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Nordic
Bacillus thermoproteolyticus rokko Thermolysin Polyclonal Antibody
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产品信息
NE153-PAB
产品规格
种属反应
Bacteria
宿主/亚型
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
抗原
Bacillus thermoproteolyticus rokko Thermolysin
偶联物
形式
Lyophilized
浓度
2 mg/mL
纯化类型
Antigen affinity chromatography
保存液
PBS with 0.5% dextran
内含物
no preservative
保存条件
Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
运输条件
Ambient (domestic); Wet ice (international)
产品详细信息
Reconstituted by adding 0.5 mL sterile distilled water, spun down to remove insoluble particles, divided into small aliquots, frozen and stored at or below -20°C.
Cross-reactivities against enzymes of other sources may occur but have not been determined.
Prior to use, an aliquot is thawed slowly at ambient temperature, spun down again and used to prepare working dilutions by adding sterile phosphate buffered saline (PBS, pH 7.2). Repeated thawing and freezing should be avoided. Working dilutions should be stored at 4°C, not refrozen, and preferably used t he same day. If a slight precipitation occurs upon storage, this should be removed by centrifugation. It will not affect the performance of the product.
靶标信息
Thermolysin is a thermostable extracellular zinc metalloproteinase used to cleave peptide bonds at specific junctions. It requires one zinc ion for enzyme activity and four calcium ions for structural stability. Thermolysin preferentially cleaves at the N-terminus of the hydrophobic residues leucine, phenylalanine, valine, isoleucine, alanine and methionine. The optimal digestion temperature range is 65-85 C.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
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