Invitrogen

CASK Polyclonal Antibody

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CASK Antibody in Immunocytochemistry (ICC/IF) — Immunofluorescent analysis of CASK was performed using 70% confluent log phase U-87 MG cells. The cells were fixed with 4% paraformaldehyde for 10 minutes, permeabilized with 0.1% Triton™ X-100 for 10 minutes, and blocked with 1% BSA for 1 hour at room temperature. The cells were labeled with CASK Rabbit Polyclonal Antibody (Product # PA1-544) at 2 µg/mL in 0.1% BSA and incubated for 3 hours at room temperature and then labeled with Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, Alexa Fluor® 488 conjugate (Product # A27034) a dilution of 1:2000 for 45 minutes at room temperature (Panel a: green). Nuclei (Panel b: blue) were stained with SlowFade® Gold Antifade Mountant with DAPI (Product # S36938). F-actin (Panel c: red) was stained with Alexa Fluor® 555 Rhodamine Phalloidin (Product # R415, 1:300). Panel d represents the merged image showing cytoplasmic localization. Panel e shows the no primary antibody control. The images were captured at 60X magnification.

CASK Antibody in Western Blot (WB) — Western blot analysis was performed on tissue extracts (30 µg lysate) of Mouse Brain (Lane 1) and Rat Brain (Lane 2). The blot was probed with Anti-CASK Rabbit Polyclonal Antibody (Product # PA1-544, 0.5-2 µg/mL) and detected by chemiluminescence using Goat anti-Rabbit IgG (H+L) Superclonal™ Secondary Antibody, HRP conjugate (Product # A27036, 0.4 µg/mL, 1:2500 dilution). A 105 kDa band corresponding to CASK was observed across the tissues tested. Known quantity of protein samples were electrophoresed using Novex® NuPAGE® 10 % Bis-Tris gel (Product # NP0302BOX), XCell SureLock™ Electrophoresis System (Product # EI0002) and Novex® Sharp Pre-Stained Protein Standard (Product # LC5800). Resolved proteins were then transferred onto a nitrocellulose membrane with PierceTM Power Blotter System (Product # 22834). The membrane was probed with the relevant primary and secondary Antibody using iBind™ Flex Western Starter Kit (Product # SLF2000S). Chemiluminescent detection was performed using Pierce™ ECL Western Blotting Substrate (Product # 32106).

产品信息

PA1-544

应用	建议稀释比	已发表文章
免疫印迹 (WB)	0.5-2 µg/mL	-
免疫组化 (IHC)	Assay-dependent	-
免疫细胞化学 (ICC/IF)	2 µg/mL	-

产品规格
种属反应	Human, Mouse, Rat
宿主/亚型	Rabbit / IgG
分类	Polyclonal
类型	Antibody
抗原	Synthetic Peptide: C(578) E R D S P S T S R Q S P A N(592)
偶联物	Unconjugated
形式	Liquid
浓度	1 mg/mL
纯化类型	Antigen affinity chromatography
保存液	PBS with 1mg/mL BSA
内含物	0.05% sodium azide
保存条件	-20° C, Avoid Freeze/Thaw Cycles
RRID	AB_2243867

产品详细信息

PA1-544 detects calmodulin sensitive kinase (CASK) from mouse and rat tissues and cells.

PA1-544 has been successfully used in Western blot and immunohistochemistry procedures. By Western blot, this antibody detects a 112 kDa protein representing CASK from AtT20 cell lysate. Immunohistochemical staining of CASK in mouse brain with PA1-544 results in staining of the choroid plexus.

PA1-544 immunizing peptide corresponds to amino acid residues 578-592 from human CASK. This sequence is completely conserved between rat, mouse, and human CASK.

PA1-544 can be used with blocking peptide PEP-110.

靶标信息

This gene encodes a calcium/calmodulin-dependent serine protein kinase. The encoded protein is a MAGUK (membrane-associated guanylate kinase) protein family member. These proteins are scaffold proteins and the encoded protein is located at synapses in the brain. Mutations in this gene are associated with FG syndrome 4, mental retardation and microcephaly with pontine and cerebellar hypoplasia, and a form of X-linked mental retardation. Multiple transcript variants encoding different isoforms have been found for this gene.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

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生物信息学

蛋白别名: calcium/calmodulin-dependent serin protein kinase; Calcium/calmodulin-dependent serine protein kinase; calcium/calmodulin-dependent serine protein kinase (MAGUK family); calcium/calmodulin-dependent serine protein kinase membrane-associated guanylate kinase; hCASK; MGC7449; OTTHUMP00000023157; OTTHUMP00000023161; OTTHUMP00000023162; Peripheral plasma membrane protein CASK; Protein lin-2 homolog; RP23-278L4.1; trinucleotide repeat containing 8

基因别名: CAGH39; CAMGUK; CASK; CMG; DXPri1; DXRib1; FGS4; LIN-2; LIN2; MICPCH; mLin-2; MRXSNA; Pals3; TNRC8

UniProt ID: (Human) O14936, (Rat) Q62915, (Mouse) O70589

Entrez Gene ID: (Human) 8573, (Rat) 29647, (Mouse) 12361

分子生物学功能: non-receptor serine/threonine protein kinase scaffold/adaptor protein