Invitrogen

Axl Recombinant Mouse Monoclonal Antibody (DS7HAXL), Alexa Fluor™ Plus 488

Name: Axl Recombinant Mouse Monoclonal Antibody (DS7HAXL), Alexa Fluor\u2122 Plus 488
Brand: Invitrogen
SKU: 750032MP488

高级验证

查看其他62个AXL抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)
高级验证 (1)

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Axl Antibody (750032MP488) in Flow

Flow cytometry analysis of Axl Recombinant Mouse Monoclonal Antibody (DS7HAXL), Alexa Fluor™ Plus 488 (Product # 750032MP488) was performed using U-87 MG and SH-SY5Y cells. Cells were stained with 0.5 µg of the Axl Recombinant Mouse Monoclonal Antibody (DS7HAXL), Alexa Fluor™ Plus 488 (Product # 750032MP488). Higher expression of Axl was observed in U-87 MG (pink histogram) in comparison to SH-SY5Y (blue histogram). Viable cells were used for analysis as determined by LIVE/DEAD™ Fixable Violet Dead Cell Stain Kit (Product # L34964). Flow c... View More

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Axl Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. Axl Recombinant Mouse Monoclonal Antibody (DS7HAXL), Alexa Fluor™ Plus 488 (Product # 750032MP488) specifically detects Axl expression in U-87 MG cells (pink histogram) while lower expression of Axl is seen in SH-SY5Y cells (blue histogram). {RE}

产品信息

750032MP488

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.5 µg/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

Expression System

Expi293

分类

Recombinant Monoclonal

类型

Antibody

克隆号

DS7HAXL

偶联物

Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488 Alexa Fluor™ Plus 488

激发/发射光谱

493/518 nm 查看光谱

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

proprietary buffer, pH 6.3-7.3

内含物

0.008% Bromonitrodioxane, 0.008% Methylisothiazolone

保存条件

4°C, store in dark, DO NOT FREEZE!

运输条件

Wet ice

产品详细信息

Alexa Fluor™ Plus recombinant antibodies are conjugated using new, proprietary dye chemistry so you can generate stunning data. Alexa Fluor™ Plus antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor™ Plus antibodies provide brighter signal compared to leading Alexa Fluor™ antibodies, providing you with better signal-to-noise for your critical experiments. These antibodies show better specificity and lot-to-lot consistency as these are recombinant antibodies, generated by cloning specific genes for the desired antibodies into an expression vector and expressed in vitro.

Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

Excitation: 494 nm; Emission: 519 nm; Laser: Blue Laser

Filtration: 0.2 µm post-manufacturing filtered.

靶标信息

AXL is a chronic myelogenous leukemia-associated oncogene that is also associated with colon cancer and melanoma. The AXL protein is a receptor tyrosine kinase that transduces signals from the extracellular matrix into the cytoplasm by binding growth factors like vitamin K-dependent protein growth-arrest-specific gene 6, and is involved in the stimulation of cell proliferation. TAM receptors are also involved in phagocytosis of apoptotic cells. Mice lacking all three TAM receptors have several degenerative phenotypes linked to inefficient removal of apoptotic cells and membranes (e.g., in the retina and the male reproductive tract) and develop a severe autoimmune phenotype akin to systemic lupus erythematosus, including the production of broad spectrum auto-antibodies. In addition, Axl may function as a putative entry receptor for filoviruses. Axl is also used to identify a specific subpopulation of human blood dendritic cells, also referred to as Siglec 6+/Axl+ dendritic cells. Cellular expression of Axl can be upregulated by TLR ligands, such as LPS or poly I:C. Soluble Axl is generated by proteolytic cleavage of the membrane form. Increased plasma levels may be indicative of inflammation and cancer.

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