Invitrogen

BATF Recombinant Mouse Monoclonal Antibody (MBM7C7)

Name: BATF Recombinant Mouse Monoclonal Antibody (MBM7C7)
Brand: Invitrogen
SKU: 740180M

高级验证

查看其他17个BATF抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (2)
高级验证 (1)

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BATF Antibody (740180M) in Flow

Flow cytometry analysis of BATF Recombinant Mouse Monoclonal Antibody (MBM7C7) (Product # 740180M) was performed using HCT 116 and Ramos cells. Cells were fixed with eBioscience™ Foxp3/Transcription Factor Staining Buffer Set (Product # 00-5523-00), permeabilized and stained intracellularly with 0.25 µg of BATF Recombinant Mouse Monoclonal Antibody (MBM7C7) (Product # 740180M) followed by Goat anti-Mouse IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor™ Plus 488 (Product # A55058, 1:1,000). BATF expression was observed in HCT 116 (pi... View More

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BATF Antibody — Antibody specificity was demonstrated by detection of differential basal expression of the target across cell lines tested owing to their inherent genetic constitution. BATF Recombinant Mouse Monoclonal antibody (MBM7C7) (Product # 740180M) specifically detects BATF expression in HCT 116 cells (pink histogram) while low expression of BATF is seen in Ramos cells (blue histogram). {RE}

产品信息

740180M

应用

建议稀释比

已发表文章

流式细胞分析 (Flow)

0.25 µg/test

产品规格

种属反应

Human

宿主/亚型

Mouse / IgG1, kappa

Expression System

ExpiCHO

分类

Recombinant Monoclonal

类型

Antibody

克隆号

MBM7C7

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

纯化类型

Protein A/G

保存液

PBS, pH 7.4

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

产品详细信息

Recombinant mouse monoclonal antibodies are produced using in vitro expression systems. Recombinant antibodies are produced using specific genes that code for the desired antibodies. These genes are cloned into an expression vector and expressed in vitro. The advantages of recombinant antibodies include better specificity and lot-to-lot consistency.

It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.

靶标信息

BATF is a nuclear basic leucine zipper protein that belongs to the AP-1/ATF superfamily of transcription factors. The leucine zipper of this protein mediates dimerization with members of the Jun family of proteins. This protein is thought to be a negative regulator of AP-1/ATF transcriptional events and blocks cellular transformation by Ras and Fos. BATF also is required for the differentiation of IL-17-producing T helper (TH17) cells, a CD4+ subset of T cells that coordinates the inflammatory response in host defense. In both T cells and B cells, BATF is required for the appropriate regulation of the class-switch recombination.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: activating transcription factor B; B-ATF; B-cell-activating transcription factor; basic leucine zipper transcription factor, ATF-like; Basic leucine zipper transcriptional factor ATF-like; SF-HT-activated gene 2 protein; SFA-2

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基因别名: B-ATF; BATF; BATF1; SFA-2; SFA2

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UniProt ID: (Human) Q16520

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Entrez Gene ID: (Human) 10538

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功能

RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity, RNA polymerase II core promoter proximal region sequence-specific binding transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding transcription factor activity, sequence-specific DNA binding protein binding sequence-specific DNA binding basic leucine zipper transcription factor

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