Invitrogen

Adiponectin Receptor 1 Polyclonal Antibody

Adiponectin Receptor 1 Antibody in Western Blot (WB)

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Adiponectin Receptor 1 Antibody (PA1-059) in WB

Western blot analysis of Adiponectin Receptor 1 was performed by loading 25 µg of rat skeletal muscle (lane 1), human skeletal muscle (lane 2) and HepG2 (lane 3) lysates onto an SDS polyacrylamide gel. Proteins were transferred to a PVDF membrane and blocked at 4ºC overnight. The membrane was probed with an Adiponectin Receptor 1 polyclonal antibody (Product # PA1-059) at a dilution of 1:500 overnight at 4°... View More

Adiponectin Receptor 1 Antibody in Immunocytochemistry, Immunohistochemistry (ICC/IF, IHC)

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产品信息

PA1-059

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:1,000

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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产品规格

种属反应

Human, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to residues R(20) E A D T V E L A E L G P L L E E K(37) of human Adiponectin Receptor 1.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS with 1mg/mL BSA

内含物

0.05% sodium azide

保存条件

-20°C, Avoid Freeze/Thaw Cycles

运输条件

Ambient (domestic); Wet ice (international)

AB_2221905

产品详细信息

PA1-059 detects human and rat adiponectin receptor 1.

PA1-059 has been successfully used in Western blot procedures. By Western blot, this antibody detects an ~42 kDa protein corresponding to human adiponectin receptor 1 from transfected hepatocytes.

The PA1-059 immunogen is a synthetic peptide corresponding to residues R(20) E A D T V E L A E L G P L L E E K(37) of human Adiponectin Receptor 1.

靶标信息

Adiponectin (also known as 30- kDa adipocyte complement-related protein; Acrp30) is a hormone secreted by adipocytes that acts as an antidiabetic and anti-atherogenic adipokine. Levels of adiponectin in the blood are decreased under conditions of obesity, insulin resistance and type 2 diabetes. Administration of adiponectin causes glucose-lowering effects and ameliorates insulin resistance in mice. Conversely, adiponectin-deficient mice exhibit insulin resistance and diabetes. This insulin-sensitizing effect of adiponectin seems to be mediated by an increase in fatty-acid oxidation through activation of AMP kinase and PPAR-. Here we report the cloning of complementary DNAs encoding adiponectin receptors 1 and 2 (AdipoR1 and AdipoR2) by expression cloning. AdipoR1 is abundantly expressed in skeletal muscle, whereas AdipoR2 is predominantly expressed in the liver. These two adiponectin receptors are predicted to contain seven transmembrane domains, but are structurally and functionally distinct from G-protein-coupled receptors. Expression of AdipoR1/R2 or suppression of AdipoR1/R2 expression by small-interfering RNA supports our conclusion that they serve as receptors for globular and full-length adiponectin, and that they mediate increased AMP kinase and PPAR- ligand activities, as well as fatty-acid oxidation and glucose uptake by adiponectin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Adiponectin receptor agonists inhibit leptin induced pSTAT3 and in vivo pancreatic tumor growth.

Oncotarget

Messaggio F,Mendonsa AM,Castellanos J,Nagathihalli NS,Gorden L,Merchant NB,VanSaun MN

PA1-059 was used in Immunohistochemistry-immunofluorescence to demonstrate that adiponectin receptor activation is a key regulator of pancreatic cancer growth.

Tue Oct 17 00:00:00 UTC 2017

Adiponectin receptor agonists inhibit leptin induced pSTAT3 and in vivo pancreatic tumor growth.

Oncotarget

Messaggio F,Mendonsa AM,Castellanos J,Nagathihalli NS,Gorden L,Merchant NB,VanSaun MN

PA1-059 was used in Immunohistochemistry-immunofluorescence to demonstrate that adiponectin receptor activation is a key regulator of pancreatic cancer growth.

Tue Oct 17 00:00:00 UTC 2017

Adiponectin receptor agonists inhibit leptin induced pSTAT3 and in vivo pancreatic tumor growth.

Oncotarget

Messaggio F,Mendonsa AM,Castellanos J,Nagathihalli NS,Gorden L,Merchant NB,VanSaun MN

PA1-059 was used in Immunohistochemistry-immunofluorescence to demonstrate that adiponectin receptor activation is a key regulator of pancreatic cancer growth.