Invitrogen

Ataxin 3 Polyclonal Antibody

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated. 查看细节

3个已发表图片

2篇引用文献

查看其他12个Ataxin 3抗体

说明书

Protocols

技术支持

说明书

Protocols

技术支持

Product Image Gallery

Ataxin 3 Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of Ataxin 3 was performed in SK N SH cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Ataxin 3 Polyclonal Antibody (Product # PA5 22369) diluted at 1:250. Blue: Hoechst 33342 staining.

Ataxin 3 Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemical analysis of Ataxin 3 in paraffin-embedded H661 xenograft using an Ataxin 3 polyclonal antibody (Product # PA5-22369) at a dilution of 1:500.

Ataxin 3 Antibody in Western Blot (WB) — Western blot analysis was performed on whole cell extract (30 µg lysate) of HCT116 (Lane 1), HCT116 treated with thapsigargin (2uM, 6 hrs) (Lane 2), SH-SY5Y (Lane 3), and U-87MG (Lane 4). The blot was probed with Anti-Ataxin 3 Polyclonal Antibody (Product # PA5-22369, 1:3000 dilution) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.25 µg/ml, 1:4000 dilution). A 42 kDa band corresponding to Ataxin 3 was detected in cell lines tested.

Ataxin 3 Antibody — Antibody specificity was demonstrated by CRISPR-Cas9 mediated knockout of target protein. A loss of signal was observed for target protein in Ataxin3 knockout (KO) cell line compared to control cell line using Ataxin 3 Polyclonal Antibody (Product # PA5-22369). {KO}

Ataxin 3 Antibody in Immunohistochemistry (IHC) — Figure 6 Protein aggregation in renal proximal tubules after angiotensin II treatment. Angiotensin was administered to rats to increase cellular protein turnover in proximal tubules, and aggregation-prone protein content was analyzed by immunohistochemistry. Angiotensin II was elevated by either infusion from subcutaneous minipumps or by adrenalectomy with glucocorticoid substitution. (A) Representative example of the RPL22/27 immunostaining in the kidney cortex from a control and angiotensin II (AngII)-treated rats, as indicated. PT denotes proximal tubules, while DT marks distal tubules and connecting tubules. (B) Similar RPL22/27 staining in sections from dexamethasone-treated adrenalectomized rats (ADX) and from similar rats with aldosterone and dexamethasone coadministration (ADX+Aldo). (C) Micrographs comparing keratin 5 immunoreactivity in renal cortex from control and angiotensin II-treated rats. (D) Immunolabeling for ataxin-3 in the angiotensin II versus vehicle administration model, as indicated. (E) Similar staining in model of dexamethasone-corrected adrenalectomy with and without angiotensin II administration (ADX+AngII), as indicated.

产品细节

Applications

Tested Dilution

Publications

免疫印迹 (WB)

1:500-1:3,000

View 1 publication 1 publication

免疫组化 (IHC)

View 1 publication 1 publication

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse

已发表种属

Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fragment corresponding to a region within amino acids 1 and 245 of Ataxin 3 (Uniprot ID#P54252)

偶联物

Unconjugated

形式

Liquid

浓度

0.36 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 20% glycerol, 1% BSA

内含物

0.025% Proclin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

RRID

AB_11152091

产品详细信息

Recommended positive controls: SK-N-SH, SK-N-AS, mouse brain, human Ataxin3-transfected 293T.

Predicted reactivity: Mouse (96%), Rat (96%), Zebrafish (85%), Pig (99%), Chicken (89%), Sheep (98%), Bovine (98%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Machado-Joseph disease is an autosomal dominant neurologic disorder, and is now known to be the same as previously described spinocerebellar ataxia-3. MJD protein (Ataxin-3) contains (CAG)n repeats in the coding region, and the expansion of these repeats from the normal 13-36 to 68-79 is the cause of Machado-Joseph disease. There is a negative correlation between the age of onset and CAG repeat numbers. This protein interacts with key regulators (CBP, p300 and PCAF) of transcription and represses transcription, and also acts as a histone-binding protein that regulates transcription. MJD is a deubiquitinating enzyme.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

生物信息学

蛋白别名: ataxin 3 variant an; ataxin 3 variant ao; ataxin 3 variant at; ataxin 3 variant e; ataxin 3 variant h; ataxin 3 variant m; ataxin 3 variant r; ataxin 3 variant ref; ataxin 3 variant y; Ataxin-3; ataxin3; josephin; Machado-Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3); Machado-Joseph disease (spinocerebellar ataxia 3, olivopontocerebellar ataxia 3, autosomal dominant, ataxin 3) homolog; Machado-Joseph disease protein 1; Machado-Joseph disease protein 1 homolog; olivopontocerebellar ataxia 3; spin; Spinocerebellar ataxia type 3 protein

基因别名: 2210008M02Rik; AI463012; AI647473; AT3; ataxin-3; ATX3; ATXN3; JOS; MJD; MJD1; SCA3

UniProt ID: (Human) P54252, (Mouse) Q9CVD2

Entrez Gene ID: (Human) 4287, (Mouse) 110616

分子生物学功能: DNA metabolism protein chromatin/chromatin-binding, or -regulatory protein