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BAP1 Antibody (PA5-78856) in WB
Western blot analysis of BAP1 in Lane 1: human HeLa whole cell lysate, Lane 2: human HepG2 whole cell lysate, Lane 3: human 22RV1 whole cell lysate, Lane 4: human MCF-7 whole cell lysate, Lane 5: rat thymus tissue lysate, Lane 6: rat kidney tissue lysate, Lane 7: mouse kidney tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was bloc... View More
Product Details
PA5-78856
产品规格
Species Reactivity
Human,
Mouse,
Rat
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
E. coli-derived human BAP1 recombinant protein (Position: M1-E278).
Conjugate
Unconjugated
形式
Lyophilized
浓度
500 µg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 4mg trehalose
内含物
0.05mg sodium azide
保存条件
-20°C
RRID
AB_2745972
Product Specific Information
Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.
Target Information
BRCA1-associated protein-1, or BAP1, interacts with the RING finger domain of BRCA1. The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs), thiol proteases that catalyze proteolytic processing of ubiquitin. In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 and BRCA1 associate in vivo and have overlapping subnuclear localization patterns. BAP1 enhances BRCA1-mediated inhibition of breast cancer cell growth. Northern blot analysis indicates that BAP1 is expressed as a 4-kb mRNA in all human tissues tested, with A 4. 8-kb transcript expressed exclusively in testis. Northern blot analysis and in situ hybridization reveal that BAP1 and BRCA1 are coexpressed during murine breast development and remodeling. The BAP1 gene has been mapped to 3p21. 3, a region of loss of heterozygosity for breast cancer as well as frequently deleted in lung carcinomas. Intragenic homozygous rearrangements and deletions of BAP1 appear in lung carcinoma cell lines. It has been postulated that BAP1 is a tumor suppressor gene that functions in the BRCA1 growth control pathway.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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Bioinformatics
Protein Aliases: Brca1 associated protein 1; BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase); BRCA1-associated protein 1; Cerebral protein 6; cerebral protein-13; DKFZp686N04275; FLJ35406; FLJ37180; Ubiquitin C-terminal hydrolase X4; Ubiquitin carboxyl-terminal hydrolase BAP1; UCH-X4
Gene Aliases: 2300006C11Rik; AA989761; AW553466; BAP1; HUCEP-13; hucep-6; KIAA0272; mKIAA0272; uch-x4; UCHL2
UniProt ID: (Human) Q92560, (Rat) D3ZHS6, (Mouse) Q99PU7
Entrez Gene ID: (Human) 8314, (Rat) 306257, (Mouse) 104416
Molecular Function:
cysteine protease
regulation of cell growth
cellular protein modification process
ubiquitin-dependent protein catabolic process
negative regulation of cell proliferation
protein deubiquitination
monoubiquitinated protein deubiquitination
monoubiquitinated histone H2A deubiquitination
regulation of cell cycle
protein K48-linked deubiquitination
positive regulation of protein targeting to mitochondrion
proteolysis
chromatin modification
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