Invitrogen

C19orf10 Polyclonal Antibody, PeproTech®

Name: C19orf10 Polyclonal Antibody, PeproTech\u00AE
Brand: Invitrogen
SKU: 500-P259-1MG

查看其他10个C19orf10抗体

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

说明书

实验方案

问题与答案

技术支持

抗体检测数据 (3)

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C19orf10 Antibody (500-P259-1MG) in WB

Western Blot: To detect Murine SF-20 by Western Blot analysis C19orf10 Polyclonal Antibody (Product # 500-P259-1MG) can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine SF-20 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

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Immunogen Image C19orf10 Polyclonal Antibody, PeproTech®

产品信息

500-P259-1MG

应用

建议稀释比

已发表文章

免疫印迹 (WB)

0.1-0.2 µg/mL

酶联免疫吸附实验 (ELISA)

0.5-2.0 µg/mL

产品规格

种属反应

Mouse

宿主/亚型

Rabbit

分类

Polyclonal

类型

Antibody

抗原

E.coli-derived Recombinant Murine SF-20

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偶联物

Unconjugated Unconjugated Unconjugated

形式

Lyophilized

浓度

0.1-1.0 mg/mL

纯化类型

Antigen affinity chromatography

保存液

PBS

内含物

no preservative

保存条件

-20°C

运输条件

Ambient

AB_2930117

产品详细信息

AA Sequence of recombinant protein: MVSEPTTVPF DVRPGGVVHS FSQDVGPGNK FTCTFTYASQ GGTNEQWQMS LGTSEDSQHF TCTIWRPQGK SYLYFTQFKA ELRGAEIEYA MAYSKAAFER ESDVPLKSEE FEVTKTAVSH RPGAFKAELS KLVIVAKAAR SEL.

Preparation: Produced from sera of rabbits immunized with highly pure Recombinant Murine SF-20. Anti-Murine SF-20-specific antibody was purified by affinity chromatography employing an immobilized Murine SF-20 matrix.

Sandwich ELISA: To detect Murine SF-20 by sandwich ELISA (using 100 µL/well antibody solution) a concentration of 0.5-2.0 µg/mL of this antibody is required. This antigen affinity purified antibody, in conjunction with PeproTech Biotinylated Anti-Murine SF-20 (500-P259Bt) as a detection antibody, allows the detection of at least 0.2-0.4 ng/well of Recombinant Murine SF-20.

Western Blot: To detect Murine SF-20 by Western Blot analysis this antibody can be used at a concentration of 0.1-0.2 µg/mL. Used in conjunction with compatible secondary reagents the detection limit for Recombinant Murine SF-20 is 1.5-3.0 ng/lane, under either reducing or non-reducing conditions.

靶标信息

The interleukins are a broad family of well characterized cytokines, primarily of hematopoietic cell origin. Cytokines are small, soluble proteins with pleiotropic effects on a variety of cell types. Cytokines have a regulatory function over the immune system and mediate aspects of inflammatory response. IL-25 (also called SF20/IL-25) is a secreted bone marrow stroma-derived growth factor cytokine that is derived from Th2 T-cells. IL-25 belongs to the IL-17 cytokine family and is capable of amplifying allergic type inflammatory responses by its actions on other cell types. IL-25 binds to mouse thymic shared antigen-1 and supports lymphoid cell proliferation. Infusion of mice with IL-25 induces IL-4, IL-5, and IL-13 gene expression. IL-25 induced mice also develop epithelial cell hyperplasia, increased mucus secretion, and airway hyperreactivity, suggesting that IL-25 may be an important mediator of allergic disease via production of IL-4, IL-5, IL-13 and eotaxin.

仅用于科研。不用于诊断过程。未经明确授权不得转售。