MUbio

Annexin V Monoclonal Antibody (RUU-WAC2A)

Name: Annexin V Monoclonal Antibody (RUU-WAC2A)
Brand: MUbio
SKU: MUB0106P

查看其他27个Annexin V抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

产品信息

MUB0106P

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:100-1:500

免疫组化（石蜡） (IHC (P))

1:50-1:100

免疫组化（冰冻） (IHC (F))

1:50-1:100

免疫细胞化学 (ICC/IF)

Assay-dependent

酶联免疫吸附实验 (ELISA)

Assay-dependent

产品规格

种属反应

Human, Zebrafish

宿主/亚型

Mouse / IgG1

分类

Monoclonal

类型

Antibody

克隆号

RUU-WAC2A

抗原

annexin V

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

产品详细信息

RUU-WAC2A reacts anti Human recombinant and endogenous annexin A5. It shows no cross reactivity with Annexins I, II, III, IV, VI, VII and VIII in ELISA or in immunoprecipitation. It does not recognise Monkey and rat Annexin V in ELISA.

For immunohistochemistry of paraffin sections a pretreatment step with 2% amino-propyl-triethoxysilan (in 100% ethanol) and 0,2 % BSA is recommended. The sections are heated for 15 minutes at 100C in a citrate buffer (10 mM trisodium citrate, pH 6,0). RUU-WAC2a inhibits annexin A5 binding to anionic phospholipid-containing vesicles, activated platelets and apoptotic cells for more than 95%.

The antibody may be stored at 4°C. For prolonged storage prepare appropriate aliquots and store at or below -20°C. Prior to use, an aliquot is thawed slowly in the dark at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used the same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance or the concentration of the product.

靶标信息

The protein encoded by this gene belongs to the annexin family of calcium-dependent phospholipid binding proteins some of which have been implicated in membrane-related events along exocytotic and endocytotic pathways. Annexin 5 is a phospholipase A2 and protein kinase C inhibitory protein with calcium channel activity and a potential role in cellular signal transduction, inflammation, growth and differentiation. Annexin 5 has also been described as placental anticoagulant protein I, vascular anticoagulant-alpha, endonexin II, lipocortin V, placental protein 4 and anchorin CII. The gene spans 29 kb containing 13 exons, and encodes a single transcript of approximately 1.6 kb and a protein product with a molecular weight of about 35 kDa.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

篇参考文献 (0)

您是否在文献中引用过该产品？请点击下方按钮邮件告知我们。

引用该产品

生物信息学

蛋白别名: Anchorin CII; Annexin A5; Annexin V; Annexin-5; Calphobindin I; CBP-I; CPB-I; Endonexin II; epididymis secretory protein Li 7; Lipocortin V; PAP-I; Placental anticoagulant protein 4; Placental anticoagulant protein I; PP4; Thromboplastin inhibitor; VAC-alpha; Vascular anticoagulant-alpha

查看更多收起

基因别名: ANX5; ANXA5; ENX2; HEL-S-7; PP4; RPRGL3

查看更多收起

UniProt ID: (Human) P08758

查看更多收起

Entrez Gene ID: (Human) 308

查看更多收起

功能

phospholipase inhibitor activity calcium-transporting ATPase activity calcium ion binding protein binding phospholipid binding calcium-dependent phospholipid binding peptide hormone binding receptor tyrosine kinase binding calcium-binding protein

参与通路