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          Clicking the images or links will redirect you to a website hosted by BenchSci that provides third-party scientific content. Neither the content nor the BenchSci technology and processes for selection have been evaluated by us; we are providing them as-is and without warranty of any kind, including for use or application of the Thermo Fisher Scientific products presented.

          • Primary Antibodies ›
          • BAP1 Antibodies

          Invitrogen

          BAP1 Polyclonal Antibody

          View all (14) BAP1 antibodies
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          Cite BAP1 Polyclonal Antibody

          Datasheet
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          • Antibody Testing Data (3)
          BAP1 Antibody in Western Blot (WB)
          Group 53 Created with Sketch.

          FIGURE: 1 / 3

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          BAP1 Antibody (PA5-78856) in WB

          Western blot analysis of BAP1 in Lane 1: human HeLa whole cell lysate, Lane 2: human HepG2 whole cell lysate, Lane 3: human 22RV1 whole cell lysate, Lane 4: human MCF-7 whole cell lysate, Lane 5: rat thymus tissue lysate, Lane 6: rat kidney tissue lysate, Lane 7: mouse kidney tissue lysate using 50 µg (reducing conditions) per well. Electrophoresis was performed on 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours and protein was transferred to a nitrocellulose membrane at 150mA for 50-90 minutes. Sample was bloc... View More {{ $ctrl.currentElement.advancedVerification.fullName }} validation info. View more
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          Product Details

          PA5-78856

          Applications
          Tested Dilution
          Publications

          免疫印迹 (WB)

          0.1-0.5 µg/mL
          -
          产品规格

          Species Reactivity

          Human, Mouse, Rat

          Host/Isotype

          Rabbit / IgG

          分类

          Polyclonal

          类型

          Antibody

          Immunogen

          E. coli-derived human BAP1 recombinant protein (Position: M1-E278).
          View immunogen

          Conjugate

          Unconjugated

          形式

          Lyophilized

          浓度

          500 µg/mL

          纯化类型

          Antigen affinity chromatography

          保存液

          PBS with 4mg trehalose

          内含物

          0.05mg sodium azide

          保存条件

          -20°C

          RRID

          AB_2745972

          Product Specific Information

          Reconstitute with 0.2 mL of distilled water to yield a concentration of 500 µg/mL.

          Target Information

          BRCA1-associated protein-1, or BAP1, interacts with the RING finger domain of BRCA1. The N-terminal 240 amino acids of the predicted 729-amino acid human protein show homology to ubiquitin C-terminal hydrolases (UCHs), thiol proteases that catalyze proteolytic processing of ubiquitin. In addition, BAP1 contains an acidic region, a highly charged C-terminal region, and 2 putative nuclear localization signals. BAP1 and BRCA1 associate in vivo and have overlapping subnuclear localization patterns. BAP1 enhances BRCA1-mediated inhibition of breast cancer cell growth. Northern blot analysis indicates that BAP1 is expressed as a 4-kb mRNA in all human tissues tested, with A 4. 8-kb transcript expressed exclusively in testis. Northern blot analysis and in situ hybridization reveal that BAP1 and BRCA1 are coexpressed during murine breast development and remodeling. The BAP1 gene has been mapped to 3p21. 3, a region of loss of heterozygosity for breast cancer as well as frequently deleted in lung carcinomas. Intragenic homozygous rearrangements and deletions of BAP1 appear in lung carcinoma cell lines. It has been postulated that BAP1 is a tumor suppressor gene that functions in the BRCA1 growth control pathway.

          仅用于科研。不用于诊断过程。未经明确授权不得转售。

          References (0)

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          Cite this product

          Bioinformatics

          Protein Aliases: Brca1 associated protein 1; BRCA1 associated protein-1 (ubiquitin carboxy-terminal hydrolase); BRCA1-associated protein 1; Cerebral protein 6; cerebral protein-13; DKFZp686N04275; FLJ35406; FLJ37180; Ubiquitin C-terminal hydrolase X4; Ubiquitin carboxyl-terminal hydrolase BAP1; UCH-X4

          View more View less

          Gene Aliases: 2300006C11Rik; AA989761; AW553466; BAP1; HUCEP-13; hucep-6; KIAA0272; mKIAA0272; uch-x4; UCHL2

          View more View less

          UniProt ID: (Human) Q92560, (Rat) D3ZHS6, (Mouse) Q99PU7

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          Entrez Gene ID: (Human) 8314, (Rat) 306257, (Mouse) 104416

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          Molecular Function: cysteine protease

          Function(s)
          regulation of cell growth cellular protein modification process ubiquitin-dependent protein catabolic process negative regulation of cell proliferation protein deubiquitination monoubiquitinated protein deubiquitination monoubiquitinated histone H2A deubiquitination regulation of cell cycle protein K48-linked deubiquitination positive regulation of protein targeting to mitochondrion proteolysis chromatin modification
          Process(es)
          chromatin binding thiol-dependent ubiquitin-specific protease activity protein binding peptidase activity cysteine-type peptidase activity hydrolase activity
          It has to be done as per old AB suggested Products section.
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          Performance Guarantee

          If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*

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          Get expert recommendations for common problems or connect directly with an on staff expert for technical assistance related to applications, equipment and general product use.

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