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FIGURE: 1 / 6
Arp2 Antibody (PA5-96410) in WB
Western blot analysis of Arp2 in extracts of various cell lines. Samples were incubated with Arp2 Polyclonal antibody (Product # PA5-96410) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Product Details
PA5-96410
Applications
Tested Dilution
Publications
产品规格
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
Recombinant fusion protein containing a sequence corresponding to amino acids 1-160 of human ACTR2.
Conjugate
Unconjugated
形式
Liquid
浓度
0.3 mg/mL
纯化类型
Affinity Chromatography
保存液
PBS, pH 7.3, with 50% glycerol
内含物
0.02% sodium azide
保存条件
-20° C, Avoid Freeze/Thaw Cycles
RRID
AB_2808212
Product Specific Information
Immunogen sequence: MDSQGRKVVV CDNGTGFVKC GYAGSNFPEH IFPALVGRPI IRSTTKVGNI EIKDLMVGDE ASELRSMLEV NYPMENGIVR NWDDMKHLWD YTFGPEKLNI DTRNCKILLT EPPMNPTKNR EKIVEVMFET YQFSGVYVAI QAVLTLYAQG LLTGVVVDSG; Positive Samples: THP-1, 293T, HepG2, HeLa, C6, Mouse spleen, Mouse lung, Mouse brain, Rat kidney; Cellular Location: Cell projection, Cytoplasm, cytoskeleton
Target Information
ARP2 (actin-related protein 2) is an ATP-binding component of the ARP2/3 complex - a multiprotein complex that mediates actin polymerization upon stimulation by the nucleation-promoting factor (NPF). The ARP2/3 complex mediates the formation of branched actin networks in the cytoplasm and provides the force for cell motility. The ARP2/3 complex also promotes actin polymerization in the nucleus, thereby regulating gene transcription and repair of damaged DNA.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: Actin-like protein 2; Actin-related protein 2; ARP2 actin-related protein 2 homolog
Gene Aliases: 4921510D23Rik; AA409782; ACTR2; ARP2; D6Ertd746e
UniProt ID: (Human) P61160, (Rat) Q5M7U6, (Mouse) P61161
Entrez Gene ID: (Human) 10097, (Rat) 289820, (Mouse) 66713
Molecular Function:
actin and actin related protein
actin or actin-binding cytoskeletal protein
cytoskeletal protein
movement of cell or subcellular component
establishment or maintenance of cell polarity
asymmetric cell division
meiotic chromosome movement towards spindle pole
cytosolic transport
meiotic cytokinesis
Arp2/3 complex-mediated actin nucleation
Fc-gamma receptor signaling pathway involved in phagocytosis
cilium assembly
ephrin receptor signaling pathway
spindle localization
actin cytoskeleton organization
meiotic cell cycle
actin filament organization
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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