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Invitrogen
Bcl-xL Polyclonal Antibody
Advanced Verification
This Antibody was verified by Knockdown to ensure that the antibody binds to the antigen stated.
FIGURE: 1 / 5
Bcl-xL Antibody (PA5-17805) in WB
Western blot was performed using Anti-Bcl-xL Polyclonal Antibody(Product # PA5-17805) and a 25kDa band corresponding to Bcl-xL was observed across cell lines tested. Whole cell extracts (30 µg lysate) of A-431 (Lane 1), NIH:OVCAR-3 (Lane 2), Hep G2 (Lane 3), NIH/3T3 (Lane 4), PC-12 (Lane 5), A549 (Lane 6) were electrophoresed using NuPAGE™ 4-12% Bis-Tris Protein Gel (Product # NP0322BOX). Resolved proteins were then transferred onto a Nitrocellulose membrane (Product # IB23001) by iBlot® 2 Dry Blotting System (Product # IB21001). The blot ... View More
Product Details
PA5-17805
Applications
Tested Dilution
Publications
产品规格
Species Reactivity
Human,
Mouse,
Non-human primate,
Rat
Published species
Rat
Host/Isotype
Rabbit
/ IgG
分类
Polyclonal
类型
Antibody
Immunogen
Synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL
Conjugate
Unconjugated
形式
Liquid
浓度
82.8 µg/mL
纯化类型
Antigen affinity chromatography
保存液
0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol
内含物
no preservative
保存条件
-20°C
RRID
AB_10977568
Product Specific Information
It is not recommended to aliquot this antibody.
This antibody is not cross-reactive with other Bcl-2 family members.
Target Information
BCL-XL is a part of the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BCL-XL is located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential and controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.
仅用于科研。不用于诊断过程。未经明确授权不得转售。
Bioinformatics
Protein Aliases: anti-apoptosis regulatory protein; Apoptosis regulator Bcl-X; B cell lymphoma 2 like; B cell lymphoma like X; B-cell leukemia/lymphoma x; Bcl-2-like protein 1; bcl-xS; Bcl2-L-1; BCL2-like 1; BCLXL; BCLXS; DKFZp781P2092; OTTHUMP00000030550; OTTHUMP00000030552; OTTHUMP00000030555; OTTHUMP00000030556; OTTHUMP00000030557; protein phosphatase 1, regulatory subunit 52; RP5-857M17.3
Gene Aliases: Bcl(X)L; Bcl-X; Bcl-XL; BCL-XL/S; bcl2-L-1; BCL2L; BCL2L1; BCLX; Blc2l; PPP1R52
UniProt ID: (Human) Q07817, (Mouse) Q64373, (Rat) P53563
Entrez Gene ID: (Human) 598, (Mouse) 12048, (Rat) 24888
cytokinesis
ovarian follicle development
in utero embryonic development
release of cytochrome c from mitochondria
endocytosis
mitotic cell cycle checkpoint
germ cell development
spermatogenesis
cell proliferation
positive regulation of cell proliferation
male gonad development
intrinsic apoptotic signaling pathway in response to DNA damage
apoptotic mitochondrial changes
fertilization
suppression by virus of host apoptotic process
response to cytokine
growth
negative regulation of apoptotic process
negative regulation of neuron apoptotic process
response to cycloheximide
regulation of mitochondrial membrane permeability
neuron apoptotic process
regulation of mitochondrial membrane potential
cellular process regulating host cell cycle in response to virus
mitochondrion morphogenesis
cellular response to amino acid stimulus
cellular response to alkaloid
cellular response to gamma radiation
apoptotic process in bone marrow
negative regulation of establishment of protein localization to plasma membrane
negative regulation of release of cytochrome c from mitochondria
extrinsic apoptotic signaling pathway in absence of ligand
hepatocyte apoptotic process
negative regulation of execution phase of apoptosis
negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage
negative regulation of extrinsic apoptotic signaling pathway in absence of ligand
negative regulation of intrinsic apoptotic signaling pathway
positive regulation of intrinsic apoptotic signaling pathway
response to ischemia
apoptotic process
response to radiation
response to virus
regulation of apoptotic process
positive regulation of apoptotic process
negative regulation of cysteine-type endopeptidase activity involved in apoptotic process
negative regulation of anoikis
response to hypoxia
response to oxidative stress
response to inorganic substance
response to organonitrogen compound
response to lead ion
response to organic cyclic compound
response to hydrogen peroxide
response to peptide hormone
response to electrical stimulus
Performance Guarantee
If an Invitrogen™ antibody doesn't perform as described on our website or datasheet,we'll replace the product at no cost to you, or provide you with a credit for a future purchase.*
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