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| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
0.1 µg/mL | - |
免疫细胞化学 (ICC/IF) |
- | 查看1篇已发表文章 1篇已发表文章 |
流式细胞分析 (Flow) |
2.5 µg/1x10^6 cells | - |
| 产品规格 | |
|---|---|
种属反应 |
Mouse |
已发表种属 |
Human |
宿主/亚型 |
Goat / IgG |
分类 |
Polyclonal |
类型 |
Antibody |
抗原 |
Mouse myeloma cell line NS0-derived recombinant mouse B7-H4 Phe29-Pro258 |
偶联物 |
Unconjugated |
形式 |
Lyophilized |
浓度 |
0.2 mg/mL |
纯化类型 |
Antigen affinity chromatography |
保存液 |
PBS with 5% trehalose |
内含物 |
No Preservative |
保存条件 |
-20° C, Avoid Freeze/Thaw Cycles |
RRID |
AB_2607079 |
In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant mouse (rm) B7-1, rmB7-2, rmB7-H1, rmB7-H2, rmB7-H3, and rmPD-L2 is observed.
Reconstitute at 0.2 mg/mL in sterile PBS.
B7-H4 (B7x, VTCN1) a costimulatory protein which is reported to function as a negative regulator of T-cell mediated immunity. Although B7-H4 binds an unknown receptor, it is thought to deliver an inhibitory signal to T-cells preventing their proliferation, cell cycle progression and interleukin-2 production. B7-H4 deficient mice are only minimally affected; suggesting B7-H4 is important in the fine tuning of the T-cell mediated immune response. B7-H4 is expressed on activated T-cells, B-cells, monocytes and dendritic cells. Aberrant expression has been associated with cancers of the lung, breast and ovary in humans. B7-H4 is expressed primarily on the membrane of lymphoid cells, and as an immunoinhibitory protein that interacts with receptors on the surface of T lymphocytes, it is involved in mediating cellular and humoral immune responses. Overexpression of B7-H4 is associated with certain malignancies, including ovarian and breast cancer, which may be a mechanism by which tumor cells suppress T cell immunity and facilitate tumor progression. Further, VTCN1 may be a useful biomarker for the early detection of ovarian cancer and Renal Cell Carcinoma (RCC).
仅用于科研。不用于诊断过程。未经明确授权不得转售。
蛋白别名: B7 homolog 4; B7-H4; FLJ22418; Immune costimulatory protein B7-H4; Protein B7S1; RP11-229A19.4; T cell costimulatory molecule B7x; V-set domain containing T-cell activation inhibitor 1; vtcn1
基因别名: B7h4; B7s1; B7x; BC032925; Vtcn1
UniProt ID: (Mouse) Q7TSP5
Entrez Gene ID: (Mouse) 242122
分子生物学功能:
immunoglobulin receptor superfamily
