Invitrogen

Adenylate Kinase 4 Polyclonal Antibody

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Adenylate Kinase 4 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of Adenylate Kinase 4 was performed in HeLa cells using Adenylate Kinase 4 Polyclonal Antibody (Product # PA5-96048).

Adenylate Kinase 4 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of Adenylate Kinase 4 in paraffin-embedded rat kidney using Adenylate Kinase 4 Polyclonal Antibody (Product # PA5-96048) at a dilution of 1:200.

Adenylate Kinase 4 Antibody in Western Blot (WB) — Western Blot analysis of Adenylate Kinase 4 in extracts of various cell lines using Adenylate Kinase 4 Polyclonal Antibody (Product # PA5-96048) at a dilution of 1:1000. A HRP Goat Anti-Rabbit IgG (H+L) secondary antibody was used at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST.

Adenylate Kinase 4 Antibody in Immunoprecipitation (IP) — Immunoprecipitation analysis of Adenylate Kinase 4 was performed in 200 µg extracts of HepG2 cells using Adenylate Kinase 4 Polyclonal Antibody (Product # PA5-96048). Western blot was performed from the immunoprecipitate using Adenylate Kinase 4 Polyclonal Antibody.

产品信息

PA5-96048

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	-
免疫组化（石蜡） (IHC (P))	1:50 - 1:200	-
免疫细胞化学 (ICC/IF)	1:50 - 1:200	-
免疫沉淀 (IP)	1:50 - 1:100	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant fusion protein containing a sequence corresponding to amino acids 1-223 of human AK4.
偶联物	Unconjugated
形式	Liquid
浓度	1.21 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.3, with 50% glycerol
内含物	0.02% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2807850

产品详细信息

Immunogen sequence: MASKLLRAVI LGPPGSGKGT VCQRIAQNFG LQHLSSGHFL RENIKASTEV GEMAKQYIEK SLLVPDHVIT RLMMSELENR RGQHWLLDGF PRTLGQAEAL DKICEVDLVI SLNIPFETLK DRLSRRWIHP PSGRVYNLDF NPPHVHGIDD VTGEPLVQQE DDKPEAVAAR LRQYKDVAKP VIELYKSRGV LHQFSGTETN KIWPYVYTLF SNKITPIQSK EAY; Positive Samples: HepG2, Raji, SW480, Mouse liver, Mouse kidney; Cellular Location: Mitochondrion matrix

靶标信息

This gene encodes a member of the adenylate kinase family of enzymes. The encoded protein is localized to the mitochondrial matrix. Adenylate kinases regulate the adenine and guanine nucleotide compositions within a cell by catalyzing the reversible transfer of phosphate group among these nucleotides. Five isozymes of adenylate kinase have been identified in vertebrates. Expression of these isozymes is tissue-specific and developmentally regulated. A pseudogene for this gene has been located on chromosome 17. Three transcript variants encoding the same protein have been identified for this gene. Sequence alignment suggests that the gene defined by NM_013410, NM_203464, and NM_001005353 is located on chromosome 1.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: adenylate kinase 3 alpha-like 1; Adenylate kinase 3-like; adenylate kinase 3-like 1; adenylate kinase 3-like 2; adenylate kinase 3-like {ECO:0000255|HAMAP-Rule:MF_03170}; Adenylate kinase 4, mitochondrial; adenylate kinase 4, mitochondrial {ECO:0000255|HAMAP-Rule:MF_03170}; Adenylate kinase isoenzyme 4; adenylate kinase isoenzyme 4, mitochondrial; AK 4; AK 4 {ECO:0000255|HAMAP-Rule:MF_03170}; AK4; ATP-AMP transphosphorylase; GTP:AMP phosphotransferase AK4; GTP:AMP phosphotransferase AK4 {ECO:0000255|HAMAP-Rule:MF_03170}; GTP:AMP phosphotransferase AK4, mitochondrial; mitochondrial adenylate kinase-3; nucleoside-triphosphate-adenylate kinase; RP4-686B20.1

基因别名: AK 4; Ak-3; Ak-4; AK3; Ak3b; AK3L1; AK3L2; AK4; D4Ertd274e

UniProt ID: (Human) P27144, (Rat) Q9WUS0, (Mouse) Q9WUR9

Entrez Gene ID: (Human) 205, (Rat) 29223, (Mouse) 11639

分子生物学功能: nucleotide kinase