Invitrogen

Aquaporin 0 Polyclonal Antibody

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Aquaporin 0 Antibody in Immunohistochemistry (IHC) — Immunohistochemical staining of Aquaporin 0 in human liver shows moderate cytoplasmic positivity in a subset of hepatocytes. Samples were probed using an Aquaporin 0 Polyclonal Antibody (Product # PA5-64958).

Aquaporin 0 Antibody in Western Blot (WB) — Western blot was performed using Anti-Aquaporin 0 Polyclonal Antibody (Product # PA5-64958) and bands ranging from 60 kDa through 200 kDa corresponding to Aquaporin 0 were observed in the tissues tested. Tissue extracts (30 µg lysate) of Mouse Eye (Lane 1) and Rat Eye (Lane 2) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot was probed with the primary antibody (1:1000 dilution) and detected by chemiluminescence with Goat anti-Rabbit IgG (H+L) Superclonal™ Recombinant Secondary Antibody, HRP (Product # A27036, 1:4000 dilution) using the iBright FL 1000 (Product # A32752). Chemiluminescent detection was performed using Novex® ECL Chemiluminescent Substrate Reagent Kit (Product # WP20005).

产品信息

PA5-64958

应用	建议稀释比	已发表文章
免疫组化 (IHC)	1:500-1:1,000	-

产品规格
种属反应	Human
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant Human Aquaporin 0 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	0.05 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.2, with 40% glycerol
内含物	0.02% sodium azide
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2662767

产品详细信息

Immunogen sequence: RLKSISERLSV LKGAKPDVSN GQPEVTGEPV ELNTQAL

Highest antigen sequence identity to the following orthologs - mouse 82%, rat 82%.

靶标信息

AQP0 is the most abundant endogenous protein in the plasma membrane of lens fiber cells where it functions not only as a water pore, but it is also involved in fiber-fiber adhesion and is crucial for fiber cell structure and organization. AQP0 contains an additional pore constriction, not seen in any other aquaporin structures, which may be responsible for pore gating. The closed AQP0 pore holds just three water molecules, which are spaced too far apart to form hydrogen bonds with each other. The C-terminal domain of AQP0 undergoes extensive post-translational modification, including many truncations, during lens aging due to the actions of m-calpain, proteases or non-enzymatic mechanisms. These truncation sites may be involved in the development of cataracts.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: aquaporin 0; Aquaporin-0; Aquaporin0; Lens fiber major intrinsic protein; MIP26; MP26

基因别名: AQP0; CTRCT15; LIM1; MIP; MIP26; MP26

UniProt ID: (Human) P30301

Entrez Gene ID: (Human) 4284

分子生物学功能: transporter

功能

lens development in camera-type eye water transport visual perception cellular water homeostasis canalicular bile acid transport glycerol transport ion transmembrane transport positive regulation of cell adhesion response to stimulus protein homotetramerization gap junction-mediated intercellular transport

参与通路

structural constituent of eye lens transporter activity gap junction channel activity calmodulin binding water channel activity glycerol channel activity

Aquaporin 0 Polyclonal Antibody

产品信息

PA5-64958

免疫组化 (IHC)

种属反应

宿主/亚型

分类

类型

抗原

偶联物

形式

浓度

纯化类型

保存液

内含物

保存条件

RRID

产品详细信息

靶标信息

篇参考文献 (0)

生物信息学

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