Invitrogen

Axl Polyclonal Antibody

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Axl Antibody in Immunohistochemistry (Paraffin) (IHC (P)) — Immunohistochemistry analysis of Axl in paraffin-embedded rat prostate. Sample was incubated with Axl polyclonal antibody (Product # PA5-77874) using a dilution of 1:1000.

Axl Antibody in Western Blot (WB) — Western blot analysis of Axl in A) 293T whole cell lysate, B) whole cell lysate of human AXL-transfected 293T cells using 30 µg of protein. Samples were separated with 7.5% SDS-PAGE and incubated with Axl polyclonal antibody (Product # PA5-77874) using a dilution of 1:5000.

产品信息

PA5-77874

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:1,000-1:10,000	-
免疫组化（石蜡） (IHC (P))	1:100-1:1,000	-

产品规格
种属反应	Human, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Recombinant protein encompassing a sequence within the N-terminus region of human AXL. The exact sequence is proprietary.
偶联物	Unconjugated
形式	Liquid
浓度	0.92 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS, pH 7, with 20% glycerol
内含物	0.025% Proclin 300
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2735508

产品详细信息

Positive Control: human AXL-transfected 293T

Predicted Reactivity: Mouse (85%), Pig (81%)

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Axl is a member of the TAM receptor family, which also includes also MerTK and TYRO3. Axl contains protein kinase activity and is activated by dimerization and auto-phosphorylation upon ligand binding. In response to its ligands, Protein S and GAS6, Axl activates anti-inflammatory pathways (through SOCS-1 and SOCS-3), which limits TLR and cytokine signaling. This results in dampened inflammatory responses in macrophages and DCs. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: AXL oncogene; AXL transforming sequence/gene; AZF1; oncogene AXL; sAXL; soluble AXL; Tyrosine-protein kinase receptor UFO

基因别名: ARK; AXL; JTK11; Tyro7; UFO

UniProt ID: (Human) P30530

Entrez Gene ID: (Human) 558, (Rat) 308444

分子生物学功能: transmembrane signal receptor

neuron migration natural killer cell differentiation positive regulation of cytokine-mediated signaling pathway blood vessel remodeling phagocytosis inflammatory response signal transduction spermatogenesis peptidyl-tyrosine phosphorylation forebrain cell migration platelet activation cellular response to extracellular stimulus negative regulation of interferon-gamma production negative regulation of tumor necrosis factor production positive regulation of natural killer cell differentiation secretion by cell erythrocyte homeostasis substrate adhesion-dependent cell spreading cellular response to interferon-alpha ovulation cycle apoptotic cell clearance protein kinase B signaling negative regulation of neuron apoptotic process innate immune response viral entry into host cell vascular endothelial growth factor receptor signaling pathway cell maturation positive regulation of pinocytosis negative regulation of lymphocyte activation positive regulation of protein kinase B signaling vagina development cellular response to lipopolysaccharide dendritic cell differentiation negative regulation of dendritic cell apoptotic process immune system process protein phosphorylation enzyme linked receptor protein signaling pathway cell proliferation phosphorylation cell migration cell differentiation organ regeneration tissue regeneration negative regulation of apoptotic process tissue remodeling cellular response to hydrogen peroxide

参与通路

virus receptor activity phosphatidylserine binding protein tyrosine kinase activity transmembrane receptor protein tyrosine kinase activity protein binding ATP binding protein heterodimerization activity nucleotide binding protein kinase activity kinase activity transferase activity transferase activity, transferring phosphorus-containing groups growth factor binding myosin heavy chain binding phosphatidylinositol 3-kinase binding