Invitrogen

Bcl-xL Monoclonal Antibody (C.85.1)

Bcl-xL Antibody in Immunohistochemistry (Paraffin) (IHC (P))

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Bcl-xL Antibody (MA5-15142) in IHC (P)

Immunohistochemical analysis of Bcl-xL was performed using formalin-fixed paraffin-embedded human colon adenocarcinoma tissue sections. To expose the target protein, heat-induced epitope retrieval was performed on de-paraffinized sections using eBioscience™ IHC Antigen Retrieval Solution - High pH (10X) (Product # 00-4956-58) diluted to 1X solution in water in a decloaking chamber at 110 degree Celsius for ... View More

Bcl-xL Antibody in Immunoprecipitation (IP)

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产品信息

MA5-15142

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:1,000

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免疫组化（石蜡） (IHC (P))

1:600-1:2400

免疫细胞化学 (ICC/IF)

1:200-1:800

流式细胞分析 (Flow)

1:200-1:800

免疫沉淀 (IP)

1:100

产品规格

种属反应

Human, Mouse, Non-human primate, Rat

已发表种属

Human, Mouse

宿主/亚型

Rabbit / IgG

分类

Monoclonal

类型

Antibody

克隆号

C.85.1

抗原

Synthetic peptide corresponding to residues surrounding Asp61 of human Bcl-xL

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

144 µg/mL

纯化类型

Affinity chromatography

保存液

0.01M HEPES, pH 7.5, with 0.15M NaCl, 100µg/mL BSA, 50% glycerol

内含物

<0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_10979212

产品详细信息

It is not recommended to aliquot this antibody.

This antibody is not cross-reactive with other Bcl-2 family members.

靶标信息

BCL-XL is a part of the BCL-2 protein family. BCL-2 family members form hetero- or homodimers and act as anti- or pro-apoptotic regulators that are involved in a wide variety of cellular activities. BCL-XL is located at the outer mitochondrial membrane, and have been shown to regulate outer mitochondrial membrane channel (VDAC) opening. VDAC regulates mitochondrial membrane potential and controls the production of reactive oxygen species and release of cytochrome C by mitochondria, both of which are the potent inducers of cell apoptosis. Two alternatively spliced transcript variants, which encode distinct isoforms, have been reported. The longer isoform acts as an apoptotic inhibitor and the shorter form acts as an apoptotic activator.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Systematic discovery of mutation-directed neo-protein-protein interactions in cancer.

Cell

Mo X,Niu Q,Ivanov AA,Tsang YH,Tang C,Shu C,Li Q,Qian K,Wahafu A,Doyle SP,Cicka D,Yang X,Fan D,Reyna MA,Cooper LAD,Moreno CS,Zhou W,Owonikoko TK,Lonial S,Khuri FR,Du Y,Ramalingam SS,Mills GB,Fu H

MA5-15142 was used in Western blotting following immunoprecipitation to identify prevalent variant-enabled neomorph-protein-protein interactions (neoPPI) with a quantitative high-throughput differential screening (qHT-dS) platform.

Thu May 26 00:00:00 UTC 2022

Morphological Adaptation in the Jejunal Mucosa after Iso-Caloric High-Fat versus High-Carbohydrate Diets in Healthy Volunteers: Data from a Randomized Crossover Study.

Nutrients

Casselbrant A,Wallenius V,Elebring E,Marschall HU,Johansson BR,Helander HF,Fändriks L

MA5-15142 was used in Western Blotting to support the notion that mitochondrial HMGCS2 is regulated by the fat content of the diet and is involved in the expression of monosaccharide transporters.

Tue Oct 04 00:00:00 UTC 2022

Acetone Extract of Cornus officinalis Leaves Exerts Anti-Melanoma Effects via Inhibiting STAT3 Signaling.

OncoTargets and therapy

Xu R,Zeng M,Wu Y,Wang S,Zhang B,Zhang J,Kan Y,Li B,Cao B,Zheng X,Feng W

Published figure using Bcl-xL monoclonal antibody (Product # MA5-15142) in Western Blot

Sat Apr 23 00:00:00 UTC 2022

Acetone Extract of Cornus officinalis Leaves Exerts Anti-Melanoma Effects via Inhibiting STAT3 Signaling.

OncoTargets and therapy

Xu R,Zeng M,Wu Y,Wang S,Zhang B,Zhang J,Kan Y,Li B,Cao B,Zheng X,Feng W

Published figure using Bcl-xL monoclonal antibody (Product # MA5-15142) in Western Blot

Sat Apr 23 00:00:00 UTC 2022

Protective Effect of a Fucose-Rich Fucoidan Isolated from Saccharina japonica against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in Zebrafish.

Marine drugs

Su W,Wang L,Fu X,Ni L,Duan D,Xu J,Gao X

Published figure using Bcl-xL monoclonal antibody (Product # MA5-15142) in Western Blot

Mon Jun 15 00:00:00 UTC 2020

Protective Effect of a Fucose-Rich Fucoidan Isolated from Saccharina japonica against Ultraviolet B-Induced Photodamage In Vitro in Human Keratinocytes and In Vivo in Zebrafish.

Marine drugs

Su W,Wang L,Fu X,Ni L,Duan D,Xu J,Gao X

Published figure using Bcl-xL monoclonal antibody (Product # MA5-15142) in Western Blot

Mon Jun 15 00:00:00 UTC 2020

MicroRNA-325-3p Facilitates Immune Escape of Mycobacterium tuberculosis through Targeting LNX1 via NEK6 Accumulation to Promote Anti-Apoptotic STAT3 Signaling.

mBio

Fu B,Xue W,Zhang H,Zhang R,Feldman K,Zhao Q,Zhang S,Shi L,Pavani KC,Nian W,Lin X,Wu H

MA5-15142 was used in Western Blotting to contribution to the understanding of the immune escape of M. tuberculosis, and they provide a theoretical basis for the development of therapeutic approaches for drug-resistant TB.

Tue Jun 02 00:00:00 UTC 2020

ALA-mediated biphasic downregulation of α-7nAchR/HIF-1α along with mitochondrial stress modulation strategy in mammary gland chemoprevention.

Journal of cellular physiology

Roy S,Singh M,Sammi SR,Pandey R,Kaithwas G

Published figure using Bcl-xL monoclonal antibody (Product # MA5-15142) in Western Blot

Mon Apr 01 00:00:00 UTC 2019

Activation of prolyl hydroxylase-2 for stabilization of mitochondrial stress along with simultaneous downregulation of HIF-1α/FASN in ER + breast cancer subtype.

Cell biochemistry and function

Devi U,Singh M,Roy S,Gupta PS,Ansari MN,Saeedan AS,Kaithwas G

Published figure using Bcl-xL monoclonal antibody (Product # MA5-15142) in Western Blot

Sat Jun 01 00:00:00 UTC 2019

Alpha-linolenic acid stabilizes HIF-1 α and downregulates FASN to promote mitochondrial apoptosis for mammary gland chemoprevention.

Oncotarget

Roy S,Rawat AK,Sammi SR,Devi U,Singh M,Gautam S,Yadav RK,Rawat JK,Singh L,Ansari MN,Saeedan AS,Pandey R,Kumar D,Kaithwas G

Published figure using Bcl-xL monoclonal antibody (Product # MA5-15142) in Western Blot

Tue Sep 19 00:00:00 UTC 2017

生物信息学

蛋白别名: anti-apoptosis regulatory protein; Apoptosis regulator Bcl-X; B cell lymphoma 2 like; B cell lymphoma like X; B-cell leukemia/lymphoma x; Bcl-2-like protein 1; bcl-xS; Bcl2-L-1; BCL2-like 1; BCLXL; BCLXS; DKFZp781P2092; OTTHUMP00000030550; OTTHUMP00000030552; OTTHUMP00000030555; OTTHUMP00000030556; OTTHUMP00000030557; protein phosphatase 1, regulatory subunit 52; RP5-857M17.3

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基因别名: Bcl(X)L; Bcl-X; Bcl-XL; BCL-XL/S; bcl2-L-1; BCL2L; BCL2L1; BCLX; Blc2l; PPP1R52

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UniProt ID: (Human) Q07817, (Mouse) Q64373, (Rat) P53563

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Entrez Gene ID: (Human) 598, (Mouse) 12048, (Rat) 24888

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功能

protein binding protein kinase binding identical protein binding protein homodimerization activity protein heterodimerization activity BH3 domain binding cysteine-type endopeptidase inhibitor activity involved in apoptotic process BH domain binding MDM2/MDM4 family protein binding

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