Invitrogen

CCDC53 Polyclonal Antibody

Name: CCDC53 Polyclonal Antibody
Brand: Invitrogen
SKU: PA5-100429
Availability: InStock

查看其他3个CCDC53抗体

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

说明书

Certificates

实验方案

问题与答案

技术支持

Additional Information:

抗体检测数据 (3)

CCDC53 Antibody in Immunocytochemistry (ICC/IF)

图: 1 / 3

CCDC53 Antibody (PA5-100429) in ICC/IF

Immunofluorescent analysis of CCDC53 in HeLa cells. Samples were fixed with paraformaldehyde, permeabilized with 0.1% Triton X-100, blocked with 10% serum (45 min at 25°C), incubated with mouse anti-beta tubulin and CCDC53 polyclonal antibody (Product # PA5-100429) using a dilution of 1:200 (1 hr, 37°C), and followed by goat anti-rabbit IgG Alexa Fluor 594 (red) and goat anti-mouse IgG Alexa Fluor 488 (green).

Please note: We are reviewing Western blot images included in the antibody testing data in our catalog, including those provided by third parties. Unless expressly labeled or annotated as “raw-unedited”, Western blot images included in the antibody testing data in our catalog may have been edited, optimized or otherwise adjusted for presentation.

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Immunogen image

Immunogen Image CCDC53 Polyclonal Antibody

产品信息

PA5-100429

应用

建议稀释比

已发表文章

免疫印迹 (WB)

1:500-1:2,000

免疫细胞化学 (ICC/IF)

1:100-1:500

产品规格

种属反应

Human, Mouse, Rat

宿主/亚型

Rabbit / IgG

分类

Polyclonal

类型

Antibody

抗原

A synthesized peptide derived from human WASHC3(Accession Q9Y3C0), corresponding to amino acid residues V143-S193.

3D表位/免疫原

偶联物

Unconjugated Unconjugated Unconjugated

形式

Liquid

浓度

1 mg/mL

100 µg

纯化类型

Affinity chromatography

保存液

PBS, pH 7.4, with 50% glycerol

内含物

0.02% sodium azide

保存条件

-20°C

运输条件

Ambient (domestic); Wet ice (international)

AB_2849940

产品详细信息

Antibody detects endogenous levels of total CCDC53.

靶标信息

Acts as a component of the WASH core complex that functions as a nucleation-promoting factor (NPF) at the surface of endosomes, where it recruits and activates the Arp2/3 complex to induce actin polymerization, playing a key role in the fission of tubules that serve as transport intermediates during endosome sorting. [UniProt]

仅用于科研。不用于诊断过程。未经明确授权不得转售。