Invitrogen

Arp2 Polyclonal Antibody

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Arp2 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of Arp2 was performed in U2OS cells using Arp2 Polyclonal Antibody (Product # PA5-96410).

Arp2 Antibody in Immunohistochemistry (IHC) — Immunohistochemistry analysis of Arp2 in paraffin-embedded human uterine cancer using Arp2 Polyclonal Antibody (Product # PA5-96410) at a dilution of 1:100.

Arp2 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extract (30 µg lysate) of HeLa (Lane 1), Hep G2 (Lane 2), THP-1 (Lane 3), and Mouse brain (Lane 4). The blot was probed with Anti-ACTR2 Polyclonal Antibody (Product # PA5-96410, 1:1000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 45 kDa band corresponding to ACTR2 was detected in all cell lines and tissue tested.

产品信息

PA5-96410

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:2,000	-
免疫组化（石蜡） (IHC (P))	1:50 - 1:200	-
免疫细胞化学 (ICC/IF)	1:50 - 1:200	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ACTR2.
偶联物	Unconjugated
形式	Liquid
浓度	1.61 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7.3, with 50% glycerol
内含物	0.02% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2808212

产品详细信息

Immunogen sequence: MDSQGRKVVV CDNGTGFVKC GYAGSNFPEH IFPALVGRPI IRSTTKVGNI EIKDLMVGDE ASELRSMLEV NYPMENGIVR NWDDMKHLWD YTFGPEKLNI DTRNCKILLT EPPMNPTKNR EKIVEVMFET YQFSGVYVAI QAVLTLYAQG LLTGVVVDSG; Positive Samples: THP-1, 293T, HepG2, HeLa, C6, Mouse spleen, Mouse lung, Mouse brain, Rat kidney; Cellular Location: Cell projection, Cytoplasm, cytoskeleton

靶标信息

ARP2 (actin-related protein 2) is an ATP-binding component of the ARP2/3 complex - a multiprotein complex that mediates actin polymerization upon stimulation by the nucleation-promoting factor (NPF). The ARP2/3 complex mediates the formation of branched actin networks in the cytoplasm and provides the force for cell motility. The ARP2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA.

仅用于科研。不用于诊断过程。未经明确授权不得转售。