Invitrogen

BRG1 Polyclonal Antibody

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BRG1 Antibody in Western Blot (WB)

BRG1 Antibody in Western Blot (WB)

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BRG1 Antibody (PA5-17008) in WB

Western blot analysis of Brg1 in extracts from MOLT4 and OVCAR3 cell lines using Brg1 polyclonal antibody (Product # PA5-17008).

BRG1 Antibody in Western Blot (WB)

BRG1 Antibody in Western Blot (WB)

BRG1 Antibody in Western Blot (WB)

BRG1 Antibody in Western Blot (WB)

Product Image Gallery

BRG1 Antibody in Western Blot (WB) — Western blot analysis of Brg1 in extracts from MOLT4 and OVCAR3 cell lines using Brg1 polyclonal antibody (Product # PA5-17008).

BRG1 Antibody in Western Blot (WB) — Fig 3 Subcellular localization of USP46 complexes in LCLs. Wild type LCLs were extracted into five subcellular fractions [cytoplasm (C), membrane (M), soluble nuclear (Nu), chromatin (Ch), cytoskeleton (CS)], resolved by SDS-PAGE and probed for EBNA3 proteins, RBPJ, and components of the USP46 complex (USP46, WDR20, and WDR48). Fraction purity was assessed by probing for tubulin, BRG1, Histone H2B, and LaminB.

BRG1 Antibody in Western Blot (WB) — Published figure using BRG1 polyclonal antibody (Product # PA5-17008) in Western Blot

BRG1 Antibody in Western Blot (WB) — Published figure using BRG1 polyclonal antibody (Product # PA5-17008) in Western Blot

产品信息

PA5-17008

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Synthetic peptide corresponding to amino acids surrounding Pro680 of human Brg1

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

31 µg/mL

纯化类型

Antigen affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

no preservative

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_10982978

产品详细信息

It is not recommended to aliquot this antibody.

靶标信息

Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

The EBNA3 family of Epstein-Barr virus nuclear proteins associates with the USP46/USP12 deubiquitination complexes to regulate lymphoblastoid cell line growth.

PLoS pathogens

Ohashi M,Holthaus AM,Calderwood MA,Lai CY,Krastins B,Sarracino D,Johannsen E

Published figure using BRG1 polyclonal antibody (Product # PA5-17008) in Western Blot

Wed Apr 01 00:00:00 EDT 2015

生物信息学

蛋白别名: ATP-dependent helicase SMARCA4; BAF190A; brahma protein-like 1; BRG1-associated factor 190A; BRM/SWI2-related gene 1; FLJ39786; global transcription activator homologous sequence; homeotic gene regulator; Mitotic growth and transcription activator; nuclear protein GRB1; Protein brahma homolog 1; Protein BRG-1; SNF2-beta; SNF2-like 4; sucrose nonfermenting-like 4; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; Transcription activator BRG1

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基因别名: b2b508.1Clo; b2b692Clo; BAF190; BAF190A; brg-1; BRG1; CSS4; HP1-BP72; hSNF2b; MRD16; RTPS2; SMARCA4; SNF2; SNF2B; SNF2beta; SNF2L4; SNF2LB; SW1/SNF; SWI2

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UniProt ID: (Human) P51532, (Mouse) Q3TKT4

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Entrez Gene ID: (Human) 6597, (Mouse) 20586, (Rat) 171379

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RNA polymerase II core promoter proximal region sequence-specific DNA binding RNA polymerase II distal enhancer sequence-specific DNA binding RNA polymerase II transcription coactivator activity RNA polymerase I CORE element sequence-specific DNA binding p53 binding transcription coactivator activity transcription corepressor activity helicase activity protein binding ATP binding DNA-dependent ATPase activity transcription factor binding Tat protein binding nucleosomal DNA binding protein N-terminus binding androgen receptor binding DNA polymerase binding lysine-acetylated histone binding nucleotide binding RNA polymerase II regulatory region sequence-specific DNA binding DNA binding chromatin binding hydrolase activity hydrolase activity, acting on acid anhydrides hydrolase activity, acting on acid anhydrides, in phosphorus-containing anhydrides ATPase activity protein complex binding histone binding DNA helicase

参与通路

negative regulation of transcription from RNA polymerase II promoter neural retina development nucleosome disassembly chromatin remodeling transcription, DNA-templated regulation of transcription from RNA polymerase II promoter negative regulation of transcription from RNA polymerase II promoter during mitosis spermatid development nervous system development covalent chromatin modification positive regulation of Wnt signaling pathway negative regulation of cell growth ATP-dependent chromatin remodeling positive regulation by host of viral transcription negative regulation of transcription, DNA-templated positive regulation of transcription, DNA-templated positive regulation of transcription from RNA polymerase II promoter positive regulation of sequence-specific DNA binding transcription factor activity negative regulation of androgen receptor signaling pathway positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter positive regulation of glucose mediated signaling pathway positive regulation of pri-miRNA transcription from RNA polymerase II promoter beta-catenin-TCF complex assembly negative regulation of G1/S transition of mitotic cell cycle cell morphogenesis blood vessel development vasculogenesis in utero embryonic development blastocyst growth blastocyst hatching liver development outflow tract morphogenesis nucleosome assembly methylation-dependent chromatin silencing regulation of transcription, DNA-templated transcription from RNA polymerase II promoter glial cell fate determination heart development positive regulation of cell proliferation DNA methylation on cytosine within a CG sequence chromatin modification stem cell population maintenance neurogenesis extracellular matrix organization keratinocyte differentiation regulation of cell migration forebrain development hindbrain development embryonic hindlimb morphogenesis aortic smooth muscle cell differentiation negative regulation of apoptotic process positive regulation of DNA binding histone H3 acetylation positive regulation of cell differentiation embryonic organ morphogenesis epidermis morphogenesis definitive erythrocyte differentiation heart trabecula formation pharyngeal arch artery morphogenesis lens fiber cell development