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          Disclaimer

          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • Angiotensinogen Antibodies

          Invitrogen

          Angiotensinogen Polyclonal Antibody

          高级验证
          查看其他20个Angiotensinogen抗体

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          Cite Angiotensinogen Polyclonal Antibody

          • 抗体检测数据 (2)
          • 高级验证 (1)
          Angiotensinogen Antibody in Western Blot (WB)
          Group 53 Created with Sketch.
          Angiotensinogen Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          图: 1 / 3

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          Angiotensinogen Antibody (PA5-78740) in WB

          Western blot was performed using Anti-Angiotensinogen Rabbit Polyclonal Antibody (Product # PA5-78740) and a 53 kDa band corresponding to Angiotensinogen was observed in Mouse liver. Whole cell extracts (30 µg lysate) of Mouse liver (Lane 1), Rat liver (Lane 2), Mouse spleen (Lane 3) and Rat spleen (Lane 4) were electrophoresed using Novex® NuPAGE® 12 % Bis-Tris gel (Product # NP0342BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot ... View More {{ $ctrl.currentElement.advancedVerification.fullName }} 验证信息 View more
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          Angiotensinogen Antibody in Western Blot (WB)
          Angiotensinogen Antibody in Western Blot (WB)
          Angiotensinogen Antibody

          产品信息

          PA5-78740

          应用
          建议稀释比
          已发表文章

          免疫印迹 (WB)

          0.1-0.5 µg/mL
          -

          免疫组化(石蜡) (IHC (P))

          0.5-1 µg/mL
          -
          产品规格

          种属反应

          Mouse, Rat

          宿主/亚型

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          抗原

          E.coli-derived mouse Angiotensinogen recombinant protein (Position: D25-A115).
          查看免疫原

          偶联物

          Unconjugated Unconjugated Unconjugated

          形式

          Lyophilized

          浓度

          500 µg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS with 4mg trehalose

          内含物

          0.05mg sodium azide

          保存条件

          -20°C

          运输条件

          Ambient (domestic); Wet ice (international)

          RRID

          AB_2745856

          产品详细信息

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Positive Control - WB: mouse liver tissue. IHC: mouse kidney tissue.

          靶标信息

          The protein encoded by AGT, pre-angiotensinogen or angiotensinogen precursor, is expressed in the liver and is cleaved by the enzyme renin in response to lowered blood pressure. The resulting product, angiotensin I, is then cleaved by angiotensin converting enzyme (ACE) to generate the physiologically active enzyme angiotensin II. The protein is involved in maintaining blood pressure and in the pathogenesis of essential hypertension and preeclampsia. Mutations in this gene are associated with susceptibility to essential hypertension, and can cause renal tubular dysgenesis, a severe disorder of renal tubular development. Defects in this gene have also been associated with non-familial structural atrial fibrillation, and inflammatory bowel disease.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          篇参考文献 (0)

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          引用该产品

          生物信息学

          蛋白别名: angiotensin I; angiotensin II; angiotensin ll; Angiotensinogen; angiotensinogen (PAT); Serpin A8

          查看更多 收起

          基因别名: Agt; AI265500; Ang; AngI; AngII; ANRT; Aogen; PAT; Serpina8

          查看更多 收起

          UniProt ID: (Rat) P01015

          查看更多 收起

          Entrez Gene ID: (Rat) 24179, (Mouse) 11606

          查看更多 收起

          功能
          serine-type endopeptidase inhibitor activity hormone activity sodium channel regulator activity angiotensin receptor binding type 1 angiotensin receptor binding type 2 angiotensin receptor binding protease inhibitor
          参与通路
          ovarian follicle rupture blood vessel development branching involved in ureteric bud morphogenesis kidney development regulation of systemic arterial blood pressure by circulatory renin-angiotensin angiotensin mediated vasoconstriction involved in regulation of systemic arterial blood pressure renal response to blood flow involved in circulatory renin-angiotensin regulation of systemic arterial blood pressure renin-angiotensin regulation of aldosterone production regulation of renal output by angiotensin brain renin-angiotensin system renal system process angiotensin-mediated drinking behavior positive regulation of extracellular matrix constituent secretion cellular sodium ion homeostasis cell-matrix adhesion cell surface receptor signaling pathway G-protein coupled receptor signaling pathway activation of phospholipase C activity activation of NF-kappaB-inducing kinase activity female pregnancy aging excretion establishment of blood-nerve barrier regulation of blood pressure positive regulation of cell proliferation negative regulation of cell proliferation response to cold response to salt stress regulation of gene expression positive regulation of activation of JAK2 kinase activity positive regulation of endothelial cell migration positive regulation of cardiac muscle hypertrophy positive regulation of gene expression positive regulation of cholesterol esterification negative regulation of endopeptidase activity regulation of norepinephrine secretion positive regulation of phosphatidylinositol 3-kinase signaling artery smooth muscle contraction response to muscle activity involved in regulation of muscle adaptation negative regulation of angiogenesis extracellular matrix organization negative regulation of cell growth peristalsis positive regulation of cellular protein metabolic process positive regulation of superoxide anion generation positive regulation of peptidyl-serine phosphorylation negative regulation of tissue remodeling catenin import into nucleus regulation of renal sodium excretion positive regulation of renal sodium excretion positive regulation of multicellular organism growth vasoconstriction vasodilation hormone metabolic process drinking behavior regulation of apoptotic process positive regulation of catalytic activity positive regulation of MAPK cascade negative regulation of neuron apoptotic process positive regulation of nitric oxide biosynthetic process positive regulation of fatty acid biosynthetic process positive regulation of epidermal growth factor receptor signaling pathway positive regulation of blood pressure positive regulation of transcription, DNA-templated positive regulation of vasodilation positive regulation of organ growth astrocyte activation fibroblast proliferation positive regulation of fibroblast proliferation regulation of long-term neuronal synaptic plasticity smooth muscle cell proliferation cytokine secretion regulation of inflammatory response positive regulation of peptidyl-tyrosine phosphorylation smooth muscle cell differentiation negative regulation of neurotrophin TRK receptor signaling pathway stress-activated MAPK cascade regulation of calcium ion transport regulation of transmission of nerve impulse cell growth involved in cardiac muscle cell development positive regulation of protein tyrosine kinase activity ERK1 and ERK2 cascade uterine smooth muscle contraction cellular response to mechanical stimulus positive regulation of branching involved in ureteric bud morphogenesis positive regulation of protein kinase C activity regulation of extracellular matrix assembly positive regulation of membrane hyperpolarization positive regulation of gap junction assembly regulation of cardiac conduction positive regulation of extrinsic apoptotic signaling pathway regulation of heart rate
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