Invitrogen

BDNF Polyclonal Antibody, PeproTech®

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BDNF Antibody (500-P84-1MG) in WB

Western Blot: To detect Human BDNF by Western Blot analysis BDNF Polyclonal Antibody (Product # 500-P84-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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产品信息

500-P84-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

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酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

中和实验 (Neu)

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免疫染色 (IS)

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Human/Murine/Rat BDNF

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

RRID

AB_2930804

产品详细信息

AA Sequence of recombinant protein: MHSDPARRGE LSVCDSISEW VTAADKKTAV DMSGGTVTVL EKVPVSKGQL KQYFYETKCN PMGYTKEGCR GIDKRHWNSQ CRTTQSYVRA LTMDSKKRIG WRFIRIDTSC VCTLTIKRGR.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Human/Murine/Rat BDNF. Anti-Human/Murine/Rat BDNF-specific antibody was purified by affinity chromatography employing immobilized Human/Murine/Rat BDNF matrix.

Sandwich ELISA: To detect Human/Murine/Rat BDNF by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Human/Murine/Rat BDNF (500-P84Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Human/Murine/Rat BDNF.

Western Blot: To detect Human BDNF by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Human BDNF is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

BDNF (Brain-Derived Neurotrophic Factor) is a member of the neurotrophin family. BDNF is synthesized as pre-proBDNF, followed by cleavage to proBDNF. Although further processing generates the mature, 14 kDa protein, pro-BDNF is biologically active and is secreted in synaptic vesicles along with the mature form. BDNF is widely expressed in the central nervous system and acts in an autocrine and paracrine manner on several classes of neurons. Signaling occurs mainly through the tyrosine kinase receptor TrkB, although binding to the lower-affinity receptor, p75-NTR, has also been demonstrated. BDNF promotes neuronal survival and differentiation and has been shown to play a critical role in memory formation and synaptic regulation. BDNF is induced by cortical neurons, and is necessary for survival of striatal neurons in the brain. Expression of BDNF is reduced in both Alzheimer's and Huntington disease patients, and may also play a role in the regulation of stress response and in the biology of mood disorders.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

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Keratinocytes produce IL-17c to protect peripheral nervous systems during human HSV-2 reactivation.

The Journal of experimental medicine

Peng T,Chanthaphavong RS,Sun S,Trigilio JA,Phasouk K,Jin L,Layton ED,Li AZ,Correnti CE,De van der Schueren W,Vazquez J,O'Day DR,Glass IA,Knipe DM,Wald A,Corey L,Zhu J

Mon Aug 07 00:00:00 EDT 2017

Human endothelial cells secrete neurotropic factors to direct axonal growth of peripheral nerves.

Scientific reports

Grasman JM,Kaplan DL

Published figure using BDNF polyclonal antibody (Product # 500-P84-1MG) in Neutralization

Thu Jun 22 00:00:00 EDT 2017

Mesenchymal stem cells induced to secrete neurotrophic factors attenuate quinolinic acid toxicity: a potential therapy for Huntington's disease.

Experimental neurology

Sadan O,Shemesh N,Barzilay R,Dadon-Nahum M,Blumenfeld-Katzir T,Assaf Y,Yeshurun M,Djaldetti R,Cohen Y,Melamed E,Offen D

Sun Apr 01 00:00:00 EDT 2012

Inhibition of BDNF in multiple myeloma blocks osteoclastogenesis via down-regulated stroma-derived RANKL expression both in vitro and in vivo.

PloS one

Ai LS,Sun CY,Zhang L,Zhou SC,Chu ZB,Qin Y,Wang YD,Zeng W,Yan H,Guo T,Chen L,Yang D,Hu Y

Wed Mar 20 00:00:00 EDT 2013

Inhibition of BDNF in multiple myeloma blocks osteoclastogenesis via down-regulated stroma-derived RANKL expression both in vitro and in vivo.

PloS one

Ai LS,Sun CY,Zhang L,Zhou SC,Chu ZB,Qin Y,Wang YD,Zeng W,Yan H,Guo T,Chen L,Yang D,Hu Y

Wed Mar 20 00:00:00 EDT 2013

Transcellular induction of neuropeptide Y expression by NT4 and BDNF.

Proceedings of the National Academy of Sciences of the United States of America

Wirth MJ,Patz S,Wahle P

Tue Feb 22 00:00:00 EST 2005

The neurotrophin-trk receptor axes are critical for the growth and progression of human prostatic carcinoma and pancreatic ductal adenocarcinoma xenografts in nude mice.

Clinical cancer research : an official journal of the American Association for Cancer Research

Miknyoczki SJ,Wan W,Chang H,Dobrzanski P,Ruggeri BA,Dionne CA,Buchkovich K

Sat Jun 01 00:00:00 EDT 2002

生物信息学

蛋白别名: Abrineurin; anorexia BDNF; BDNF; brain derived neurothrophic factor; brain derived neurotrophic factor; Brain-derived neurotrophic factor; H-BDNF; neurotrophin

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基因别名: ANON2; BDNF; BULN2

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UniProt ID: (Human) P23560, (Rat) P23363, (Mouse) P21237

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Entrez Gene ID: (Human) 627, (Rat) 24225, (Mouse) 12064

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功能

neurotrophin TRKB receptor binding growth factor activity receptor binding protein binding neurotrophic factor

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