Invitrogen

Aurora B Polyclonal Antibody

Aurora B Antibody in Immunocytochemistry (ICC/IF)

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Aurora B Antibody (36-5200) in ICC/IF

Immunofluorescent analysis of Aurora B Antibody was done on 70% confluent log phase HeLa cells. The cells were fixed with 4% paraformaldehyde for 15 minutes, permeabilized with 0.25% Triton™ X-100 for 10 minutes, and blocked with 5% BSA for 1 hour at room temperature. The cells were labeled with Aurora B Antibody (Product # 36-5200) at 1µg/mL in 1% BSA and incubated for 3 hours at room temperature and then labeled with Alexa Fluor 488 Goat Anti-Rabbit IgG Secondary Antibody (Product # A-11008) at a dilution of 1:400 for 45 minutes at room ... View More

Aurora B Antibody in Immunohistochemistry (IHC)

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产品信息

36-5200

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1 µg/mL

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免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

1 µg/mL

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免疫沉淀 (IP)

Assay-dependent

产品规格

种属反应

Human

已发表种属

Bovine, Human, Sheep

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide derived from the N-terminal region of the human Aurora B protein.

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.25 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7.4

内含物

0.1% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2533266

靶标信息

The Aurora kinases associate with microtubules during chromosome movement and segregation. AURKB (Aurora B) localizes to microtubules near kinetochores, specifically to the specialized microtubules called K-fibers. This complex functions in chromosome alignment and separation, histone modification, and cytokinesis. CPC also includes three non-enzymatic subunits known as the inner centromere protein (INCENP), survivin, and borealin, which determine the activity, localization, stability, and possibly even the substrate specificity of AurB. Two CPCs exist during mitosis: one containing all four members and another consisting of INCENP and AurB. Quaternary CPC functions during chromosome alignment and cytokinesis, whereas the INCENP-AurB complex may be responsible for modifying histone H3. AurB is overexpressed in a number of tumours, including primary breast and colon tumour samples. Its expression in tumours seems to be linked with Aurora-A kinase, suggesting a feedback mechanism between the two proteins.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Dual roles of oxostephanine as an Aurora kinase inhibitor and angiogenesis suppressor.

International journal of molecular medicine

Tran TT,Vu LB,Nguyen HQ,Pham HB,Do XT,Than UTT,Pham TT,Do LD,Le KT,Nguyen TP,Hoang MT

36-5200 was used in Immunocytochemistry to demonstrate that oxostephanine plays dual roles in inhibiting Aurora kinase activity and angiogenesis. Thus, it may have potential for use as a drug in cancer treatment.

Tue Nov 01 00:00:00 UTC 2022

lncRNA ELFN1-AS1 enhances the progression of colon cancer by targeting miR-4270 to upregulate AURKB.

Open medicine (Warsaw, Poland)

Peng S,Luo Y,Chen L,Dai K,Wang Q

36-5200 was used in Western Blotting to show that the ELFN1-AS1/miR-4270/AURKB axis facilitates colon cancer (CC) tumorigenesis; therefore, targeting this axis might be a promising intervention in preventing CC progression.

Sat Dec 24 00:00:00 UTC 2022

Artemisinin Mediates Its Tumor-Suppressive Activity in Hepatocellular Carcinoma Through Targeted Inhibition of FoxM1.

Frontiers in oncology

Nandi D,Cheema PS,Singal A,Bharti H,Nag A

Published figure using Aurora B polyclonal antibody (Product # 36-5200) in Western Blot

Wed Dec 15 00:00:00 UTC 2021

USP48 Governs Cell Cycle Progression by Regulating the Protein Level of Aurora B.

International journal of molecular sciences

Antao AM,Kaushal K,Das S,Singh V,Suresh B,Kim KS,Ramakrishna S

Published figure using Aurora B polyclonal antibody (Product # 36-5200) in Western Blot

Sat Aug 07 00:00:00 UTC 2021

USP48 Governs Cell Cycle Progression by Regulating the Protein Level of Aurora B.

International journal of molecular sciences

Antao AM,Kaushal K,Das S,Singh V,Suresh B,Kim KS,Ramakrishna S

Published figure using Aurora B polyclonal antibody (Product # 36-5200) in Western Blot

Sat Aug 07 00:00:00 UTC 2021

Nucleostemin rejuvenates cardiac progenitor cells and antagonizes myocardial aging.

Journal of the American College of Cardiology

Hariharan N,Quijada P,Mohsin S,Joyo A,Samse K,Monsanto M,De La Torre A,Avitabile D,Ormachea L,McGregor MJ,Tsai EJ,Sussman MA

Published figure using Aurora B polyclonal antibody (Product # 36-5200) in Western Blot

Tue Jan 20 00:00:00 UTC 2015

Aurora kinase inhibitors reveal mechanisms of HURP in nucleation of centrosomal and kinetochore microtubules.

Proceedings of the National Academy of Sciences of the United States of America

Wu JM,Chen CT,Coumar MS,Lin WH,Chen ZJ,Hsu JT,Peng YH,Shiao HY,Lin WH,Chu CY,Wu JS,Lin CT,Chen CP,Hsueh CC,Chang KY,Kao LP,Huang CY,Chao YS,Wu SY,Hsieh HP,Chi YH

36-5200 was used in western blot to identify and test Aurora kinases inhibitors for anti-tumor activity.

Tue May 07 00:00:00 UTC 2013

Aurora B expression in post-puberal testicular germ cell tumours.

Journal of cellular physiology

Esposito F,Libertini S,Franco R,Abagnale A,Marra L,Portella G,Chieffi P

Published figure using Aurora B polyclonal antibody (Product # 36-5200) in Immunohistochemistry

Sun Nov 01 00:00:00 UTC 2009

Aurora B expression in post-puberal testicular germ cell tumours.

Journal of cellular physiology

Esposito F,Libertini S,Franco R,Abagnale A,Marra L,Portella G,Chieffi P

Published figure using Aurora B polyclonal antibody (Product # 36-5200) in Immunohistochemistry

Sun Nov 01 00:00:00 UTC 2009

Aurora B expression in post-puberal testicular germ cell tumours.

Journal of cellular physiology

Esposito F,Libertini S,Franco R,Abagnale A,Marra L,Portella G,Chieffi P

Published figure using Aurora B polyclonal antibody (Product # 36-5200) in Immunohistochemistry

Sun Nov 01 00:00:00 UTC 2009

Aurora B expression in post-puberal testicular germ cell tumours.

Journal of cellular physiology

Esposito F,Libertini S,Franco R,Abagnale A,Marra L,Portella G,Chieffi P

Published figure using Aurora B polyclonal antibody (Product # 36-5200) in Immunohistochemistry

Sun Nov 01 00:00:00 UTC 2009

Aurora B expression correlates with aggressive behaviour in glioblastoma multiforme.

Journal of clinical pathology

Zeng WF,Navaratne K,Prayson RA,Weil RJ

36-5200 was used in immunocytochemistry, immunohistochemistry - paraffin section, and western blot to elucidate the correlation between aggressive behaviour in glioblastoma multiforme and aurora B expression

Thu Feb 01 00:00:00 UTC 2007

生物信息学

蛋白别名: AIM-1; ARK-2; Aurora 1; Aurora kinase B; aurora kinase B-Sv1; aurora kinase B-Sv2; Aurora- and IPL1-like midbody-associated protein 1; aurora-1; aurora-B; aurora-related kinase 2; Aurora/IPL1-related kinase 2; protein phosphatase 1, regulatory subunit 48; serine/threonine kinase 12; Serine/threonine-protein kinase 12; Serine/threonine-protein kinase 5; Serine/threonine-protein kinase aurora-B; STK-1

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基因别名: AIK2; AIM-1; AIM1; AIRK2; ARK2; AurB; AURKB; aurkb-sv1; aurkb-sv2; IPL1; PPP1R48; STK1; STK12; STK5

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UniProt ID: (Human) Q96DV5

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Entrez Gene ID: (Human) 9212

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功能

protein serine/threonine kinase activity protein serine/threonine/tyrosine kinase activity protein binding ATP binding histone serine kinase activity metal ion binding non-receptor serine/threonine protein kinase

参与通路