Invitrogen

Alix Monoclonal Antibody (3A9)

Name: Alix Monoclonal Antibody (3A9)
Brand: Invitrogen
SKU: MA1-83977

高级验证

26个已发表图片

35 篇参考文献

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说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

Alix Antibody in Immunocytochemistry (ICC/IF)

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Alix Antibody (MA1-83977) in ICC/IF

Immunofluorescence analysis of Alix was performed using 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 15 minutes, and blocked with 2% BSA for 1 hour at room temperature. The cells were labeled with Alix Monoclonal Antibody (3A9) (Product # MA1-83977) at 5µg/mL in 0.1% BSA, incubated at 4 degree Celsius overnight and then with Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A28175) at a dilution of 1... View More

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Alix Antibody — Antibody specificity was demonstrated by siRNA mediated KD of target protein. HeLa cells were transfected with Alix siRNA and loss of signal was observed in Western Blot using Alix Monoclonal Antibody (3A9) (Product # MA1-83977). {KD}

产品信息

MA1-83977

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500

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免疫组化 (IHC)

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免疫组化（石蜡） (IHC (P))

Assay-dependent

免疫细胞化学 (ICC/IF)

5 µg/mL

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酶联免疫吸附实验 (ELISA)

Assay-dependent

查看1篇已发表文章 1篇已发表文章

免疫沉淀 (IP)

Assay-dependent

点杂交 (DB)

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亲和纯化 (AP)

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产品规格

种属反应

Human, Mouse, Rat, Xenopus

已发表种属

Bacteria, C. elegans, Human, Mouse, Rat

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

3A9

抗原

Alix fusion protein.

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2162469

产品详细信息

This product does not require protein digestion pre-treatment of paraffin sections. A ~100kD band is detected by Western blot.

Mouse anti ALIX antibody, clone 3A9 recognizes the apoptosis-linked gene-2 interacting protein X (ALIX), also known as Programmed cell death 6-interacting protein or Hp95.

靶标信息

Apoptotic linked-gene-product 2 (ALG-2) interacting protein X (ALIX) is a conserved adaptor protein which is ubiquitously expressed. Alix was originally reported to play a role in apoptosis but has recently been shown to be involved in other cellular mechanisms including endosomal sorting, endocytosis, viral budding and actin cytoskeleton assembly.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: ALG-2 interacting protein 1; ALG-2 interacting protein X; ALG-2-interacting protein 1; ALG-2-interacting protein X; Alg2-interacting protein 1; Alg2-interacting protein X; apoptosis-linked gene 2-interacting protein X; dopamine receptor interacting protein 4; E2F1-inducible protein; Eig2; Hp95; MGC17003; PDCD6-interacting protein; Programmed cell death 6-interacting protein; putative signal tranduction protein Xp95; Signal transduction protein Xp95

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基因别名: AI480591; AIP1; ALIX; AW544830; C76364; DRIP4; Eig2; HP95; KIAA1375; mKIAA1375; PDCD6IP; RGD1561176; XELAEV_18031317mg; xp95