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Rockland
Acetyl-HSP90 (Lys294) Polyclonal Antibody
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Acetyl-HSP90 (Lys294) Antibody (600-401-981) in IHC (P)
Rocklands affinity purified anti-Hsp90 acetyl K294 antibody was used at 20 µg/ml to detect signal in a variety of tissues including multi-human, multi-brain and multi-cancer slides. This image shows moderate nuclear and granular cytoplasmic positive staining in human prostate carcinoma at 40X. Tissue was formalin-fixed and paraffin embedded. The image shows localization of the antibody as the precipitated r... View More
Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.
产品信息
600-401-981
应用
建议稀释比
已发表文章
产品规格
种属反应
Human,
Mouse,
Rat
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
This affinity purified antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids surrounding K294 of human Hsp90.
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Liquid
浓度
0.96 mg/mL
规格
100 µg
纯化类型
Affinity chromatography
保存液
0.02M potassium phosphate, pH 7.2, with 0.15M NaCl
内含物
0.01% sodium azide
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
While ELISA data show strong reactivity with the acetylated form of the immunizing peptide and minimal reactivity with the non-acetylated form, to date western blotting data are not definitive for acetyl K294 specificity as blots show equivalent staining of lysates from cells either treated or untreated with Trichostatin A (an HDAC inhibitor). A BLAST analysis was used to suggest cross-reactivity with Hsp90 from human, mouse, rat, monkey, chicken and Drosophila based on 100% homology with the immunizing sequence. Reactivity of this antibody with Hsp90 from other species is unknown.
靶标信息
Heat shock proteins (HSP) are proteins that are expressed in response to various biological stress, including heat. HSP90 is a 90 kDa protein that is induced under stress conditions, but is also one of the most abundant cellular proteins found under non-stress conditions. HSP90 has been found to be associated with a number of other intracellular proteins, including steroid receptors, actin, tubulin, and some kinases. It has been suggested that HSP90 is necessary to maintain structural integrity of at least some steroid receptors to allow for ligand binding during receptor activation.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: Heat shock 86 kDa; Heat shock protein; heat shock protein 1, alpha; heat shock protein 86; heat shock protein 90kDa alpha (cytosolic), class A member 1; Heat shock protein family C member 1; Heat shock protein HSP 90-alpha; heat shock protein, 1; heat shock protein, 86 kDa 1; heat shock protein, 89 kDa; Heat shock protein90; HSP; HSP 86; HSP 90 alpha; HSP86; LAP-2; Lipopolysaccharide-associated protein 2; Renal carcinoma antigen NY-REN-38; TSTA; Tumor-specific transplantation 86 kDa antigen
基因别名: 86kDa; 89kDa; AL024080; AL024147; hsp4; Hsp86; Hsp86-1; Hsp89; Hsp90; HSP90A; HSP90AA1; HSPC1; HSPCA
UniProt ID: (Mouse) P07901, (Rat) P82995
Entrez Gene ID: (Mouse) 15519, (Rat) 299331
nucleotide binding
UTP binding
CTP binding
mRNA binding
protein binding
ATP binding
GTP binding
ATPase activity
sulfonylurea receptor binding
protein kinase binding
protein phosphatase binding
MHC class II protein complex binding
nitric-oxide synthase regulator activity
TPR domain binding
dATP binding
identical protein binding
protein homodimerization activity
ion channel binding
poly(A) RNA binding
GTPase binding
Rho GDP-dissociation inhibitor binding
unfolded protein binding
Hsp90 family chaperone
neuron migration
protein folding
nitric oxide biosynthetic process
response to stress
response to unfolded protein
positive regulation of lamellipodium assembly
positive regulation of protein import into nucleus, translocation
protein refolding
protein import into mitochondrial outer membrane
positive regulation of nitric oxide biosynthetic process
positive regulation of cytotoxic T cell differentiation
positive regulation of cell size
regulation of catalytic activity
chaperone-mediated protein complex assembly
skeletal muscle contraction
response to heat
response to salt stress
cardiac muscle cell apoptotic process
response to estrogen
positive regulation of cardiac muscle contraction
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Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.
