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Agrisera
BAR/Phosphinothricin N-acetyltransferase Polyclonal Antibody
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BAR/Phosphinothricin N-acetyltransferase Antibody (AS21-4613) in WB
Samples: From the left: Arabidopsis thaliana wt - 1 Arabidopsis thaliana overexpressing line 1 with BAR (BASTA) - 2 Arabidopsis thaliana overexpressing line 1 with BAR (BASTA) - 320 µg/well of total protein extracted freshly from Arabidopsis thaliana leaves with extraction buffer: 50 m M Tris/Cl p H 7,5, 150 m M Na Cl, 1% Nonidet P 40, 1 tablet Proteinase inhibitor cocktail/ 10 mL. and denatured with SDS sa... View More
产品信息
AS21-4613
产品规格
种属反应
Chemical
宿主/亚型
Rabbit
分类
Polyclonal
类型
Antibody
抗原
KLH-conjugated peptide derived from position 36-50 of Phosphinothricin N-acetyltransferase (BAR or BASTA).
偶联物
Unconjugated
Unconjugated
Unconjugated
形式
Lyophilized
规格
50 µg
纯化类型
Antigen affinity chromatography
保存液
PBS, pH 7.4
内含物
no preservative
保存条件
-20°C, Avoid Freeze/Thaw Cycles
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Store lyophilized/reconstituted at -20°C; once reconstituted make aliquots to avoid repeated freeze-thaw cycles. Please, remember to spin tubes briefly prior to opening them to avoid any losses that might occur from lyophilized material adhering to the cap or sides of the tubes.
For reconstitution add 50 µL of sterile water.
Specific Species Reactivity: BAR (BASTA)
靶标信息
Phosphinothricin N-acetyltransferase (PAT) is a small, soluble bacterial cytosolic enzyme best characterized from Streptomyces species and widely deployed as a selectable marker in plants; when expressed heterologously it accumulates in the compartment where the transgene is targeted (e.g., plastids in transplastomic systems). The enzyme is typically ~183 amino acids (~21 kDa) and adopts a GCN5-related N-acetyltransferase (GNAT) fold that binds acetyl-CoA; structural and biochemical studies support a homodimeric assembly and a conserved active-site architecture for catalysis. Functionally, PAT confers resistance to the glutamine synthetase inhibitor phosphinothricin (glufosinate) by transferring an acetyl group from acetyl-CoA to the free amino group of L-phosphinothricin, producing an inactive N-acetylated derivative that detoxifies the herbicide/antibiotic and prevents downstream ammonia accumulation; substrate profiling indicates strong preference for phosphinothricin over standard proteinogenic amino acids, consistent with its role in self-protection in producing organisms and as an effective resistance determinant in transgenic plants.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: bar; pat
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