Invitrogen

CCL2 (MCP-1) Monoclonal Antibody (2H5), Functional Grade, eBioscience™

CCL2 (MCP-1) Antibody in Immunocytochemistry (ICC/IF)

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CCL2 (MCP-1) Antibody (16-7096-81) in ICC/IF

Journal of extracellular vesicles 2021 - 8 FIGURE CRT mediates efferocytosis of MSC-derived apoVs to modulate T2D liver macrophages in vivo. (a) Representative immunofluorescent (IF) staining images of F4/80 (green) and CD11b (green) in the liver, counterstained by Hoechst (blue). DIO, mice with diet-induced obesity; si-NC-apoV, DIO mice treated by apoVs derived from MSCs transfected by siRNA-negative control; si- CRT -apoV, DIO mice treated by ap... View More

CCL2 (MCP-1) Antibody in Immunohistochemistry (IHC)

CCL2 (MCP-1) Antibody in Flow Cytometry (Flow)

CCL2 (MCP-1) Antibody in Neutralization (Neu)

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产品信息

16-7096-81

应用

建议稀释比

已发表文章

免疫组化 (IHC)

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免疫细胞化学 (ICC/IF)

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流式细胞分析 (Flow)

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酶联免疫吸附实验 (ELISA)

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中和实验 (Neu)

Assay-Dependent

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功能检测 (Functional)

Assay-Dependent

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产品规格

种属反应

Human, Mouse, Rat

已发表种属

Human, Mouse

宿主/亚型

Armenian hamster / IgG

分类

Monoclonal

类型

Antibody

克隆号

2H5

偶联物

Functional Grade Functional Grade Functional Grade

形式

Liquid

浓度

1 mg/mL

纯化类型

Affinity chromatography

保存液

PBS, pH 7.2

内含物

no preservative

保存条件

4° C

运输条件

Ambient (domestic); Wet ice (international)

RRID

AB_469220

产品详细信息

Description: The 2H5 monoclonal antibody reacts with mouse, rat, and human monocyte chemoattractant protein-1 (MCP-1), also known as CCL2 and MCAF.

Applications Reported: The 2H5 antibody has been reported for use in ELISA, intracellular staining for flow cytometric analysis, and cytokine neutralization. .

Applications Tested: The Functional Grade purified 2H5 antibody has been tested by ELISA for verification of reactivity and by LAL assay for verification of low endotoxin. The 2H5 antibody has been published for use in vitro and in vivo for neutralization of MCP-1 activity.

Mouse MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of mouse MCP-1 in combination with the affinity purified 4E2/MCP (14-7091) antibody for capture and recombinant mouse MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

Human MCP-1 ELISA: The biotinylated 2H5 antibody has been tested as the detection antibody in a sandwich ELISA for analysis of human MCP-1 in combination with the affinity purified 5D3-F7 (14-7099) antibody for capture and recombinant human MCP-1 as the standard. A suitable range of concentrations of this antibody for ELISA detection is 0.5-2.0 µg/mL. A standard curve consisting of doubling dilutions of the recombinant standard over the range of 2000 pg/mL - 15 pg/mL should be included in each ELISA plate.

Storage and handling: Use in a sterile environment.

Filtration: 0.2 µm post-manufacturing filtered.

Purity: Greater than 90%, as determined by SDS-PAGE.

Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.

Aggregation: Less than 10%, as determined by HPLC.

靶标信息

This gene is one of several cytokine genes clustered on the q-arm of chromosome 17. Cytokines are a family of secreted proteins involved in immunoregulatory and inflammatory processes. The protein encoded by this gene is structurally related to the CXC subfamily of cytokines. Members of this subfamily are characterized by two cysteines separated by a single amino acid. This cytokine displays chemotactic activity for monocytes and basophils but not for neutrophils or eosinophils. It has been implicated in the pathogenesis of diseases characterized by monocytic infiltrates, like psoriasis, rheumatoid arthritis and atherosclerosis. It binds to chemokine receptors CCR2 and CCR4.

仅用于科研。不用于诊断过程。未经明确授权不得转售。

Product References

Mural cell-derived chemokines provide a protective niche to safeguard vascular macrophages and limit chronic inflammation.

Immunity

Pekayvaz K,Gold C,Hoseinpour P,Engel A,Martinez-Navarro A,Eivers L,Coletti R,Joppich M,Dionísio F,Kaiser R,Tomas L,Janjic A,Knott M,Mehari F,Polewka V,Kirschner M,Boda A,Nicolai L,Schulz H,Titova A,Kilani B,Lorenz M,Fingerle-Rowson G,Bucala R,Enard W,Zimmer R,Weber C,Libby P,Schulz C,Massberg S,Stark K

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 16-7096-81) in Neutralization

Tue Oct 10 00:00:00 EDT 2023

Mas receptor activation attenuates allergic airway inflammation via inhibiting JNK/CCL2-induced macrophage recruitment.

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Hong L,Wang Q,Chen M,Shi J,Guo Y,Liu S,Pan R,Yuan X,Jiang S

16-7096-81 was used in Neutralization to suggest that Mas receptor activation significantly attenuated CCL2 dependent macrophage recruitments in acute allergic airway inflammation through JNK pathways, which indicated that Mas receptor, CCL2 and phospho-JNK could be potential targets against allergic airway inflammation.

Sat May 01 00:00:00 EDT 2021

Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes.

Journal of extracellular vesicles

Zheng C,Sui B,Zhang X,Hu J,Chen J,Liu J,Wu D,Ye Q,Xiang L,Qiu X,Liu S,Deng Z,Zhou J,Liu S,Shi S,Jin Y

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 16-7096-81) in Immunohistochemistry

Sat May 01 00:00:00 EDT 2021

Apoptotic vesicles restore liver macrophage homeostasis to counteract type 2 diabetes.

Journal of extracellular vesicles

Zheng C,Sui B,Zhang X,Hu J,Chen J,Liu J,Wu D,Ye Q,Xiang L,Qiu X,Liu S,Deng Z,Zhou J,Liu S,Shi S,Jin Y

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 16-7096-81) in Immunohistochemistry

Sat May 01 00:00:00 EDT 2021

Renal Sodium Gradient Orchestrates a Dynamic Antibacterial Defense Zone.

Cell

Berry MR,Mathews RJ,Ferdinand JR,Jing C,Loudon KW,Wlodek E,Dennison TW,Kuper C,Neuhofer W,Clatworthy MR

16-7096-81 was used in Functional assay to reveal a critical accessory role for the homeostatic function of a vital organ in optimizing tissue defense.

Thu Aug 24 00:00:00 EDT 2017

Brain Invasion by Mouse Hepatitis Virus Depends on Impairment of Tight Junctions and Beta Interferon Production in Brain Microvascular Endothelial Cells.

Journal of virology

Bleau C,Filliol A,Samson M,Lamontagne L

16-7096 was used in an ELISA assay to investigate how Coronaviruses exhibit the ability to cross the blood-brain barrier according to strain virulence.

Thu Oct 01 00:00:00 EDT 2015

Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis.

Molecular medicine reports

Shen C,Lie P,Miao T,Yu M,Lu Q,Feng T,Li J,Zu T,Liu X,Li H

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 16-7096-81) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

Conditioned medium from umbilical cord mesenchymal stem cells induces migration and angiogenesis.

Molecular medicine reports

Shen C,Lie P,Miao T,Yu M,Lu Q,Feng T,Li J,Zu T,Liu X,Li H

Published figure using CCL2 (MCP-1) monoclonal antibody (Product # 16-7096-81) in Flow Cytometry

Wed Jul 01 00:00:00 EDT 2015

The role of the Src family kinase Lyn in the immunomodulatory activities of cathelicidin peptide LL-37 on monocytic cells.

Journal of leukocyte biology

Nijnik A,Pistolic J,Cho P,Filewod NC,Falsafi R,Ramin A,Harder KW,Hancock RE

16-7096 was used in an ELISA assay to elucidate the common molecular mechanisms mediating the chemokine induction activity of natural and synthetic cationic peptides in monocytic cells.

Sun Apr 01 00:00:00 EDT 2012

Synthetic cationic peptide IDR-1002 provides protection against bacterial infections through chemokine induction and enhanced leukocyte recruitment.

Journal of immunology (Baltimore, Md. : 1950)

Nijnik A,Madera L,Ma S,Waldbrook M,Elliott MR,Easton DM,Mayer ML,Mullaly SC,Kindrachuk J,Jenssen H,Hancock RE

16-7096 was used in an ELISA assay to study the importance of the chemokine induction activity of host defence peptides in protection against bacterial infections.

Mon Mar 01 00:00:00 EST 2010

生物信息学

蛋白别名: C-C motif chemokine 2; chemokine (C-C motif) ligand 2; H-MCP-1; HC11; immediate-early serum-responsive JE protein; Immediate-early serum-responsive protein JE; MCAF; MCP-1; MGC9434; Monocyte chemoattractant protein 1; monocyte chemoattractant protein-1; Monocyte chemotactic and activating factor; Monocyte chemotactic protein 1; Monocyte secretory protein JE; Platelet-derived growth factor-inducible protein JE; RP23-350G1.3; small inducible cytokine A2; small inducible cytokine A2 (monocyte chemotactic protein 1, homologous to mouse Sig-je); small inducible cytokine subfamily A (Cys-Cys), member 2; small inducible gene JE; Small-inducible cytokine A2

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基因别名: AI323594; CCL2; GDCF-2; HC11; HSMCR30; JE; MCAF; MCP-1; MCP1; SCYA2; Sigje; SMC-CF

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UniProt ID: (Human) P13500, (Rat) P14844, (Mouse) P10148

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Entrez Gene ID: (Human) 6347, (Rat) 24770, (Mouse) 20296

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功能

protein kinase activity receptor binding chemokine activity heparin binding CCR2 chemokine receptor binding G-protein coupled receptor binding cytokine activity protein binding cytokine intercellular signal molecule

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