Invitrogen

Bcl-2 Polyclonal Antibody

Bcl-2 Antibody in Immunocytochemistry (ICC/IF)

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Bcl-2 Antibody (PA5-27094) in ICC/IF

Immunocytochemistry-Immunofluorescence analysis of Bcl-2 was performed in THP-1 cells fixed in 4% paraformaldehyde at RT for 15 min. Green: Bcl-2 Polyclonal Antibody (Product # PA5-27094) diluted at 1:500. Blue: Fluoroshield with DAPI.

Bcl-2 Antibody in Immunohistochemistry (Paraffin) (IHC (P))

Bcl-2 Antibody in Immunohistochemistry (IHC)

Product Image Gallery

Bcl-2 Antibody — Figure 3. Combined treatment downregulates the expression of anti-apoptotic proteins and enhances the expression of pro-apoptotic proteins. Western blot analysis results of (A) anti-apoptotic and (B) pro-apoptotic protein expression 24 h following cis, bor and cis plus bortezomib combination treatment. C, control; cis, cisplatin; bor, bortezomib; Bcl-2, B-cell lymphoma-2; Bcl-xL, B-cell lyphoma-extra large; Bim, B-cell lyphoma-2-like 11; Bik, Bcl-2-interacting killer. {TM}

产品信息

PA5-27094

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:3,000

查看16篇已发表文章 16篇已发表文章

免疫组化 (IHC)

查看5篇已发表文章 5篇已发表文章

免疫组化（石蜡） (IHC (P))

1:100-1:1,000

查看2篇已发表文章 2篇已发表文章

免疫细胞化学 (ICC/IF)

1:100-1:1,000

产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

Carrier-protein conjugated synthetic peptide encompassing a sequence within the N-terminus region of human Bcl-2. The exact sequence is proprietary.

查看免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

0.2 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS, pH 7, with 1% BSA, 20% glycerol

内含物

0.025% ProClin 300

保存条件

Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.

运输条件

Ambient (domestic); Wet ice (international)

AB_2544570

产品详细信息

Recommended positive controls: THP-1, HL-60, mouse spleen, PA-1, OKT3.

Predicted reactivity: Mouse (100%), Rat (100%), Dog (100%), Cat (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

BCL-2 is a key regulator of apoptosis that functions to either inhibit or promote cell death. The BCL-2 family members are also characterized by dimerizing to further modulate apoptosis. Bag1, for example, has been found to form a heterodimer with BCL-2 resulting in the enhancement of the anti-apoptotic effect of BCL-2. Bax and Bak have been shown to play a critical role in cytochrome c release from mitochondria and thus initiate apoptosis. Bax exerts a pro-apoptotic rather than an anti-apoptotic effect on cells. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma. In most follicular lymphomas, neoplastic germinal centers express high levels of BCL-2 alpha protein, whereas the normal or hyperplastic germinal centers are negative. Two transcript variants of BCL-2, produced by alternate splicing, differ in their C-terminal ends. The overexpression of BCL-2 has been linked to human cancers such as B-cell lymphoma and prostate cancer.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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PA5-27094 was used in Immunocytochemistry, Immunohistochemistry (Paraffin), Western Blot to draw attention to the favorable cognitive effects of memantine/aripiprazole combination in autistic subjects which could be mediated via enhancing CREB/BDNF signaling with increased expression of astrocytic Glt-1 and restoration of GABA/glutamate balance, leading to inhibition of hippocampal NFTs formation and neuronal apoptosis.

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Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Western Blot

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Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Western Blot

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Published figure using Bcl-2 polyclonal antibody (Product # PA5-27094) in Western Blot

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生物信息学

蛋白别名: Apoptosis regulator Bcl-2; B cell lymphoma 2 associated oncogene; B-cell CLL/lymphoma 2; B-cell leukemia/lymphoma 2; Bcl 2; Bcl2-like protein; OTTHUMP00000163680; protein phosphatase 1, regulatory subunit 50

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基因别名: AW986256; Bcl-2; BCL2; C430015F12Rik; D630044D05Rik; D830018M01Rik; PPP1R50

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UniProt ID: (Human) P10415, (Rat) P49950, (Mouse) P10417

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Entrez Gene ID: (Human) 596, (Rat) 24224, (Mouse) 12043

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