR Recombinant Rabbit Polyclonal Antibody (Product # 710746, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of ATR protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Prod... View More

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ATR Antibody — Detection of altered subcellular localization of the target protein by cell treatment demonstrates antibody specificity. Immunofluorescence analysis of ATR using Anti-ATR Recombinant Rabbit Polyclonal Antibody (Product # 710746), shows translocation from cytoplasm to nucleus with an increase in expression of ATR upon hydroxy urea treatment. Hydroxy urea induces DNA damage, which results in the activation of ATR, its localization to the sites of DNA damage, and an elevation in its kinase activity. {TM}

产品信息

710746

应用

建议稀释比

已发表文章

免疫细胞化学 (ICC/IF)

2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Superclonal

类型

Antibody

克隆号

23HCLC

抗原

Peptides corresponding to Human ATR (aa 7-26, 442-459)

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2607919

产品详细信息

This antibody is predicted to react with Monkey, Cat and Bovine.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

靶标信息

The protein encoded by this gene is a serine/threonine kinase and DNA damage sensor, activating cell cycle checkpoint signaling upon DNA stress. The encoded protein can phosphorylate and activate several proteins involved in the inhibition of DNA replication and mitosis, and can promote DNA repair, recombination, and apoptosis. This protein is also important for fragile site stability and centrosome duplication. Defects in this gene are a cause of Seckel syndrome 1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: 2310008J16Rik; 2810405N18Rik; Ataxia telangiectasia and Rad3-related protein; EC 2.7.11.1; FRAP-related protein 1; FRAP-related protein-1; kinase ATR; MEC1, mitosis entry checkpoint 1, homolog; protein kinase ATR; Serine/threonine-protein kinase ATR; TEM8

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基因别名: ATR; FCTCS; FRP1; MEC1; SCKL; SCKL1

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UniProt ID: (Human) Q13535

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Entrez Gene ID: (Human) 545

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功能

DNA binding protein kinase activity protein serine/threonine kinase activity protein binding ATP binding MutLalpha complex binding MutSalpha complex binding non-receptor serine/threonine protein kinase protein modifying enzyme

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