Invitrogen

BRD7 Polyclonal Antibody

高级验证

This Antibody was verified by Knockout to ensure that the antibody binds to the antigen stated.

查看其他14个BRD7抗体

说明书

实验方案

技术支持

说明书

实验方案

技术支持

抗体检测数据 (2)
高级验证 (1)

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BRD7 Antibody (PA5-96105) in WB

Western blot analysis of BRD7 in extracts of various cell lines. Samples were incubated with BRD7 Polyclonal antibody (Product # PA5-96105) using a dilution of 1:1,000, followed by HRP Goat Anti-Rabbit IgG (H+L) at a dilution of 1:10,000. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

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BRD7 Antibody — Antibody specificity was demonstrated by knockout of the target protein. Loss of signal was observed for target protein in BRD7 knockout (KO) 293T cell line using Anti-BRD7 Polyclonal Antibody (Product # PA5-96105). {KO}

产品信息

PA5-96105

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

产品规格

种属反应

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Recombinant fusion protein containing a sequence corresponding to amino acids 1-95 of human BRD7 (NP_0373952)

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.81 mg/mL

纯化类型

Affinity Chromatography

保存液

PBS, pH 7.3, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20° C, Avoid Freeze/Thaw Cycles

运输条件

Wet ice

RRID

AB_2807907

产品详细信息

Immunogen sequence: MGKKHKKHKS DKHLYEEYVE KPLKLVLKVG GNEVTELSTG SSGHDSSLFE DKNDHDKHKD RKRKKRKKGE KQIPGEEKGR KRRRVKEDKK KRDRD; Positive Samples: U-87MG, HepG2, SW620; Cellular Location: Nucleus

靶标信息

BRD7 acts both as coactivator and as corepressor. For example, it activates the Wnt signaling pathway in a DVL1-dependent manner by negatively regulating the GSK3B phosphotransferase activity. In addition, it is required for TP53-mediated cell-cycle arrest in response to oncogene activation. BRD7 promotes acetylation of TP53 at Lys-382, and thereby promotes efficient recruitment of TP53 to target promoters, inhibiting cell cycle progression from G1 to S phase.

仅用于科研。不用于诊断过程。未经明确授权不得转售。