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BRG1 Antibody (710894) in ICC/IF
For immunofluorescence analysis, MDA-MB-231 cells were fixed and permeabilized for detection of endogenous BRG1 using Anti- BRG1 Recombinant Rabbit Polyclonal Antibody (Product # 710894, 5 µg/mL) and labeled with Goat anti-Rabbit IgG (Heavy Chain) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034, 1:2000). Panel a) shows representative cells that were stained for detection and localization of BRG1 protein (green), Panel b) is stained for nuclei (blue) using SlowFade® Gold Antifade Mountant with DAPI (Product # S... View More
产品信息
710894
产品规格
种属反应
Human
宿主/亚型
Rabbit
/ IgG
Expression System
Expi293
分类
Recombinant Superclonal
类型
Antibody
克隆号
16 HCLC
抗原
Protein corresponding to human Brg1 (aa1429-aa1641)
偶联物
形式
Liquid
浓度
0.5 mg/mL
纯化类型
Protein A
保存液
PBS, pH 7.4
内含物
0.09% sodium azide
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Wet ice
RRID
AB_2716897
产品详细信息
This antibody is predicted to react with Monkey, Horse, Cat, Dog
Recombinant rabbit polyclonal antibodies are unique offerings from Thermo Fisher Scientific. They are comprised of a selection of multiple different recombinant monoclonal antibodies, providing the best of both worlds - the sensitivity of polyclonal antibodies with the specificity of monoclonal antibodies - all delivered with the consistency only found in a recombinant antibody. While functionally the same as a polyclonal antibody - recognizing multiple epitope sites on the target and producing higher detection sensitivity for low abundance targets - a recombinant rabbit polyclonal antibody has a known mixture of light and heavy chains. The exact population can be produced in every lot, circumventing the biological variability typically associated with polyclonal antibody production.
靶标信息
Brg1 (Brahma-related gene 1) is an ATPase subunit of SWI2/SNF2-like chromatin-remodeling complexes that enable access of regulatory and effector proteins in transcription, DNA repair and DNA replication. Although Brg1-containing complexes are not essential for general cell survival, they participate in transcriptional regulation of several hundred genes including those involved in interferon and stress response, immune cells differentiation, neurogenesis, cell cycle etc. and is absolutely necessary for mouse embryogenesis. Brg1 is also involved in cell growth arrest, senescence and tumour supression.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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生物信息学
蛋白别名: ATP-dependent helicase SMARCA4; BAF190A; brahma protein-like 1; BRG1-associated factor 190A; BRM/SWI2-related gene 1; FLJ39786; global transcription activator homologous sequence; homeotic gene regulator; Mitotic growth and transcription activator; nuclear protein GRB1; Protein brahma homolog 1; Protein BRG-1; SNF2-beta; SNF2-like 4; sucrose nonfermenting-like 4; SWI/SNF-related matrix-associated actin-dependent regulator of chromatin subfamily A member 4; Transcription activator BRG1
基因别名: BAF190; BAF190A; BRG1; CSS4; hSNF2b; MRD16; RTPS2; SMARCA4; SNF2; SNF2B; SNF2L4; SNF2LB; SWI2
UniProt ID: (Human) P51532
Entrez Gene ID: (Human) 6597
RNA polymerase II core promoter proximal region sequence-specific DNA binding
RNA polymerase II distal enhancer sequence-specific DNA binding
RNA polymerase II transcription coactivator activity
RNA polymerase I CORE element sequence-specific DNA binding
p53 binding
transcription coactivator activity
transcription corepressor activity
helicase activity
protein binding
ATP binding
DNA-dependent ATPase activity
transcription factor binding
Tat protein binding
nucleosomal DNA binding
protein N-terminus binding
androgen receptor binding
DNA polymerase binding
lysine-acetylated histone binding
DNA helicase
negative regulation of transcription from RNA polymerase II promoter
neural retina development
nucleosome disassembly
chromatin remodeling
transcription, DNA-templated
regulation of transcription from RNA polymerase II promoter
negative regulation of transcription from RNA polymerase II promoter during mitosis
spermatid development
nervous system development
covalent chromatin modification
positive regulation of Wnt signaling pathway
negative regulation of cell growth
ATP-dependent chromatin remodeling
positive regulation by host of viral transcription
negative regulation of transcription, DNA-templated
positive regulation of transcription, DNA-templated
positive regulation of transcription from RNA polymerase II promoter
positive regulation of sequence-specific DNA binding transcription factor activity
negative regulation of androgen receptor signaling pathway
positive regulation of transcription of nuclear large rRNA transcript from RNA polymerase I promoter
positive regulation of glucose mediated signaling pathway
positive regulation of pri-miRNA transcription from RNA polymerase II promoter
beta-catenin-TCF complex assembly
negative regulation of G1/S transition of mitotic cell cycle
