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Invitrogen
| 应用 | 建议稀释比 | 已发表文章 |
|---|---|---|
免疫印迹 (WB) |
- | 查看1篇已发表文章 1篇已发表文章 |
流式细胞分析 (Flow) |
0.125 µg/test | - |
| 产品规格 | |
|---|---|
种属反应 |
Virus |
已发表种属 |
Virus |
宿主/亚型 |
Mouse / IgG2a |
推荐的同型对照 |
Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience™ |
分类 |
Monoclonal |
类型 |
Antibody |
克隆号 |
AcV1 |
偶联物 |
PE
|
激发/发射光谱 |
查看光谱
|
形式 |
Liquid |
浓度 |
0.2 mg/mL |
纯化类型 |
Affinity chromatography |
保存液 |
PBS, pH 7.2 |
内含物 |
0.09% sodium azide |
保存条件 |
4° C, store in dark, DO NOT FREEZE! |
RRID |
AB_1633369 |
Description: The AcV1 monoclonal antibody reacts with the gp64 envelope protein of the baculovirus Autographa californica (AcMNPV). The gp64 envelope protein is essential for virus infectivity and is expressed on the surface of baculovirus-infected cells within six hours of infection. The AcV1 antibody can be used in flow cytometry-based viral titration experiments.
Applications Reported: This AcV1 antibody has been reported for use in flow cytometric analysis. This antibody can be used in identifying virally-infected insect cells and determining viral titers.
Applications Tested: This AcV1 antibody has been tested by flow cytometric analysis of baculovirus-infected insect cells. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.
Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.
Filtration: 0.2 µm post-manufacturing filtered.
Baculoviruses are known to infect invertebrates, with over 600 species having been described. Immature (larval) forms of moth species are the most common hosts, but these viruses have also been found infecting sawflies, mosquitoes, and shrimp. Although baculoviruses are capable of entering mammalian cells in culture, they are not known to be capable of replication in mammalian or other vertebrate animal cells. The gp64 envelope protein is essential for virus infectivity and is expressed on the surface of baculovirus-infected cells within six hours of infection.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
Watch the video to learn how to use the Invitrogen Flow Cytometry Panel Builder to build your next flow cytometry panel in 5 easy steps.
蛋白别名: BV gp64
