Invitrogen
This Antibody was verified by Relative expression to ensure that the antibody binds to the antigen stated.
应用 | 建议稀释比 | 已发表文章 |
---|---|---|
免疫印迹 (WB) |
1:500-1:2,000 | - |
免疫组化(石蜡) (IHC (P)) |
1:20-1:100 | - |
酶联免疫吸附实验 (ELISA) |
1 µg/mL | - |
免疫沉淀 (IP) |
Assay-dependent | - |
放射免疫测定 (RIA) |
Assay-dependent | - |
产品规格 | |
---|---|
种属反应 |
Human, Mouse |
宿主/亚型 |
Mouse / IgG1 |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
532 |
抗原 |
Purified human plasma Apo A-1 |
偶联物 |
Unconjugated |
形式 |
Liquid |
浓度 |
5 mg/mL |
纯化类型 |
Protein A |
保存液 |
PBS, pH 7.4 |
内含物 |
0.09% sodium azide |
保存条件 |
Maintain refrigerated at 2-8°C for up to 6 months. For long term storage store at -20°C |
RRID |
AB_11152905 |
By sandwich ELISA, MIA1405 can be used as a detection antibody with Product # 710263 or # 701239 as a coating antibody, to generate a matched pair. Using these matched pairs, recombinant human Apo A-1, but not recombinant mouse Apo A-1, was detected. MIA1405 can be used to detect Apo A-1 from serum samples. To increase sensitivity of sandwich ELISAs with MIA1405, a biotinylated detection antibody followed by Streptavidin-HRP is recommended.
By Western blot, MIA1405 detects recombinant human Apo A-1, but not recombinant mouse Apo A-1. MIA1405 is also recommended for detecting endogenous Apo A-1 by Western blot.
MIA1405 was formerly sold as a Seradyn product.
Apolipoprotein A-1 (apo A-1) in human plasma is a major protein component of high density lipoprotein (HDL). The molecular mass is 24 kDa. Apo A-1 is a cofactor for enzymes involved in the normal turnover of cholesterol and is produced in the liver. Plasma concentration is 270-380 mg/100 mL.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: 1-Sep; 2-Sep; apo A-1; APO A1; APO I; Apo-AI; ApoAI; Apolipoprotein; Apolipoprotein A-I; Apolipoprotein A-I(1-242); Apolipoprotein A1; Apolipoprotein AI; MGC102525; MGC117399; MGC124929; ProapoA-I; Proapolipoprotein A-I; Truncated apolipoprotein A-I; Udpgt; Ugt1; Ugt1a7
基因别名: Alp-1; apo(a); apo-AI; Apoa-1; apoA-I; APOA1; Brp-14; Ltw-1; Lvtw-1; Sep-1; Sep-2; Sep2
UniProt ID: (Human) P02647, (Mouse) Q00623
Entrez Gene ID: (Human) 335, (Mouse) 11806
分子生物学功能:
apolipoprotein