Invitrogen

CAS Monoclonal Antibody (P729)

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CAS Antibody in Immunocytochemistry (ICC/IF) — Immunocytochemistry-Immunofluorescence analysis of CAS was performed in HeLa cells fixed in 4% paraformaldehyde at RT for 15 min. Green: CAS Monoclonal Antibody (P729) (Product # MA5-18303) diluted at 1:500. Red: phalloidin, a cytoskeleton marker. Scale bar = 10 µm.

CAS Antibody in Western Blot (WB) — CAS Monoclonal Antibody (P729) detects CSE1L protein by western blot analysis. A. 30 µg Neuro2A whole cell lysate/extract. B. 30 µg GL261 whole cell lysate/extract. C. 30 µg C8D30 whole cell lysate/extract. D. 30 µg NIH-3T3 whole cell lysate/extrac. E. 30 µg BCL-1 whole cell lysate/extrac. F. 30 µg Raw 264.7 whole cell lysate/extract. G. 30 µg C2Cl2 whole cell lysate/extract.7.5 % SDS-PAGE. CAS Monoclonal Antibody (P729) (Product # MA5-18303) dilution: 1:1,000.

产品信息

MA5-18303

应用	建议稀释比	已发表文章
免疫印迹 (WB)	1:500-1:3,000	-
免疫细胞化学 (ICC/IF)	1:100-1:1,000	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Mouse / IgG2a
分类	Monoclonal
类型	Antibody
克隆号	P729
抗原	Recombinant fragment corresponding to a region within amino acids 222 and 469 of CSE1L 查看免疫原
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Protein G
保存液	PBS
内含物	no preservative
保存条件	Store at 4°C short term. For long term storage, store at -20°C, avoiding freeze/thaw cycles.
RRID	AB_2539677

产品详细信息

Recommended positive controls: 293T, A431, HeLa, HepG2, Neuro2A, GL261, C8D30, NIH-3T3, BCL-1, Raw264.7, C2C12, PC-12, Rat2.

Predicted reactivity: Mouse (99%), Rat (100%), Zebrafish (95%), Xenopus laevis (97%), Pig (100%), Rhesus Monkey (100%), Bovine (100%).

Store product as a concentrated solution. Centrifuge briefly prior to opening the vial.

靶标信息

Proteins that carry a nuclear localization signal (NLS) are transported into the nucleus by the importin-alpha/beta heterodimer. Importin-alpha binds the NLS, while importin-beta mediates translocation through the nuclear pore complex. After translocation, RanGTP binds importin-beta and displaces importin-alpha. Importin-alpha must then be returned to the cytoplasm, leaving the NLS protein behind. The protein encoded by this gene binds strongly to NLS-free importin-alpha, and this binding is released in the cytoplasm by the combined action of RANBP1 and RANGAP1. In addition, the encoded protein may play a role both in apoptosis and in cell proliferation.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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引用该产品

生物信息学

蛋白别名: Cellular apoptosis susceptibility protein; Chromosome segregation 1-like protein; CSE1 chromosome segregation 1 like; CSE1 chromosome segregation 1-like; Exp2; Exportin-2; Importin-alpha re-exporter; MGC117283; MGC130036; MGC130037

基因别名: 2610100P18Rik; AA407533; Capts; CAS; CSE1; CSE1L; XPO2

UniProt ID: (Human) P55060, (Mouse) Q9ERK4

Entrez Gene ID: (Human) 1434, (Mouse) 110750, (Rat) 362273

分子生物学功能: transporter