Invitrogen

BNIP3 Recombinant Polyclonal Antibody (8HCLC)

高级验证

This Antibody was verified by Cell treatment to ensure that the antibody binds to the antigen stated.

查看其他15个BNIP3抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (2)

BNIP3 Antibody in Immunocytochemistry (ICC/IF)

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BNIP3 Antibody (710728) in ICC/IF

For immunofluorescence analysis, cobalt chloride (150 uM, 48h) treated HeLa cells were fixed with methanol for detection of BNIP3 using Anti-BNIP3 Recombinant Rabbit Polyclonal Antibody (Product # 710728, 2 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Cytoskeleton was stained with alpha-tubulin Monoclonal Antibody (Product # 32-2500, 1 µg/mL) followed by Goat anti-Mouse IgG Secondary Antibody, Alexa Fluor® 594 conjugate (Product # A-11032, ... View More

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Altered expression of proteins upon cell treatment demonstrates antibody specificity. Immunofluorescence analysis of BNIP3 using BNIP3 Antibody (8HCLC) Recombinant Rabbit Polyclonal (Product # 710728) shows expression of BNIP3 in the cytoplasm of HeLa cells upon treatment with cobalt chloride. {TM}

产品信息

710728

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1- 2 µg/mL

免疫细胞化学 (ICC/IF)

2 µg/mL

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

Expression System

Expi293

分类

Recombinant Polyclonal

类型

Antibody

克隆号

8HCLC

抗原

Protein corresponding to Human BNIP3 (aa 1-140)

查看免疫原

偶联物

Unconjugated

形式

Liquid

浓度

0.5 mg/mL

纯化类型

Protein A

保存液

PBS, pH 7.2

内含物

0.09% sodium azide

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Wet ice

RRID

AB_2607982

产品详细信息

This antibody is predicted to react with Monkey, Bovine and Equine.

Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.

靶标信息

NIP3 is a member of the BCL2/adenovirus E1B 19 kd-interacting protein (BNIP) family. It interacts with the E1B 19 kDa protein which is responsible for the protection of virally-induced cell death, as well as E1B 19 kDa-like sequences of BCL2, also an apoptotic protector. NIP3 contains a BH3 domain and a transmembrane domain, which have been associated with pro-apoptotic function. The dimeric mitochondrial protein is known to induce apoptosis, even in the presence of BCL2.

仅用于科研。不用于诊断过程。未经明确授权不得转售。